Basford, R.E.
Published in
BBA - Biochimica et Biophysica Acta
Analysis of the lipid portion of coenzyme Q lipoprotein shows it to be 5–8% neutral lipid and 92–95% phospholipid. The neutral fraction contains coenzyme Q, cholesterol, probably glycerides(s) and at least three unknown components. The phospholipid fraction is comprised of cephalins and lecithins with a small amount of inositide. Both the cephalin ...
Gilbert, Lawrence I.
Published in
Advances in Insect Physiology
One of the fundamental questions concerning the role of lipids in the physiology of the insect concerns the quantity of lipid contained upon, and interior to, the rigid exoskeleton. Lipids are of vital importance to many insects as substrates for embryogenesis, metamorphosis and flight. Although several problems of the function and metabolism of li...
Hodges, T K Leonard, R T Bracker, C E Keenan, T W
Published in
Proceedings of the National Academy of Sciences of the United States of America
A membrane-bound adenosine triphosphatase (EC 3.6.1.3) that requires Mg(++) and that is stimulated by monovalent ions has been purified 7- to 8-fold from homogenates of oat (Avena sativa L. Cult. Goodfield) roots by discontinuous sucrose-gradient centrifugation. The enzyme was substrate specific; adenosine triphosphate was hydrolyzed 25 times more ...
Sparrow, J T Gotto, A M Jr Morrisett, J D
Published in
Proceedings of the National Academy of Sciences of the United States of America
Apolipoprotein-alanine is an apolipoprotein isolated from very-low-density lipoproteins of human plasma. This protein contains 79 amino acids and binds phosphatidyl choline. Four fragments of this molecule corresponding to sequence positions 41-79 (I), 48-79 (II), 55-79 (III), and 61-79 (IV) have been synthesized by standard solid-phase methods. Th...
Dix, J A Diamond, J M Kivelson, D
Published in
Proceedings of the National Academy of Sciences of the United States of America
The translational diffusion coefficient and the partition coefficient of a spin-labeled solute, di-t-butyl nitroxide, in an aqueous suspension of dipalmitoyl lecithin vesicles have been studied by electron spin resonance spectroscopy. When the lecithin is cooled through its phase transition temperature near 41 degrees C, some solute is "frozen out"...
Tsong, T Y Yang, C S
Published in
Proceedings of the National Academy of Sciences of the United States of America
Binding of benzphetamine to purified microsomal cytochrome P-450 from rat liver causes a shift in the heme spin state of the protein to favor the high-spin form. This shift is strongly temperature dependent. A rapid temperature jump of a cytochrome P-450/epsilon benzphetamine mixture, monitored by changes in the Soret absorptions of the heme, revea...
Yu, C.A. Gu, L-Q. Yu, L.
Published in
Biochemical and Biophysical Research Communications
Several azido-ubiquinones have been synthesized for the study of protein-ubiquinone interaction in succinate-cytochrome c reductase. In the absence of light, azido-ubiquinones are partially effective in restoring enzymatic activity to ubiquinone- and phospholipid-depleted reductase and the binding of azido-ubiquinones can be partially reversed by 5...
Shahrokh, Zahra Nichols, Alex V.
Published in
Biochemical and Biophysical Research Communications
Incubation of plasma (37°C, 6hr) in the presence of increasing amounts of phosphatidylcholine (PC) vesicles, above a threshold concentration, results in an increase in particle diameter of LDL relative to that from nonincubated plasma. With further PC addition, the major peak of LDL in the gradient gel electrophoretic pattern is transformed, first,...
Kiselev, G.V.
Published in
Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
A simple preparative method for Chromatographic isolation of pure fractions of di- and triphosphoinositides (1-phosphatidylinositol 4-phosphate and 1-phosphatidylinositol 4,5-bisphosphate) from ox brain is described. Polyphosphoinositide fractions have been obtained by ion-exchange chromatography of the lipid extract using gradient elution with 0-0...
Slotboom, A.J. Verheij, H.M. DeHaas, G.H.
Published in
New Comprehensive Biochemistry
Phospholipase A2 (PLA) catalyzes the reaction: diacyl phospholipid → monoacylphospholipid + fatty acid. The liberation of fatty acids is more easily quantitated; the most widely used method utilizes titration in a pH stat. Both purified lecithin and whole egg-yolk have been used, either with or without detergent. PLAs isolated from all sources are ...
