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In vitro immunization of human peripheral blood lymphocytes: establishment of B cell lines secreting IgM specific for ch...

Ichikawa, Akira Katakura, Yoshinori Teruya, Kiichiro Hashizume, Shuichi Shirahata, Sanetaka

In vitro immunization (IVI) techniques have a great potential in the production of human monoclonal antibodies (MAbs) against various antigens. An IVI method of human peripheral blood lymphocytes (PBL) has been developed with a human lung adenocarcinoma cell line in our laboratory. Although several cancer specific human MAbs were successfully gener...

Relationship between antibody productivity by activated human lymph node lymphocytes from lung cancer patients and lymph...

Yoshinari, Kaoru Arai, Kenji Matsumoto, Kunio Kimura, Hideki

Published in Cytotechnology

Regional lymph node lymphocytes from five patients with primary lung cancer were analyzed for subset composition, and exposed in vitro to the polyclonal human B cell mitogen Staphylococcus aureus Cowan I (SACI) or the murine B cell mitogen lipopolysaccharide (LPS) and then fused with mouse myeloma cells for investigation at the clonal level of thei...

Publication search with human monoclonal antibody as keyword

In vitro immunization of human peripheral blood lymphocytes: establishment of B cell lines secreting IgM specific for ch...

Relationship between antibody productivity by activated human lymph node lymphocytes from lung cancer patients and lymph...

Publication search
with human monoclonal antibody as keyword