Bobrovskyy, Maksym Oh, So Young Missiakas, Dominique
Published in
The Journal of biological chemistry
Secretion systems utilize ATPase activity to facilitate the translocation of proteins into and across membranes. In bacteria, the universally conserved SecA ATPase binds a large repertoire of preproteins and interacts with the SecYEG translocon. In contrast, the type 7b secretion system (T7bSS) of Staphylococcus aureus supports the secretion of a r...
Lamm, Lorenz Righetto, Ricardo D Wietrzynski, Wojciech Pöge, Matthias Martinez-Sanchez, Antonio Peng, Tingying Engel, Benjamin D
Published in
Computer methods and programs in biomedicine
Cryo-electron tomography (cryo-ET) is an imaging technique that enables 3D visualization of the native cellular environment at sub-nanometer resolution, providing unpreceded insights into the molecular organization of cells. However, cryo-electron tomograms suffer from low signal-to-noise ratios and anisotropic resolution, which makes subsequent im...
Vasquez-Montes, Victor Tyagi, Vivek Sikorski, Eden Kyrychenko, Alexander Thévenin, Damien Tobias, Douglas Ladokhin, Alexey Freites, Juan
Cancerous tissues undergo extensive changes to their cellular environments that differentiate them from healthy tissues. These changes include changes in extracellular pH and Ca2+ concentrations, and the exposure of phosphatidylserine (PS) to the extracellular environment, which can modulate the interaction of peptides and proteins with the plasma ...
Li, Shuo
Published in
Acta biochimica et biophysica Sinica
Structure determination of membrane proteins has been a long-standing challenge to understand the molecular basis of life processes. Detergents are widely used to study the structure and function of membrane proteins by various experimental methods, and the application of membrane mimetics is also a prevalent trend in the field of cryo-EM analysis....
Puljung, Michael Haythorne, Elizabeth Rohm, Maria Vedovato, Natascia
Published in
Frontiers in Pharmacology
Vasilopoulou, Eleni Giannakopoulou, Artemis Kapsalis, Charalampos Michou, Myrsini Michoglou-Sergiou, Aristeidis Kolisis, Fragiskos N Skretas, Georgios
Published in
ACS synthetic biology
Escherichia coli is one of the most widely utilized hosts for recombinant protein production, including that of membrane proteins (MPs). We have recently engineered a specialized E. coli strain for enhanced recombinant MP production, termed SuptoxR. By appropriately co-expressing the effector gene rraA, SuptoxR can suppress the high toxicity, which...
Mehta, Ved Khanppnavar, Basavraj Schuster, Dina Kantarci, Ilayda Vercellino, Irene Kosturanova, Angela Iype, Tarun Stefanic, Sasa Picotti, Paola Korkhov, Volodymyr M
...
Published in
eLife
Mycobacterium tuberculosis adenylyl cyclase (AC) Rv1625c/Cya is an evolutionary ancestor of the mammalian membrane ACs and a model system for studies of their structure and function. Although the vital role of ACs in cellular signalling is well established, the function of their transmembrane (TM) regions remains unknown. Here, we describe the cryo...
Li, Qingxin Kang, Congbao
Published in
Frontiers in Cellular and Infection Microbiology
Dengue virus is an important pathogen affecting global population while no specific treatment is available against this virus. Effort has been made to develop inhibitors through targeting viral nonstructural proteins such as NS3 and NS5 with enzymatic activities. No potent inhibitors entering clinical studies have been developed so far due to many ...
Millrine, David Cummings, Thomas Matthews, Stephen P Peter, Joshua J Magnussen, Helge M Lange, Sven M Macartney, Thomas Lamoliatte, Frederic Knebel, Axel Kulathu, Yogesh
...
Published in
Cell reports
An essential first step in the post-translational modification of proteins with UFM1, UFMylation, is the proteolytic cleavage of pro-UFM1 to expose a C-terminal glycine. Of the two UFM1-specific proteases (UFSPs) identified in humans, only UFSP2 is reported to be active, since the annotated sequence of UFSP1 lacks critical catalytic residues. Nonet...
Skurska, Edyta Szulc, Bożena Maszczak-Seneczko, Dorota Wiktor, Maciej Wiertelak, Wojciech Makowiecka, Aleksandra Olczak, Mariusz
Published in
The Journal of biological chemistry
Mutations in the SLC35C1 gene encoding the Golgi GDP-fucose transporter are known to cause leukocyte adhesion deficiency II. However, improvement of fucosylation in leukocyte adhesion deficiency II patients treated with exogenous fucose suggests the existence of an SLC35C1-independent route of GDP-fucose transport, which remains a mystery. To inves...
