Accelerating Enzymatic Catalysis Using Vortex Fluidics
Accelerating Enzymatic Catalysis Using Vortex Fluidics
Discovery, Molecular Mechanisms, and Industrial Applications of Cold-Active Enzymes
Published in Frontiers in Microbiology
Cold-active enzymes constitute an attractive resource for biotechnological applications. Their high catalytic activity at temperatures below 25°C makes them excellent biocatalysts that eliminate the need of heating processes hampering the quality, sustainability, and cost-effectiveness of industrial production. Here we provide a review of the isola...
Biocatalysis and Biomass Conversion in Alternative Reaction Media.
Published in Chemistry (Weinheim an der Bergstrasse, Germany)
In this Minireview, the state of the art in the use of ionic liquids (ILs) and deep eutectic solvents (DESs) as alternative reaction media for biocatalytic processes and biomass conversion is presented. Initial, proof-of-concept studies, more than a decade ago, involved first-generation ILs based on dialkylimidazolium cations and non-coordinating a...
Enzymatic network for production of ether amines from alcohols.
...Published in Biotechnology and bioengineering
We constructed an enzymatic network composed of three different enzymes for the synthesis of valuable ether amines. The enzymatic reactions are interconnected to catalyze the oxidation and subsequent transamination of the substrate and to provide cofactor recycling. This allows production of the desired ether amines from the corresponding ether alc...
Engineering of alanine dehydrogenase from Bacillus subtilis for novel cofactor specificity.
Published in Biotechnology and applied biochemistry
The l-alanine dehydrogenase of Bacillus subtilis (BasAlaDH), which is strictly dependent on NADH as redox cofactor, efficiently catalyzes the reductive amination of pyruvate to l-alanine using ammonia as amino group donor. To enable application of BasAlaDH as regenerating enzyme in coupled reactions with NADPH-dependent alcohol dehydrogenases, we a...
A New Generation of Smart Amine Donors for Transaminase-Mediated Biotransformations.
Published in Chemistry (Weinheim an der Bergstrasse, Germany)
The application of ω-transaminase biocatalysts for the synthesis of optically pure chiral amines presents a number of challenges, including difficulties associated with displacing the challenging reaction equilibria. Herein, we report a highly effective approach using low equivalents of the new diamine donor, cadaverine, which enables high conversi...
Carbon Flux Trapping: Highly Efficient Production of Polymer-Grade d-Lactic Acid with a Thermophilic d-Lactate Dehydroge...
Published in Chembiochem : a European journal of chemical biology
High production of polymer-grade d-lactic acid is urgently required, particularly for the synthesis of polylactic acid. High-temperature fermentation has multiple advantages, such as lower equipment requirement and energy consumption, which are essential for lowering operating costs. We identified and introduced a unique d-lactate dehydrogenase int...
Self-Sufficient Formaldehyde-to-Methanol Conversion by Organometallic Formaldehyde Dismutase Mimic.
Published in Chemistry (Weinheim an der Bergstrasse, Germany)
The catalytic networks of methylotrophic organisms, featuring redox enzymes for the activation of one-carbon moieties, can serve as great inspiration in the development of novel homogeneously catalyzed pathways for the interconversion of C1 molecules at ambient conditions. An imidazolium-tagged arene-ruthenium complex was identified as an effective...
The Oxygen Dilemma: A Severe Challenge for the Application of Monooxygenases?
Published in Chembiochem : a European journal of chemical biology
Monooxygenases are promising catalysts because they in principle enable the organic chemist to perform highly selective oxyfunctionalisation reactions that are otherwise difficult to achieve. For this, monooxygenases require reducing equivalents, to allow reductive activation of molecular oxygen at the enzymes' active sites. However, these reducing...
