Published in Surgery Today
The purpose of this study was to evaluate the effect of prostaglandin E1 (PGE1) on protecting against hepatic endothelial cell damage and increasing graft viability after cold preservation and reperfusion, using an isolated perfused rat liver (IPRL) model. The grafts were divided into three groups, according to the cold preservation time and PGE1 a...
Published in Journal of Hepato-Biliary-Pancreatic Surgery
The effects of intraportal administration of prostaglandin E1 (PGE1) on portal venous flow, hepatic arterial flow, peripheral tissue blood flow, and systemic arterial flow before and after 60 min total liver ischemia followed by 70% partial hepatectomy in rats were investigated. Total liver ischemia was induced by occluding the hepatoduodenal ligam...
Published in Surgery Today
(PGE1) and tyrosine kinase inhibitors on hepatic energy status and protein synthesis in ischemic livers were studied using 31P-magnetic resonance spectroscopy in a rat model. The continuous administration of PGE1 significantly increased the β-adenosine triphosphate/inorganic phosphate (β-ATP/Pi) ratio and hepatic protein synthesis rate (HPS) after ...
Published in Biochemical Pharmacology
Since cyclic 3',5'-adenosine monophosphate (cAMP) is involved in cell proliferation and as previous data showed that imidazo[1,2-α]pyrazine derivatives (PAB12, PAB30, PAB40, SCA40, SCA41, and SCA44) inhibited cAMP breakdown by a phosphodiesterase (PDE)-inhibitory effect, the aim of the present study was to investigate the effects of these derivativ...
Published in Biochemical Pharmacology
Effects of the transmethylation inhibitors 3-deazaadenosine (c 3Ado) and 3-deaza-(±)-aristeromycin (c 3Ari) on cell cycle and cyclic AMP (cAMP) concentrations in human promyelocytic leukemia cells (HL-60) were studied by flow cytometry and radioimmunoassay techniques. Previously described cell cycle accumulations, after incubation with drugs (25 μM...
Published in BBA - Biomembranes
A high-voltage discharge procedure has been developed for permeabilising the plasma membranes of both human and rat blood platelets. The cells can be resealed by incubation at 37°C, show 85% of rat platelets, and close to 100% human platelets, contained the encapsulated dye. The initial rates and % aggregation responses of both human and rat platel...
Published in Biochemical and Biophysical Research Communications
Neurotensin, bradykinin and somatostatin inhibited in a time- and concentration-dependent manner prostaglandin E1- or forskolin-stimulated cAMP production in neuroblastoma N1E115 cells. Cell treatment with 1 μg/ml pertussis toxin for 6 hours reversed the inhibition elicited by peptides after short incubation periods (- 3 min). Fluoroaluminate also ...
Eleven cystic fibrosis children (mean age, 9.6 years) were chosen at random to participate in a study to observe the effects of concurrently stimulating gastric/duodenal bicarbonate secretion and inhibiting gastric acid secretion, using a methylated prostaglandin E1 analogue in patients with pancreatic insufficiency and taking pancreatic enzymes. P...
Published in Biochemical and Biophysical Research Communications
Stimulation of NG115-401L neuronal cells with bradykinin produces a dose-dependent increase in inositol phosphate production which is not blocked, rather slightly increased, after treatment with pertussis toxin. Nevertheless, pertussis toxin stimulates ADP-ribosylation of a 41K membrane protein, and blocks opioid receptor-mediated inhibition of sti...
The portal vein system was clearly visualized in superior mesenteric arterial portography using prostaglandin E1. Angiographic examination was performed in 68 patients with various liver diseases during the 2 year period from 1980 to 1981. Twenty microgram of prostaglandin E1 was injected into the superior mesenteric artery 30 seconds before inject...
