AbuSamra, Dina B Al-Kilani, Alia Hamdan, Samir M Sakashita, Kosuke Gadhoum, Samah Z Merzaban, Jasmeen S
Published in
The Journal of biological chemistry
Selectins (E-, P-, and L-selectins) interact with glycoprotein ligands to mediate the essential tethering/rolling step in cell transport and delivery that captures migrating cells from the circulating flow. In this work, we developed a real time immunoprecipitation assay on a surface plasmon resonance chip that captures native glycoforms of two wel...
Koch, Britta Meyer, Anne K Helbig, Linda Harazim, Stefan M Storch, Alexander Sanchez, Samuel Schmidt, Oliver G
Published in
Nano letters
We employ glass microtube structures fabricated by rolled-up nanotechnology to infer the influence of scaffold dimensionality and cell confinement on neural stem cell (NSC) migration. Thereby, we observe a pronounced morphology change that marks a reversible mesenchymal to amoeboid migration mode transition. Space restrictions preset by the diamete...
Ran, Qi-Shan Yu, Yun-Hu Fu, Xiao-Hong Wen, Yuan-Chao
Published in
Neural regeneration research
The Notch signaling pathway plays a key role in angiogenesis and endothelial cell formation, but it remains unclear whether it is involved in vascular repair by endothelial progenitor cells after traumatic brain injury. Therefore, in the present study, we controlled the Notch signaling pathway using overexpression and knockdown constructs. Activati...
Calarco, A Di Salle, A Tammaro, L De Luca, I Mucerino, S Petillo, O Riccitiello, F Vittoria, V Peluso, G
Published in
Journal of dental research
Fluoride-releasing restorative dental materials can be beneficial to remineralize dentin and help prevent secondary caries. However, the effects of fluoride release from dental materials on the activity of dental pulp stem cells are not known. Here we investigate whether different fluoride release kinetics from dental resins supplemented with modif...
Verma, Pushpa Cohen, Stephen M
Published in
eLife
Formation of the Drosophila adult abdomen involves a process of tissue replacement in which larval epidermal cells are replaced by adult cells. The progenitors of the adult epidermis are specified during embryogenesis and, unlike the imaginal discs that make up the thoracic and head segments, they remain quiescent during larval development. During ...
Vafai, Nicholas Lowry, Troy W. Wilson, Korey A. Davidson, Michael W. Lenhert, Steven
Published in
Nanofabrication
Lipid multilayer microarrays are a promising approach to miniaturize laboratory procedures by taking advantage of the microscopic compartmentalization capabilities of lipids. Here, we demonstrate a new method to pattern lipid multilayers on surfaces based on solvent evaporation along the edge where a stencil contacts a surface called evaporative ed...
Schultz, Kelly M Kyburz, Kyle A Anseth, Kristi S
Published in
Proceedings of the National Academy of Sciences of the United States of America
Biomaterials that mimic aspects of the extracellular matrix by presenting a 3D microenvironment that cells can locally degrade and remodel are finding increased applications as wound-healing matrices, tissue engineering scaffolds, and even substrates for stem cell expansion. In vivo, cells do not simply reside in a static microenvironment, but inst...
Zanetti, Adriana Affatato, Roberta Centritto, Floriana Fratelli, Maddalena Kurosaki, Mami Barzago, Maria Monica Bolis, Marco Terao, Mineko Garattini, Enrico Paroni, Gabriela
...
Published in
The Journal of biological chemistry
All-trans-retinoic acid (ATRA) is a natural compound proposed for the treatment/chemoprevention of breast cancer. Increasing evidence indicates that aberrant regulation of epithelial-to-mesenchymal transition (EMT) is a determinant of the cancer cell invasive and metastatic behavior. The effects of ATRA on EMT are largely unknown. In HER2-positive ...
Hopper, Niina Henson, Frances Brooks, Roger Ali, Erden Rushton, Neil Wardale, Robert
Introduction: A major problem in cartilage repair is the lack of chondrogenic cells migrating from healthy tissue into defects. Cartilage is essentially avascular and therefore its healing is not considered to involve mononuclear cells. Peripheral blood derived mononuclear cells (PBMC) offer a readily available autologous cell source for clinical u...
Choi, Youngsil Kwon, Mi-Jung Lim, Yangmi Yun, Ji-Hye Lee, Weontae Oh, Eok-Soo
Published in
The Journal of biological chemistry
Syndecans, a family of transmembrane heparansulfate proteoglycans, are known to interact through their transmembrane domains to form non-covalently linked homodimers, a process essential for their individual functions. Because all syndecan transmembrane domains are highly conserved and thus might mediate interactions between different members of th...
