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Biology is increasingly data driven by virtue of the development of high-throughput technologies such as DNA and RNA sequencing Computational biology and bioinformatics are scientific disciplines that cross-over between the disciplines of biology informatics and statistics which is clearly reflected in this thesis Bioinformaticians often contribute crucial insights and novelty to scientific research because they are central to data analysis and contribute concrete algorithms and software solutions In addition bioinformaticians have an important role to play when it comes to organising data and software and making it accessible to others In this thesis in addition to contributing to biological questions I discuss issues around accessing and sharing data with the challenges of handling large data input output IO bottlenecks and effective use of multi-core computations By creating software solutions together with molecular biologists I contributed and published insights in biological processes in nematodes and plants I published software solutions that made it easier for others to analyse data which impacts the wider research community I created solutions that made it easier for others to publish software solutions by themselves The introduction of computing and the internet makes it possible to share ideas and computational methods I am convinced it is a good idea to publish software solutions as free and open source software FOSS in the public domain so that we can continue to build on the work of others Chapter 2 presents a computational method for identifying gene families in a sequenced genome that may be involved in pathogenicity i e those genes that code for proteins that interact with molecules of an infected host Such nematode proteins are known to contain highly variable DNA sections that code for the biochemical properties of an interaction site By applying phylogenetic analysis through maximum likelihood PAML and comparison of homologues sequences in other organisms with comparable and different life styles we discovered 77 unique candidate sequence families in the plant pathogen Meloidogyne incognita that deserve further investigation in the laboratory Chapter 3 presents GenEST a computational method for predicting which fragments captured by the cDNA-AFLP high-throughput technology matched known expressed sequence tags ESTs The cDNA-AFLP biochemical process was calculated in silico and fragments matching the fragment lengths as given by cDNA-AFLP were matched Through this technique novel effectors from the nematode Globodera rostochiensis putatively involved in pathogenicity were identified and partly confirmed in the laboratory Chapter 4 presents GenFrag a computational method that expands on GenEST for predicting which fragments captured by cDNA-AFLP matched fragments of a fully sequenced genome with its known spliced gene variants Through this in silico technique genes were identified in the plant Arabidopsis thaliana putatively involved in maternal genomic imprinting and partly confirmed in the laboratory Chapter 5 presents multiple QTL mapping MQM a high-throughput computational method for predicting what sections of a genome correlate with for example gene expression The study of finding such eQTL is challenging not least because many of them are potentially false positives The MQM parallelized algorithm is embedded in the R qtl software package which makes it widely available to researchers The impact thereof means that it is widely cited by studies on model organisms such as mouse rat the nematode Caenorhabditis elegans and the plant A thaliana Chapter 6 presents a theoretical framework in the form of a review for identifying plant-resistance genes R-genes that combines the lessons learnt in the previous chapters Plants lack an adaptive immune system and therefore next to having physical defences use R-genes to code for proteins that recognise molecules and proteins from invading pathogens with an example on A thaliana These R-genes can be viewed as the counterparts of effectors identified in Chapter 3 and Chapter 4 By introducing the concept of a prior the chapter discusses eQTL or broader xQTL techniques as presented in the Chapter 5 to narrow down on gene candidates involved in plant defence Chapter 7 and Chapter 8 present FOSS bioinformatics tools and modules that make use the Ruby programming language BioRuby Chapter 7 has components for sequence analysis pathway analysis protein modelling and phylogenetic analysis it supports widely used data formats and provides access to databases external programs and public web services All Ruby software created in the context of this thesis was contributed initially to the main BioRuby project e g the PAML parser of Chapter 2 and later as individual Biogems Chapter 8 e g the bio-blastxmlparser bio-alignment bigbio and bio-rdf biogems for Chapter 2 and three Genfrag related biogems for Chapter 4 Over 16 modules were contributed by the author as Ruby FOSS projects and are listed on the http biogems info website Because of the open nature of the BioRuby project both BioRuby and BioGem software modules are increasingly used and cited in biomedical research not only in genomics but also in phylogenetics and prediction of protein structural complexes and data integration Chapter 9 presents sambamba a software tool for scaling up next generation sequencing NGS alignment processing through the use of multiple cores on a computer Sambamba is a replacement for samtools a commonly used software tool for working with aligned output from sequencers Sambamba makes use of multi-core processing and is written in the D programming language Not only does sambamba outperform samtools but it already comes with an improved deduplication routine and other facilities such as easy filtering of data The Sambamba software is now used in the large sequencing centres around the world Chapter 10 Big Data but are we ready gives a response to a publication on using cloud computing for large data processing The chapter discusses computational bottlenecks and proves prescient because the number of citations of this paper increases every year Chapter 11 Towards effective software solutions for big biology discusses the need for a change of strategy with regard to bioinformatics software development in the biomedical sciences to realise big biology software projects This includes improved scientific career tracks for bioinformaticians and dedicated funding for big data software development Chapter 12 discusses the computational methods and software solutions presented in this thesis painting a picture of further challenges in bioinformatics computational solutions for the elucidation of biological processes The chapter starts with a discussion on the merits and shortcomings of each individual software solution presented in this thesis followed by a perspective on next generation sequencing data integration and future research in software solutions

Ophaalcriteria op high-techbedrijf onder de loep

Wachtruimte effectief benutten

High-throughput open source computational methods for genetics and genomics

Publication search with verwerkingscapaciteit as keyword

Ophaalcriteria op high-techbedrijf onder de loep

Wachtruimte effectief benutten

High-throughput open source computational methods for genetics and genomics

Publication search
with verwerkingscapaciteit as keyword