Published in Cells
The urokinase (uPA) receptor (uPAR) plays a key role in cell migration. We previously showed that uPAR-negative HEK-293 cells efficiently migrate toward serum but, after uPAR ectopic expression, migrate only in a uPAR-dependent manner. In fact, migration of uPAR-transfected HEK-293 (uPAR-293) cells is impaired by anti-uPAR antibodies, without recov...
Published in Cell reports
The activation of G-protein-coupled receptors (GPCRs) leads to the activation of mTORC2 in cell migration and metabolism. However, the mechanism that links GPCRs to mTORC2 remains unknown. Here, using Dictyostelium cells, we show that GPCR-mediated chemotactic stimulation induces hetero-oligomerization of phosphorylated GDP-bound Rho GTPase and GTP...
The dynamic organization of actin cytoskeleton meshworks relies on multiple actinbinding proteins endowed with distinct actin-remodeling activities. Filamin A is a large multi-domain scaffolding protein that cross-links actin filaments with orthogonal orientation in response to various stimuli. As such it plays key roles in the modulation of cell s...
Published in The Journal of pathology
Distal cholangiocarcinoma (dCCA) is a biliary tract cancer with a dismal prognosis and is often preceded by biliary intraepithelial neoplasia (BilIN), representing the most common biliary non-invasive precursor lesion. BilIN are histologically well defined but have not so far been characterised systematically at the molecular level. The aim of this...
Published in Journal of cellular biochemistry
This study was intended to clarify the potential of applying the long-chain noncoding RNA H19/miR-29a axis in keloid treatment by elucidating its correlation with the activity of fibroblasts. In this study, 80 keloid tissues, 63 normal fibrous tissues, and 91 normal skin tissues were collected in advance, and concurrently, fibroblasts separated fro...
Published in Sheng wu gong cheng xue bao = Chinese journal of biotechnology
肾脏是人体重要器官，肾脏发育对肾脏的形成和功能至关重要，其中后肾间充质细胞 (Metanephric mesenchyme，MM) 间质-上皮转化 (Mesenchymal-epithelial transition，MET) 是肾单位形成的关键环节。qRT-PCR和Western blotting 实验检测蛋白质磷酸酶3 催化亚基α (Protein phosphatase 3 catalytic subunit alpha，PPP3CA) 在不同状态MM 细胞株mK3、mK4 中的表达谱及对MET 标志蛋白调控作用；采用慢病毒包装方式构建稳定敲低PPP3CA 的mK4 细胞株；采用CCK-8、EdU 实验、细胞划痕实验、流式细胞技术分别检测PPP3CA 对上皮样后肾间充质细胞株mK4 细...
Published in Open Medicine
Background and aim This investigation was aimed at disclosing whether SRPX2 affected pancreatic cancer (PC) chemoresistance by regulating PI3K/Akt/mTOR signaling. Methods Totally 243 PC patients were recruited, and they were incorporated into partial remission (PR) group, stable disease (SD) group and progressive disease (PD) group in accordance wi...
Published in OncoTargets and therapy
Background Many studies found that VPS53, one of the subunits of the golgi-associated retrograde protein (GARP) complexes, was aberrantly expressed in human diseases. Aim This study investigated the functions and molecular mechanisms of VPS53 in colorectal cancer (CRC). Methods Expression and correlation of Beclin 1 and VPS53 were analyzed by RT-qP...
Published in International Journal of Molecular Sciences
The receptor for advanced glycation end products (RAGE) contributes to many cellular aspects of pancreatic cancer including cell proliferation, migration, and survival. Studies have shown that RAGE activation by its ligands promotes pancreatic tumor growth by stimulating both cell proliferation and migration. In this study, we investigated the effe...
Published in eLife
Immune cells known as cytotoxic T lymphocytes, or CTLs for short, move around the body searching for infected or damaged cells that may cause harm. Once these specialised killer cells identify a target, they launch an attack, removing the harmful cell from the body. CTLs can also recognise and eliminate cancer cells, and can be infused into cancer ...
