Safety aspects of novel foods
Metabolomics : quantification of intracellular metabolite dynamics
The rational improvement of microbial strains for the production of primary and secondary metabolites ('metabolic engineering') requires a quantitative understanding of microbial metabolism. A process by which this information can be derived from dynamic fermentation experiments is presented. By applying a substrate pulse to a substrate-limited, st...
Analysis of Highly Polar Compounds of Plant Origin: Combination of Hydrophilic Interaction Chromatography and Electrospr...
Published in Analytical Biochemistry
The primary goal of metabolomic analysis is the unbiased relative quantification of every metabolite in a biological system. A number of different metabolite-profiling techniques must be combined to make this possible. Here we report the separation and analysis of highly polar compounds in a proof of concept study. Compounds were separated and anal...
Can metabolomics be used for assessing nutritive-dependent human diseases?
Published in Phytochemistry Reviews
Metabolomic approaches are taking place within emerging technologies that share the focus to unravel unexpected effects in classical biological experiments, for example mutant/wild type comparisons. This review highlights the possibility of metabolomics going beyond this stage and comparing the onset and the pathogenesis of complex diseases, such a...
MetaGeneAlyse: analysis of integrated transcriptional and metabolite data
New techniques in sample preparation allow high throughput analysis of samples on the transcriptional as well as on the metabolic level. We present a service accessible via the web that allows the analysis of integrated data sets that combine gene-expression data and metabolic data. After uploading, data sets can be normalized, clustered by various...
A functional genomics approach toward the understanding of secondary metabolism in plant cells.
...Published in Proceedings of the National Academy of Sciences of the United States of America
Despite the tremendous importance of secondary metabolites for humans as for the plant itself, plant secondary metabolism remains poorly characterized. Here, we present an experimental approach, based on functional genomics, to facilitate gene discovery in plant secondary metabolism. Targeted metabolite analysis was combined with cDNA-amplified fra...
Secretion of secondary metabolites by ATP-binding cassette transporters in plant cell suspension cultures
Plant Physiology Vol.131 Nr.3, 1161 - 1164
Correlation between heat-shock protein induction and reduced metabolic condition in juvenile steelhead trout (Oncorhynch...
Published in Fish Physiology and Biochemistry
The metabolic condition, stress response, and growth rates of juvenile steelhead trout (Oncorhynchus mykiss) were assessed throughout a 10-week exposure to 15 and 20 °C, each at full and half food ration. Metabolic measurements included white muscle phosphocreatine (PCr), ATP, ADP and AMP, hepatic glycogen, and global metabolite profiling of muscle...
Connecting genes to metabolites by a systems biology approach.
Published in Proceedings of the National Academy of Sciences of the United States of America
Plant cell factories in the post-genomic era: new ways to produce designer secondary metabolites
Plants synthesize an extensive array of secondary metabolites, often with highly complex structures. Currently, most pharmaceutically important secondary metabolites are isolated from wild or cultivated plants because their chemical synthesis is not economically feasible. Biotechnological production in plant cell cultures is an attractive alternati...
