Rehemtulla, A Hamilton, A C Taneja, N Fridman, J Juan, T S Maybaum, J Chinnaiyan, A
Published in
Neoplasia (New York, N.Y.)
Bcl-2 and Bcl-X(L) belong to a family of proteins overexpressed in a variety of human cancers which inhibit apoptosis in response to a number of stimuli including chemotherapeutic agents and ionizing radiation. To better understand the role of these polypeptides in modulating the response of cancer cells to ionizing radiation we used cell lines tha...
Krebs, J F Armstrong, R C Srinivasan, A Aja, T Wong, A M Aboy, A Sayers, R Pham, B Vu, T Hoang, K
...
Published in
The Journal of cell biology
The mechanism by which membrane-bound Bcl-2 inhibits the activation of cytoplasmic procaspases is unknown. Here we characterize an intracellular, membrane-associated form of procaspase-3 whose activation is controlled by Bcl-2. Heavy membranes isolated from control cells contained a spontaneously activatable caspase-3 zymogen. In contrast, in Bcl-2...
Susin, S A Lorenzo, H K Zamzami, N Marzo, I Brenner, C Larochette, N Prévost, M C Alzari, P M Kroemer, G
Published in
The Journal of experimental medicine
The barrier function of mitochondrial membranes is perturbed early during the apoptotic process. Here we show that the mitochondria contain a caspase-like enzymatic activity cleaving the caspase substrate Z-VAD.afc, in addition to three biological activities previously suggested to participate in the apoptotic process: (a) cytochrome c; (b) an apop...
Moulder, K L Onodera, O Burke, J R Strittmatter, W J Johnson, E M Jr
Published in
The Journal of neuroscience : the official journal of the Society for Neuroscience
Recent evidence suggests that, in huntingtin and many other proteins, polyglutamine repeats are a toxic stimulus in neurodegenerative diseases. To investigate the mechanism by which these repeats may be toxic, we transfected primary rat cerebellar granule neurons with polyglutamine-green fluorescent protein (GFP) fusion constructs containing 19 (Q1...
MacDonald, G Shi, L Vande Velde, C Lieberman, J Greenberg, A H
Published in
The Journal of experimental medicine
Granzyme B (GraB) is required for the efficient activation of apoptosis by cytotoxic T lymphocytes and natural killer cells. We find that GraB and perforin induce severe mitochondrial perturbation as evidenced by the release of cytochrome c into the cytosol and suppression of transmembrane potential (Deltapsi). The earliest mitochondrial event was ...
Leist, Marcel Single, Barbara Naumann, Heike Fava, Eugenio Simon, Bernadett Kühnle, Simone Nicotera, Pierluigi
Published in
Biochemical and Biophysical Research Communications
The endogenous mediator nitric oxide (NO) blocked apoptosis of Jurkat cells elicited by staurosporine, anti-CD95 or chemotherapeutics, and switched death to necrosis. The switch in the mode of cell death was dependent on the ATP loss elicited by NO. This affected two distinct steps of the apoptotic cascade. First, the release of cytochrome cfrom mi...
Lemaire, Christophe Andréau, Karine Souvannavong, Vongthip Adam, Arlette
Published in
Biochemical Pharmacology
Cycloheximide (CHX) is known to stimulate or to prevent apoptosis, according to the cell type used. We found that CHX, in a dose-dependent way, exerted the two opposite effects in B lymphocytes. CHX high (2.5 μg/mL) inhibited protein synthesis (>90%) and greatly increased B cell apoptosis but failed to prevent apoptosis induction by dexamethasone (...
Granville, D J Jiang, H An, M T Levy, J G McManus, B M Hunt, D W
Published in
British journal of cancer
Treatment with the photosensitizer benzoporphyrin derivative monoacid ring A (BPD-MA, verteporfin) followed by irradiation with visible light induces apoptosis in human acute myelogenous leukaemia HL-60 cells. Photoactivation of BPD-MA induces procaspase 3 (CPP32/Yama/apopain) and procaspase 6 (Mch2) cleavage into their proteolytically active subun...
Ferrari, D. Los, Marek Jan Bauer, M. K. A. Vandenabeele, P. Wesselborg, Sebastian Schulze-Osthoff, K.
Myeloic cells express a peculiar surface receptor for extracellular ATP, called the P2Z/P2X(7) purinoreceptor, which is involved in cell death signalling. Here, we investigated the role of caspases, a family of proteases implicated in apoptosis and the cytokine secretion. We observed that extracellular ATP induced the activation of multiple caspase...
Fadeel, Bengt Gleiss, Bettina Högstrand, Kari Chandra, Joya Wiedmer, Therese Sims, Peter J. Henter, Jan-Inge Orrenius, Sten Samali, Afshin
Published in
Biochemical and Biophysical Research Communications
Phosphatidylserine (PS) exposure on the surface of cells has been considered a characteristic feature of apoptosis. However, we demonstrate herein that externalization of PS occurs in a cell-type-specific, albeit caspase-dependent, manner. Moreover, we could find no correlation in six different cell lines between the level of expression of the phos...
