Soni, Priyanka Yasuhara, Atsuhiro Takenaga, Toru Iwatsuki-Horimoto, Kiyoko Uraki, Ryuta Ito, Mutsumi Sasaki, Tadahiro Ikuta, Kazuyoshi Yamayoshi, Seiya Kawaoka, Yoshihiro
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Published in
The Journal of veterinary medical science
Influenza B virus has been known to infect humans and other animals, including seals. Vaccination efficacy varies across seasons. Human monoclonal antibodies (mAbs) can be useful for developing novel vaccines, guided by epitope analysis, and can be used therapeutically. Hybridoma technology has been used to make mAbs. Here we evaluated SPYMEG as a ...
Yamayoshi, Seiya Ito, Mutsumi Uraki, Ryuta Sasaki, Tadahiro Ikuta, Kazuyoshi Kawaoka, Yoshihiro
Published in
The Journal of infection
Broadly reactive human monoclonal antibodies against the HA stem of influenza A virus are being developed as therapeutic agents as well as to understand the epitopes that are essential for a universal influenza virus vaccine. We isolated and characterized two hetero-reactive human monoclonal antibodies from an H3N2 virus-infected human. These antib...
Romero Pastrana, Francisco Thompson, John M Heuker, Marjolein Hoekstra, Hedzer Dillen, Carly A Ortines, Roger V Ashbaugh, Alyssa G Pickett, Julie E Linssen, Matthijs D Bernthal, Nicholas M
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Published in
Virulence
Staphylococcus aureus infections are a major threat in healthcare, requiring adequate early-stage diagnosis and treatment. This calls for novel diagnostic tools that allow noninvasive in vivo detection of staphylococci. Here we performed a preclinical study to investigate a novel fully-human monoclonal antibody 1D9 that specifically targets the imm...
Romero Pastrana, Francisco Thompson, John M Heuker, Marjolein Hoekstra, Hedzer Dillen, Carly A Ortines, Roger V Ashbaugh, Alyssa G Pickett, Julie E Linssen, Matthijs D Bernthal, Nicholas M
...
Staphylococcus aureus infections are a major threat in healthcare, requiring adequate early-stage diagnosis and treatment. This calls for novel diagnostic tools that allow noninvasive in vivo detection of staphylococci. Here we performed a preclinical study to investigate a novel fully-human monoclonal antibody 1D9 that specifically targets the imm...
Yasuhara, Atsuhiro Yamayoshi, Seiya Ito, Mutsumi Kiso, Maki Yamada, Shinya Kawaoka, Yoshihiro
Published in
Frontiers in microbiology
The influenza A virus rapidly mutates to escape from antibodies. Here, we isolated and characterized three human monoclonal antibodies (mAbs) that neutralize A(H1N1)pdm09 viruses. Generation of escape mutant viruses suggested that these antibodies recognized conserved residues of the receptor-binding site (RBS) of hemagglutinin (HA) and that mutant...
Kim, Sangkyu Park, Insoo Park, Seung Gu Cho, Seulki Kim, Jin Hong Ipper, Nagesh S Choi, Sun Shim Lee, Eung Suk Hong, Hyo Jeong
Published in
Molecules and cells
We constructed a large naïve human Fab library (3 × 1010 colonies) from the lymphocytes of 809 human donors, assessed available diversities of the heavy-chain variable (VH) and κ light-chain variable (VK) domain repertoires, and validated the library by selecting Fabs against 10 therapeutically relevant antigens by phage display. We obtained a data...
Chen, Zhe Bao, Linlin Chen, Cong Zou, Tingting Xue, Ying Li, Fengdi Lv, Qi Gu, Songzhi Gao, Xiaopan Cui, Sheng
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Published in
The Journal of infectious diseases
Middle East respiratory syndrome coronavirus (MERS-CoV) infection in humans is highly lethal, with a fatality rate of 35%. New prophylactic and therapeutic strategies to combat human infections are urgently needed. We isolated a fully human neutralizing antibody, MCA1, from a human survivor. The antibody recognizes the receptor-binding domain of ME...
Smith, Heidi L. Lobikin, Maria Gao, Wenda Saia, Greg Wang, Yan Leney, Mark Molrine, Deborah C.
Background: Morbidity and mortality from Corynebacterium diphtheriae is reduced by prompt administration of equine-derived diphtheria anti-toxin (DAT), which is in short supply worldwide. MassBiologics has developed a human monoclonal antibody (S315) to diphtheria toxin to provide a safer alternative to DAT and address critical supply issues. S315 ...
Gibbs, John P Slatter, J Greg Egbuna, Ogo Geller, Michelle Hamilton, Lisa Dias, Clapton S Xu, Ren Y Johnson, Jessica Wasserman, Scott M Emery, Maurice G
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Published in
Journal of clinical pharmacology
Evolocumab binds PCSK9, increasing low-density lipoprotein cholesterol (LDL-C) receptors and lowering LDL-C. Target-mediated evolocumab elimination is attributable to PCSK9 binding. As circulating PCSK9 and LDL-C levels are primarily regulated by the liver, we compared evolocumab pharmacokinetics, pharmacodynamics, and safety in individuals with an...
Wang, Yan Kern, Aurelie Boatright, Naomi Schiller, Zachary Sadowski, Andrew Monir, Ejemel Souders, Colby Reimann, Keith A. Hu, Linden Klempner, Mark S.
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BACKGROUND: Tick transmission of Borrelia spirochetes to humans results in significant morbidity from Lyme disease worldwide. Serum concentrations of antibodies against outer surface protein A (OspA) were shown to correlate with protection from infection with Borrelia burgdorferi, the primary cause of Lyme disease in the United States. METHODS: Mic...
