Nevo, Z Dorfman, A
Published in
Proceedings of the National Academy of Sciences of the United States of America
Chondrocytes in suspension culture preserve their differentiated state, as expressed by a high rate of chondromucoprotein synthesis. Purified chondromuco-protein added to a suspension of cultured embryonic epiphyseal cartilage cells stimulates the rate of synthesis of chondromucoprotein at concentrations up to 2 mg/ml. The stimulation involved synt...
Dudman, Nicholas P.B. Hicks, Christine Wilcken, David E.L.
Published in
Analytical Biochemistry
We assessed the strength of attachment of cultured human vascular endothelial cells to tissue culture plastic by controlled sluicing of cells, grown on multiwell plates, with isotonic saline using a specially designed nozzle attached to a reciprocating pump. Cells which were not dislodged were trypsinized and counted. The percentage of cells dislod...
Hwan Mook Kim Sang Bae Han Moon Soon Kim Jong Seong Kang Goo Taeg Oh Dong Ho Hong
The fixation procedures in sulforhodamine B (SRB) assay for human leukemia cells were modified to produce more reliable results. It was found that the concentration of the fixative agent, trichloroacetic acid (TCA), was critical in the selective fixation of cellular protein. While a TCA solution of 80% fixed both cells and serum proteins, a 50% sol...
kim, hwan mook han, sang bae kim, moon soon kang, jong seong taeg, goo hong, dong
The fixation procedures in sulforhodamine B (SRB) assay for human leukemia cells were modified to produce more reliable results. It was found that the concentration of the fixative agent, trichloroacetic acid (TCA), was critical in the selective fixation of cellular protein. While a TCA solution of 80% fixed both cells and serum proteins, a 50% sol...
Hu, G F Riordan, J F Vallee, B L
Published in
Proceedings of the National Academy of Sciences of the United States of America
Angiogenin stimulates both [3H]thymidine incorporation and proliferation of human endothelial cells in sparse cultures. Under these conditions, a 170-kDa cell surface protein can be detected that binds angiogenin specifically. Angiogenin-stimulated cell growth is concentration-dependent and is completely inhibited by an anti-angiogenin monoclonal a...
Kobayashi, Hiroyuki Takemura, Yuzuru Holland, James F. Ohnuma, Takao
Published in
Biochemical Pharmacology
Vincristine (VCR) is an active agent in the treatment of acute lymphoblastic leukemia (ALL). We evaluated the relationship between the cytotoxic activity of VCR and the degree of VCR saturation of cellular drug binding sites, using the MOLT-3 ALL cell line. When MOLT-3 cells at a density of 1 × 10 6 or 1 × 10 8 cells/mL of pH-controlled medium were...
Tamaki, Masashi McDonald, Warren Del Maestro, Rolando F.
Published in
Journal of Neuro-Oncology
We have examined the influence of basic fibroblast growth factor (FGF-2) and vascular endothelial growth factor (VEGF) on the release of collagenase type IV activity and the production of extracellular matrix (ECM) molecules using C6 astrocytoma cells in monolayer culture. Collagenase type IV activity was significantly increased in a dose dependent...
Georgakoudi, I Keng, P C Foster, T H
Published in
British journal of cancer
We have studied the effects of hypoxia on aminolaevulinic acid (ALA)-induced protoporphyrin IX (PpIX) synthesis in EMT6 monolayer cultures characterized by different cell densities and proliferation rates. Specifically, after ALA incubation under hypoxic or normoxic conditions, we detected spectrofluorometrically the PpIX content of the following p...
Qiao, Liang Farrell, Geoffrey C.
Published in
In Vitro Cellular & Developmental Biology - Animal
The rates of spontaneous cell detachment, cell viability, and apoptosis in primary cultures of rat hepatocytes plated at high and low density were compared. Apoptosis was frequent in detached cells, and the rates of cell detachment and apoptosis were greater in high-density than in low-density cultures. Among attached cells, more cells had condense...
Chang, Kyung Hwa Park, Chang-Ho Park, Jong Hwa Chung, In Sik
Published in
Biotechnology Techniques
The human rotavirus titer was optimal at an infection cell density of about 4–8 × 104 cells cm−2 in monkey kidney cell cultures. The highest viral titers (3.8 × 107 TCID50 ml−1 and 3.7 × 107 TCID50 ml−1) were obtained at an multiplicity of infection of 0.05 in well plate and T-flask, respectively.
