Knall, C Worthen, G S Johnson, G L
Published in
Proceedings of the National Academy of Sciences of the United States of America
Chemoattractants and chemokines, such as interleukin 8 (IL-8), are defined by their ability to induce directed cell migration of responsive cells. The signal transduction pathway(s) leading to cell migration remain ill defined. We demonstrate that phosphatidylinositol-3-kinase (PI3K) activity, as determined by inhibition using wortmannin and LY2940...
Maroney, A C Glicksman, M A Basma, A N Walton, K M Knight, E Jr Murphy, C A Bartlett, B A Finn, J P Angeles, T Matsuda, Y
...
Published in
The Journal of neuroscience : the official journal of the Society for Neuroscience
Neurons undergoing apoptosis can be rescued by trophic factors that simultaneously increase the activity of extracellular signal-regulated kinase (ERK) and decrease c-Jun N-terminal kinase (JNK) and p38. We identified a molecule, CEP-1347 (KT7515), that rescues motoneurons undergoing apoptosis and investigated its effect on ERK1 and JNK1 activity. ...
Zhang, P. Hogan, E. L. Bhat, N. R.
Published in
Neurochemical Research
Recently, we reported on the activation of c-Jun N-terminal kinase (JNK) in primary glial cells noting certain differences in the patterns of kinase activation in astrocytes and oligodendrocytes (Zhang et al., J Neurosci Res 46:114–121;1996). In this extended study, we have examined the activation and expression levels of JNK1 and JNK2 isoforms in ...
Lee, YH Kim, SY Kim, JR Kim, KY Kim, MJ Ryu, SH Suh, PG Kim, JH
Ultraviolet-C (UVC) irradiation induces DNA damage and UVC-irradiated cells undergo cell growth arrest to repair the damaged DNA or the induction of apoptosis to prevent the risk of neoplastic transformation. Phospholipase C-gamma 1 (PLC-gamma 1) is a mediator of growth factor induced-signal cascade, catalyzing the hydrolysis of phosphatidyl 4,5-bi...
Lee, YH Kim, SY Kim, JR Kim, KY Kim, MJ Ryu, SH Suh, Pann-Ghill Kim, JH
Ultraviolet-C (UVC) irradiation induces DNA damage and UVC-irradiated cells undergo cell growth arrest to repair the damaged DNA or the induction of apoptosis to prevent the risk of neoplastic transformation. Phospholipase C-??1 (PLC-??1) is a mediator of growth factor induced-signal cascade, catalyzing the hydrolysis of phosphatidyl 4,5-bisphospha...
Wong, C.K. Zhang, J.P. Lam, C.W.K. Ho, C.Y. Hjelm, N.M.
Published in
International Archives of Allergy and Immunology
Background: It has been shown that the inhibition of eosinophilic apoptosis is an important mechanism for the development of blood and tissue eosinophilia in allergic diseases. Considerable attention has recently been focused on the role played by different intracellular kinase cascades in the control of apoptosis. In the present study, we investig...
Park, Raekil Kim, Myung-Sunny So, Hong-Seob Jung, Byung-Hak Moon, Sun-Rock Chung, Sang-Young Ko, Chang-Bo Kim, Bok-Ryang Chung, Hun-Taeg
Published in
Biochemical Pharmacology
Extracts of mistletoe (Viscum album var. coloratum) have been used for several decades as an anticancer immunomodulating agent in clinical fields. However, the mechanism by which the plant extracts kill tumor cells has remained elusive. We investigated the direct effects of beta-galactoside- and N-acetyl-d-galactosamine-specific mistletoe lectin II...
Arthur, John M. Lawrence, Martha S. Payne, Cameron R. Rane, Madhavi J. McLeish, Kenneth R.
Published in
Biochemical and Biophysical Research Communications
The calcium-sensing receptor (CaR) stimulates ERK1 in rat fibroblasts, but its effect on other MAP kinases is not known. We used a model of renal distal tubule, the MDCK cell, to determine the effects of CaR stimulation on Jun kinase (JNK) activity. Stimulation of the CaR with 5 mM Ca 2+ resulted in a time-dependent increase in JNK activity. Activa...
Kitamura, Masanori Ishikawa, Yoshihisa Konta, Tsuneo
Published in
Nephron Experimental Nephrology
Ex vivo incubation of isolated glomeruli has been used for many years to investigate biochemical properties of glomeruli. However, little has been understood about whether and how isolation and explanation per se affect the structure and function of the glomerulus. We recently reported that ex vivo incubation of isolated normal glomeruli results in...
Cheng, Ji-Zhong Singhal, Sharad S. Sharma, Abha Saini, Manjit Yang, Yusong Awasthi, Sanjay Zimniak, Piotr Awasthi, Yogesh C.
Published in
Archives of Biochemistry and Biophysics
The mammalian alpha-class glutathione S-transferase (GST) isozymes mGSTA4-4, rGSTA4-4, and hGSTA4-4 are known to utilize 4-hydroxynonenal (4HNE) as a preferred substrate. During the present studies, we have examined the effect of transfecting human myeloid HL-60 cells with mGSTA4, on 4-HNE-induced apoptosis and the associated signaling mechanisms. ...
