David, J Gordon, J S Rutter, W J
Published in
Proceedings of the National Academy of Sciences of the United States of America
The replacement of thymidine by 5-bromodeoxyuridine in DNA leads to a greatly enhanced stability of chromatin from hepatoma tissue culture or embryonic rat pancreas, as measured by thermal chromatography on hydroxylapatite. The increased stability is directly correlated with the degree of bromodeoxyuridine substitution. On the other hand, the incor...
McCorkle, G M Leathers, T D Umbarger, H E
Published in
Proceedings of the National Academy of Sciences of the United States of America
We have determined the physical location of the ilvEDAC genes on the restriction cleavage map of the ilv region of Escherichia coli K-12 by two methods: (i) heteroduplex and endonuclease cleavage analysis of hybrid phages carrying genetically defined parts of the ilv cluster and (ii) complementation analysis and enzyme assays to determine ilv gene ...
Rowley, P T Ohlsson-Wilhelm, B Farley, B A Kosciolek, B
Published in
Proceedings of the National Academy of Sciences of the United States of America
A recent theory of the control of human fetal hemoglobin synthesis, based on studies in cultured adult marrow, proposes that the phenotypic expression of fetal hemoglobin is largely dependent on the level of differentiation of the parental stem cells; that is, the earlier the progenitor, the greater the ability of its progeny to express fetal hemog...
Wiedemann, L M Johnson, L F
Published in
Proceedings of the National Academy of Sciences of the United States of America
We have isolated a methotrexate (MTX)-resistant clone of mouse 3T6 cells, designated M50L3, which grows normally in the presence or absence of 50 muM MTX and produces a level of dihydrofolate reductase (DHFR; 5,6,7,8-tetrahydrofolate:NADP(+) oxidoreductase, EC 1.5.1.3) that is increased about 300-fold compared to the parental 3T6 cells. The cells r...
Bernstein, A Gamble, C Penrose, D Mak, T W
Published in
Proceedings of the National Academy of Sciences of the United States of America
The nature and distribution of sequences related to the murine erythroleukemia virus, Friend spleen focus-forming virus (SFFV), have been analyzed by using a radioactive cDNA probe specific for the SFFV genome (cDNA(sff)). From the proportion of high molecular weight viral [(32)P]RNA which hybridized to cDNA(sff), it was estimated that these sequen...
Pellicer, A Wagner, E F el-Kareh, A Dewey, M J Reuser, A J Silverstein, S Axel, R Mintz, B
Published in
Proceedings of the National Academy of Sciences of the United States of America
Teratocarcinoma (TCC) stem cells provide unique prospects for the introduction of specific genes into mice, by virtue of their dual capacity for propagation in vitro and for normal differentiation in embryos. In this study, we have demonstrated that foreign genes amenable to selection in culture can be transferred into the stem cells and expressed....
Scandalios, J G Chang, D Y McMillin, D E Tsaftaris, A Moll, R H
Published in
Proceedings of the National Academy of Sciences of the United States of America
Genetic and biochemical analyses suggest that the developmental program of catalase (H(2)O(2):H(2)O(2) oxidoreductase, EC 1.11.1.6) activity in maize scutella is controlled by a temporal regulatory gene (Car1) that is distinct from the structural genes thus far identified. Recombination data show that Car1 is located about 37 map units from the Cat...
Horinouchi, S Weisblum, B
Published in
Proceedings of the National Academy of Sciences of the United States of America
The nucleotide sequence of a gene in plasmid pE194 responsible for erythromycin-induced resistance, including regulation of the resistance phenotype, is reported. A DNA fragment from plasmid pE194, obtained by digestion with Taq I restriction endonuclease, was cloned in Bacillus subtilis by using pC194 as the plasmid cloning vector. Erythromycin-re...
Moriarty, A M Hoyer, B H Shih, J W Gerin, J L Hamer, D H
Published in
Proceedings of the National Academy of Sciences of the United States of America
We have constructed a simian virus 40 recombinant carrying a fragment of DNA from hepatitis B virus. Cultured monkey kidney cells infected with this recombinant produce hepatitis B surface antigen. The antigen is excreted into the culture medium as 22-nm particles with the same physical properties, antigenic composition, and constituent polypeptide...
Theologis, A Ray, P M
Published in
Proceedings of the National Academy of Sciences of the United States of America
Polyadenylylated mRNA from etiolated pea stem segments treated with or without 20 muM indoleacetic acid (IAA) for various periods of time was assayed by translating it in a wheat germ extract containing [(35)S]methionine and separating the translation products by two-dimensional gel electrophoresis. Within 2 hr IAA causes at least five mRNA sequenc...
