Celis, M E Taleisnik, S Walter, R
Published in
Proceedings of the National Academy of Sciences of the United States of America
Microsomal preparations from the stalk median eminence of female rats are shown to contain an enzymic activity that is responsible for the formation of MSH-release-inhibiting factor (MSH-R-IF). The amount of this activity remains constant throughout the estrous cycle. The corresponding mitochondrial preparations from the stalk median eminence conta...
Dousa, T Hechter, O Schwartz, I L Walter, R
Published in
Proceedings of the National Academy of Sciences of the United States of America
The investigation was undertaken to evaluate the direct stimulatory effects of neurohypophyseal hormones upon adenylate cyclase activity in a cell-free, particulate fraction derived from the kidney medulla of various mammalian species. The relative affinity of neurohypophyseal hormones for the receptor component of the adenylate cyclase system (as ...
Capra, J D Kehoe, J M Kotelchuck, D Walter, R Breslow, E
Published in
Proceedings of the National Academy of Sciences of the United States of America
The sequence of the first 50 amino-acid residues of bovine neurophysin-I was determined. A comparison of this sequence with that of the 97-residue bovine neurophysin-II and the 92-residue porcine neurophysin-I molecules reveals a high degree of homology among these proteins. It is suggested that the binding site of neurophysin proteins for neurohyp...
Glickson, J D Urry, D W Havran, R T Walter, R
Published in
Proceedings of the National Academy of Sciences of the United States of America
Proton nuclear magnetic resonances of two analogs of the mammalian antidiuretic hormone, lysine vasopressin, have been assigned. The analogs studied were deamino-lysine vasopressin and deamino-8-tosyllysine vasopressin, formally derived from lysine vasopressin by removal of the potential cationic sites. Most assignments made in deuterated dimethyls...
Vanderhaeghen, J J Lotstra, F De Mey, J Gilles, C
Published in
Proceedings of the National Academy of Sciences of the United States of America
The distribution of gastrin-cholecystokinin-like peptide(s) is reported in brain and hypophysis of the rat. The unlabeled peroxidase-antiperoxidase complex immunohistochemical technique was used. Controls of specificity for various peptides were studied with solid-phase absorption. Colchicine treatment was necessary to obtain positivity in many neu...
Burbach, J.Peter H. Wang, Xinchang van Ittersum, Marjanne
Published in
Biochemical and Biophysical Research Communications
Arginine-vasopressin and oxytocin, peptides which serve as putative precursors for neurotrophic fragments, were digested in the presence of the respective 14C-Tyr 2- and 14C-GlyNH 2 9-labeled nonapeptides with a purified synaptic membrane preparation of rat brain. In this preparation aminopeptidase activity predominates in the conversion of these p...
Vittet, Daniel Berta, Philippe Mathieu, Marie-Noelle Rondot, Anne Travo, Pierre Cantau, Bernard Chevillard, Claude
Published in
Biochemical and Biophysical Research Communications
Arginine vasopressin stimulated the accumulation of labeled inositol phosphate in cultured rat aortic myocytes prelabeled with tritiated myo-inositol. This accumulation was prevented by pretreating the myocytes with the phorbol ester PMA. The time-course and concentration-effect curves were similar for inositol phosphate formation in myocytes and c...
Ansseau, Marc Legros, Jean-Jacques Mormont, Christian Cerfontaine, Jean-Luc Papart, Patrick Geenen, Vincent Franck, Georges
peer reviewed / A 55 years patient with obsessive-compulsive disorder showed clear improvement during four weeks of treatment with intranasal oxytocin compared to four weeks of intranasal placebo. This improvement was concurrent with the development of severe memory disturbances, supporting the amnestic properties of the peptide. However, the patie...
Burbach, J.Peter H. Voorhuis, Theodora A.M. van Tol, Hubert H.M. Ivell, Richard
Published in
Biochemical and Biophysical Research Communications
Oxytocin mRNA was detected in the rat hypothalamus by in situ hybridization to a single stranded 35S-labelled DNA probe and the distribution of oxytocin mRNA-containing cell groups was studied at the macroscopic level. Specificity of hybridization was confirmed by comparison to vasopressin mRNA hybridization in parallel tissue sections. Cell groups...
Ivanisevic, Marina Behrens, Oliver Helmer, Hanns Demarest, Keith Fuchs, Anna-Riitta
Published in
American Journal of Obstetrics and Gynecology
Saturation analysis and competition experiments were performed to identify and characterize [ 3H]arginine vasopressin binding to human myometrium and decidua in late pregnancy. [ 3H]Arginine vasopressin bound with affinity similar to that of [ 3H]oxytocin to both tissues (dissociation constant 1 to 2 nmol/L). The concentration of [ 3H]arginine vaso...
