Biais, Benot Bénard, Camille Beauvoit, Bertrand Colombié, Sophie Prodhomme, Duyên Ménard, Guillaume Bernillon, Stéphane Gehl, Bernadette Gautier, Hélène Ballias, Patricia
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Published in
PLANT PHYSIOLOGY
To assess the influence of the environment on fruit metabolism, tomato (Solanum lycopersicum 'Moneymaker') plants were grown under contrasting conditions (optimal for commercial, water limited, or shaded production) and locations. Samples were harvested at nine stages of development, and 36 enzyme activities of central metabolism were measured as w...
Besseau, Sébastien Kellner, Franziska Lanoue, Arnaud Thamm, Antje M K Salim, Vonny Schneider, Bernd Geu-Flores, Fernando Höfer, René Guirimand, Grégory Anthony Guihur
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Published in
PLANT PHYSIOLOGY
Hydroxylation of tabersonine at the C-16 position, catalyzed by tabersonine 16-hydroxylase (T16H), initiates the synthesis of vindoline that constitutes the main alkaloid accumulated in leaves of Catharanthus roseus. Over the last decade, this reaction has been associated with CYP71D12 cloned from undifferentiated C. roseus cells. In this study, we...
Wan, Yinglang Jasik, Jan Wang, Li Hao, Huaiqing Volkmann, Dieter Menzel, Diedrik Mancuso, Stefano Baluška, František Lin, Jinxing
Published in
The Plant Cell
Under blue light (BL) illumination, Arabidopsis thaliana roots grow away from the light source, showing a negative phototropic response. However, the mechanism of root phototropism is still unclear. Using a noninvasive microelectrode system, we showed that the BL sensor phototropin1 (phot1), the signal transducer NONPHOTOTROPIC HYPOCOTYL3 (NPH3), a...
Saidi, Younousse Finka, Andrija Muriset, Maude Bromberg, Zohar Weiss, Yoram G Maathuis, Frans J M Goloubinoff, Pierre
Published in
The Plant cell
Land plants are prone to strong thermal variations and must therefore sense early moderate temperature increments to induce appropriate cellular defenses, such as molecular chaperones, in anticipation of upcoming noxious temperatures. To investigate how plants perceive mild changes in ambient temperature, we monitored in recombinant lines of the mo...
Huang, Shaobai Taylor, Nicolas L Whelan, James Millar, A Harvey
Published in
Plant physiology
Mitochondrial protein import is a complex multistep process from synthesis of proteins in the cytosol, recognition by receptors on the organelle surface, to translocation across one or both mitochondrial membranes and assembly after removal of the targeting signal, referred to as a presequence. In plants, import has to further discriminate between ...
Cai, Wenhe Ji, Daili Peng, Lianwei Guo, Jinkui Ma, Jinfang Zou, Meijuan Lu, Congming Zhang, Lixin
Published in
Plant physiology
To gain insight into the molecular mechanism of RNA editing, we have characterized the low psii accumulation66 (lpa66) Arabidopsis (Arabidopsis thaliana) mutant, which displays a high chlorophyll fluorescence phenotype. Its perturbed chlorophyll fluorescence is reflected in reduced levels of photosystem II (PSII) proteins. In vivo protein labeling ...
Canino, Giusy Bocian, Edyta Barbezier, Nicolas Echeverría, Manuel Forner, Joachim Binder, Stefan Marchfelder, Anita
Published in
Plant physiology
Functional transfer RNA (tRNA) molecules are a prerequisite for protein biosynthesis. Several processing steps are required to generate the mature functional tRNA from precursor molecules. Two of the early processing steps involve cleavage at the tRNA 5' end and the tRNA 3' end. While processing at the tRNA 5' end is performed by RNase P, cleavage ...
Rivero, Rosa M Shulaev, Vladimir Blumwald, Eduardo
Published in
Plant physiology
We investigated the effects of P(SARK)IPT (for Senescence-Associated Receptor KinaseIsopentenyltransferase) expression and cytokinin production on several aspects of photosynthesis in transgenic tobacco (Nicotiana tabacum cv SR1) plants grown under optimal or restricted (30% of optimal) watering regimes. There were no significant differences in sto...
Waters, Mark T Wang, Peng Korkaric, Muris Capper, Richard G Saunders, Nigel J Langdale, Jane A
Published in
The Plant cell
Chloroplasts of photosynthetic organisms harness light energy and convert it into chemical energy. In several land plants, GOLDEN2-LIKE (GLK) transcription factors are required for chloroplast development, as glk1 glk2 double mutants are pale green and deficient in the formation of the photosynthetic apparatus. We show here that glk1 glk2 double mu...
Richard L. Sabina Anna-Lisa Paul Robert J. Ferl Bernd Laber Stephen D. Lindell
AMP deaminase (AMPD) is essential for plant life, but the underlying mechanisms responsible for lethality caused by genetic and herbicide-based limitations in catalytic activity are unknown. Deaminoformycin (DF) is a synthetic modified nucleoside that is taken up by plant cells and 5′-phosphorylated into a potent transition state-type inhibitor of ...