In this thesis natural and induced DNA sequence diversity in potato (Solanum tuberosum) for use in marker-trait analysis and potato breeding is assessed. The study addresses the challenges of reliable, high-throughput identification and genotyping of sequence variants in existing tetraploid potato cultivar panels using traditional Sanger sequencing...
Kroeskop is een al lang bekend verschijnsel in de rozenteelt waarbij geoculeerde rozen na ontspruiten van de oculatie dwerggroei en heksenbezemachtige groeiproblemen vertonen en een groot deel van de aangetaste planten sterft. Het pathogeen dat de aantasting veroorzaakt is een mysterie. Nieuwe detectietechnieken zoals de PCR-methode, DNA-sequentiea...
Onlangs is de DNA-sequentie van het genoom van Cladosporium fulvum bepaald. Het voornaamste doel daarvan is de identificatie en karakterisering van nieuwe effectors.
Lactobacillus plantarum is a lactic acid bacterium encountered in a variety of food and feed fermentations and as a natural inhabitant of human gastrointestinal tract. To survive in these niches and to maintain its capability, L. plantarum has to respond to numerous changing conditions and the cellular processes involved in these responses are freq...
In this thesis we describe interrenal hyperplasia in a teleost fish, the common carp, Cyprinus carpio L, caused by 17α-hydroxylase deficiency. Two homozygous, XX male inbred strains, “E5” and “E7”, consistently show 5-10 fold less plasma cortisol in response to net confinement compared to normal male and female carp strains. Both E5 and E7 fish hav...
In 2004 the chicken genome sequence and more than 2.8 million single nucleotide polymorphisms (SNPs) were reported. This information greatly enhanced the ability of poultry scientists to understand chicken biology, especially with respect to identification of quantitative trait loci (QTL) and genes that control simple and complex traits. To validat...
Allergic diseases, such as hay fever and food allergy, affect a substantial part of the population in westernized countries. Pollen of the European white birch (Betula pendula) is a considerable cause of hay fever (seasonal allergic rhinitis) in northern and central Europe. The major birch pollen allergen is Bet ν 1, which is the conventional aller...
Large amounts of genome sequence data are available and much more will become available in the near future. A DNA sequence alone has, however, limited use. Genome annotation is required to assign biological interpretation to the DNA sequence. This thesis describes a variety of research topics for bioinformatics in the context of genome annotation. ...
Tomato early blight (EB) caused by the fungus Alternaria solani is a field disease with a worldwide distribution, including Indonesia. The disease is currently controlled using frequent applications of fungicides. The use of resistant cultivar would be an attractive way to reduce fungicide application. The aims of the research are to support breedi...
The relationship between the structure of genes and their expression is a relatively new aspect of genome organization and regulation. With more genome sequences and expression data becoming available, bioinformatics approaches can help the further elucidation of the relationships between gene structure and gene expression. This will contribute to ...
