Prendergast, Laura Reinberg, Danny
Published in
Genes & development
Major advances in the chromatin and epigenetics fields have uncovered the importance of core histones, histone variants and their post-translational modifications (PTMs) in modulating chromatin structure. However, an acutely understudied related feature of chromatin structure is the role of linker histone H1. Previous assumptions of the functional ...
Desideri, Fabio Cipriano, Andrea Petrezselyova, Silvia Buonaiuto, Giulia Santini, Tiziana Kasparek, Petr Prochazka, Jan Janson, Giacomo Paiardini, Alessandro Calicchio, Alessandro
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Published in
Cell Reports
Desideri et al. study the role of the chromatin-retained isoform of Charme , a lncRNA that regulates myogenesis. They find that a MATR3/PTBP1/ pCharme assembly drives the retention and the function of the lncRNA. CRISPR-Cas9 deletion of pCharme intron-1 leads to the transcript delocalization and to heart defects in vivo .
Pachva, Manideep C. Kisselev, Alexei F. Matkarimov, Bakhyt T. Saparbaev, Murat Groisman, Regina
Published in
Frontiers in Cell and Developmental Biology
The nucleosome is a stretch of DNA wrapped around a histone octamer. Electrostatic interactions and hydrogen bonds between histones and DNA are vital for the stable organization of nucleosome core particles, and for the folding of chromatin into more compact structures, which regulate gene expression via controlled access to DNA. As a drawback of t...
Perner, Florian Armstrong, Scott A.
Published in
Cells
The aberrant function of chromatin regulatory networks (epigenetics) is a hallmark of cancer promoting oncogenic gene expression. A growing body of evidence suggests that the disruption of specific chromatin-associated protein complexes has therapeutic potential in malignant conditions, particularly those that are driven by aberrant chromatin modif...
Trovato, Matteo Patil, Vibha Gehre, Maja Noh, Kyung Min
Published in
Cells
The systematic mutation of histone 3 (H3) genes in model organisms has proven to be a valuable tool to distinguish the functional role of histone residues. No system exists in mammalian cells to directly manipulate canonical histone H3 due to a large number of clustered and multi-loci histone genes. Over the years, oncogenic histone mutations in a ...
Eckersley-Maslin, Mélanie A.
Published in
Biochemical Society Transactions
The concept of cellular plasticity is particularly apt in early embryonic development, where there is a tug-of-war between the stability and flexibility of cell identity. This balance is controlled in part through epigenetic mechanisms. Epigenetic plasticity dictates how malleable cells are to change by adjusting the potential to initiate new trans...
Hollin, Thomas Le Roch, Karine G.
Published in
Frontiers in Cellular and Infection Microbiology
Over the past decade, we have witnessed significant progresses in understanding gene regulation in Apicomplexa including the human malaria parasite, Plasmodium falciparum . This parasite possesses the ability to convert in multiple stages in various hosts, cell types, and environments. Recent findings indicate that P. falciparum is talented at usin...
Panda, Amitesh Zylicz, Jan J Pasque, Vincent
Published in
Cells
Dosage compensation between the sexes results in one X chromosome being inactivated during female mammalian development. Chromosome-wide transcriptional silencing from the inactive X chromosome (Xi) in mammalian cells is erased in a process termed X-chromosome reactivation (XCR), which has emerged as a paradigm for studying the reversal of chromati...
Hussain, Imran Deb, Paromita Chini, Avisankar Obaid, Monira Bhan, Arunoday Ansari, Khairul I. Mishra, Bibhu P. Bobzean, Samara A. Udden, S. M. Nashir Alluri, Prasanna G.
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Published in
Frontiers in Genetics
HOXA5 is a homeobox-containing gene associated with the development of the lung, gastrointestinal tract, and vertebrae. Here, we investigate potential roles and the gene regulatory mechanism in HOXA5 in breast cancer cells. Our studies demonstrate that HOXA5 expression is elevated in breast cancer tissues and in estrogen receptor (ER)-positive brea...
Liu, Xingyu Zhang, Ying Wen, Zhihui Hao, Yan Banks, Charles A S Lange, Jeffrey J Slaughter, Brian D Unruh, Jay R Florens, Laurence Abmayr, Susan M
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Published in
Proceedings of the National Academy of Sciences of the United States of America
Streamlined characterization of protein complexes remains a challenge for the study of protein interaction networks. Here we describe serial capture affinity purification (SCAP), in which two separate proteins are tagged with either the HaloTag or the SNAP-tag, permitting a multistep affinity enrichment of specific protein complexes. The multifunct...