Published in Plant Growth Regulation
A range of tissue culture conditions were tested to improve embryo culture frequency, and to develop an efficient plant regeneration system for triticale. Immature embryos (14–21 days post-anthesis) from two triticale genotypes (Hx87-139 and Tahara) were cultured on a commonly used Murashige and Skoog (MS) and on Lazzeri's (L1) basal medium with va...
Published in Plant Growth Regulation
A successful procedure was established for in vitro plant regeneration from callus derived from stem and leaf explants of Centella asiatica on semisolid modified Murashige and Skoog's [7] medium supplemented with 2.0 mg L3 kinetin and 4.0 mg L3 a-naphthaleneacetic acid. The rate of shoot-bud regeneration was the highest (42.8 and 54.3 shoots/cultur...
Published in Journal of Plant Biology
This study was designed to evaluate the effect of phytohormones on plant regeneration from epicotyl and hypocotyl explants of two groundnut (Arachis hypogaea) cultivars. Explants cultured on media with auxins and in combination with cytokinin produced high frequency of callus. After four weeks, callus from these cultures was transferred to medium w...
Published in In Vitro Cellular & Developmental Biology - Plant
Yellowish compact callus, induced from cowpea hypocotyls on Murashige and Skoog(MS) medium (1962) containing 0.2 mg/l(0.93 μM) kinetin and 0.4 mg/l (1.81 μM) 2,4-dichlorophenoxyacetic acid (2,4-D), was subcultured on MS medium containing cytokinin alone, auxin alone, or auxins plus cytokinins in order to determine the effect of cytokinins on root o...
Published in Plant Growth Regulation
Activated charcoal is commonly used in tissue culture media. Its addition to culture medium may promote or inhibit in vitro growth, depending on species and tissues used. The effects of activated charcoal may be attributed to establishing a darkened environment; adsorption of undesirable/inhibitory substances; adsorption of growth regulators and ot...
Published in Potato Research
Shoots, roots and callus were formed from tuber discs of potato, cultivar Désirée, when grown in vitro on the basal medium of Murashige & Skoog (1962) (MS) supplemented with 2,4-D and/or BAP. Callus was formed in MS medium with 1 mg l−1 BAP plus 0.5 mg l−1 2,4-D, callus and roots were formed in MS with 1 mg l−1 BAP plus more than 0.5 mg l−1 2,4-D a...
Published in Plant Cell, Tissue and Organ Culture
Rapid plant regeneration was achieved in callus cultures derived from leaf and stem explants of Plumbago zeylanica Linn. on MS basal medium supplemented with 4.44 μM 6-BA, 1.42 μM IAA and 3% (w/v) sucrose. The rate of shoot bud regeneration was positively correlated with the concentration of growth regulators in the nutrient media. The leaf explant...
Published in Experimental & Applied Acarology
Published in Plant Cell, Tissue and Organ Culture
Traditional propagation of lemon balm (Melissa officinalis L.) is inefficient for establishing a good quality clonal population. Results of the presented experiments outline an effective method for micropropagation of this species. Following culture initiation from shoots of field-grown plants on growth regulator free Murashige–Skoog medium, rapid ...
Published in Plant Cell, Tissue and Organ Culture
Jojoba (Simmondsia chinensis, (Link) Schneider) seedling explants were cultured on a modified Driver Kuniyuki medium, supplemented with various concentrations of 6-benzyladenine alone and in combination with silver nitrate. Shoot proliferation was successful at all the concentrations tested, with a maximum number of 15.2 shoots per original explant...
