Published in The Annals of Thoracic Surgery
Background. The heparin protocols used during cardiopulmonary bypass (CPB) in children undergoing surgical repair for congenital heart disease are extrapolated from adult data. Studies are needed that assess the optimal heparin dosing in these children, whose heparin clearance is increased compared with that in adults. Methods. We assessed the effe...
Published in Journal of Thrombosis and Thrombolysis
Thrombin contributes to the pathogenesis of acute myocardial infarction and reocclusion after thrombolysis. Thrombolytic therapy is known to induce a paradoxic increase in thrombin generation. Specific thrombin inhibition enhances thrombolytic therapy in experimental models. The aim of this study was to determine thrombin generation in patients wit...
Published in Cardiovascular Toxicology
The laboratory assessment of platelet biology and coagulation has traditionally focused on abnormalities, congenital or acquired, that provide insights for understanding hemostatic defects, unexplained bleeding, and pharmacologic titration of compounds designed to attenuate thrombotic potential. Because atherosclerotic vascular disease is associate...
Published in Biochemistry (Moscow)
The process of tissue factor initiated blood coagulation is discussed. Reactions of the blood coagulation cascade are propagated by complex enzymes containing a vitamin K-dependent serine protease and an accessory cofactor protein that are assembled on a membrane surface in a calcium-dependent manner.These complexes are 105 109-fold more efficient ...
Diamond-like carbon (DLC) and silicon carbide (SiC) coat- ings are attractive because of low friction coefficient, high hardness, chemical inertness and smooth finish, which they provide to biomedical devices. Silicon wafers (Si and silicone rubber (Si) plates were coated using plasma- enhanced chemical vapour deposition (PE-CVD) tech- niques. This...
Published in Cell Biochemistry and Biophysics
This article reviews work performed at the Medical College of Virginia of Virginia Commonwealth University during the development of a whole-blood assay of platelet function. The new assay is capable of assessing platelet function during clotting and thus allows measurement of the contribution of platelets to thrombin generation. Because platelets ...
Published in Thrombosis Journal
BackgroundIt is well described that diabetes mellitus is a hypercoagulable state. It is also known that patients with renal dysfunction have impaired platelet aggregation and function. It is not well described how renal dysfunction affects the hypercoagulability associated with diabetes. This post-hoc sub-group analysis compares platelet function, ...
Lupus anticoagulants (LAC) are associated with thromboembolic complications (TEC). LAC can be detected via their anticoagulant properties in coagulation tests and thrombin generation assays, via the peak height (PH) and lag time (LT). To assess the thrombotic risk in LAC-positive patients, we have presently expressed the LAC-activity quantitatively...
Published in American Journal of Obstetrics and Gynecology
ObjectiveNormal pregnancy results in a prothrombotic state. Studies that have investigated the capacity of pregnant women to generate thrombin are limited. Our aim was to evaluate thrombin generation longitudinally from the preconception period, through pregnancy, and after pregnancy. Study DesignWe evaluated young, healthy nulligravid women (n = 2...
Published in International journal of burns and trauma
Theses data show that thrombin generation is affected by hypothermia, but not 40% dilution. TEG is affected by 40% dilution with Hextend, but not by hypothermia. PT and aPTT are significantly affected by both hypothermia and dilution. Recombinant FVIIa caused a greater change in thrombin generation at 34°C as compared to 37°C, and a greater change ...
