Valasques Junior, Gildomar Lima dos Santos, Jener David Gonçalves Chaves, Pedro Felipe Pereira Cordeiro, Lucimara Mach Côrtes de Jesus, Cleisiane Lima de Lima, Flávia Oliveira Boffo, Elisangela Fabiana de Assis, Sandra Aparecida
Published in
3 Biotech
Pseudozyma sp. are yeasts that are commercially important due to their production of glycolipid biosurfactants, squalene, itaconic acid, and exopolysaccharide. The search for other analgesia inducing drugs, such as opiates and non-steroidal anti-inflammatory drugs (NSAIDs), as alternatives is beneficial. In this study, the antinociceptive and anti-...
Glingston, Sahaya Rajpoot, Jitika Deori, Nayan Moni Deb, Rachayeeta Kumar, Sachin Nagotu, Shirisha
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3 Biotech
Newcastle disease virus is a member of family Paramyxoviridae that infects chicken. Its genome comprises ~15.2 kb negative-sense RNA that encodes six major proteins. The virus encodes various proteins; among all, nucleocapsid (NP) and matrix (M) help in virus replication and its budding from the host cells, respectively. In this study, we investiga...
Hirono-Hara, Yoko Yui, Miyuu Hara, Kiyotaka Y.
Published in
AMB Express
Transglutaminase (TG) catalyzes the formation of cross-links between proteins. TG from Streptoverticillium mobaraense (SmTG) is used widely in food, cosmetic, biomaterial and medical industries. SmTG is occasionally supplied as a mixture with the activator peptide glutathione. Currently, glutathione is industrially produced using a budding yeast, S...
Joseph, Fraulin Lee, So Jung Bryant, Eric Edward Rothstein, Rodney
Published in
Methods in molecular biology (Clifton, N.J.)
The precise organization of the genome inside the cell nucleus is vital to many cell functions including gene expression, cell division, and DNA repair. Here we describe a method to measure pairing of DNA loci during homologous recombination (HR) at a site-specific double-strand break (DSB) in Saccharomyces cerevisiae. This method utilizes a chromo...
Gamble, Dionna Hum, Yee Fang Jinks-Robertson, Sue
Published in
Methods in molecular biology (Clifton, N.J.)
Mitotic double-strand breaks (DSBs) are repaired by recombination with a homologous donor duplex. This process involves the exchange of single DNA strands between the broken molecule and the repair template, giving rise to regions of heteroduplex DNA (hetDNA). The creation of a defined DSB coupled with the use of a sequence-diverged repair template...
Simon, Marie-Noelle Churikov, Dmitri Géli, Vincent
Published in
Methods in molecular biology (Clifton, N.J.)
Upon telomerase inactivation telomeres are getting shorter at each round of DNA replication and progressively lose capping functions and hence protection against homologous recombination. In addition, telomerase-minus cells undergo a round of stochastic DNA damage before the bulk of telomeres become critically short because telomeres are difficult ...
Carlström, Andreas Rzepka, Magdalena Ott, Martin
Published in
Methods in molecular biology (Clifton, N.J.)
The mitochondrial genome encodes only a handful of proteins, but methods to track their synthesis are highly limited. Saccharomyces cerevisiae is a model organism that offers possibilities to expand the classical systems to analyze mitochondrial translation. In this chapter, we present two approaches of monitoring mitochondrial protein synthesis. L...
Bossaert, Sofie Winne, Valérie Van Opstaele, Filip Buyse, Jasper Verreth, Christel Herrera-Malaver, Beatriz Van Geel, Maarten Verstrepen, Kevin J Crauwels, Sam De Rouck, Gert
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Published in
International journal of food microbiology
Currently, there is a strong interest in barrel ageing of finished, conventionally fermented beers, as a novel way to produce sour beers with a rich and complex flavour profile. The production process, however, remains largely a process of trial and error, often resulting in profit losses and inconsistency in quality. To improve product quality and...
Wei, Yan Sun, Ruize Su, Hui Xu, Hao Zhang, Lichuang Huang, Di Liang, Ziwei Hu, Yinchun Zhao, Liqin Lian, Xiaojie
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Published in
Colloids and surfaces. B, Biointerfaces
Using yeast as organic template and PDDA/PSS with opposite charge as polyelectrolyte, CaCO3 was deposited on yeast cells by Layer-by-Layer self-assembly method, and then porous calcium carbonate hybrid microspheres (CaCO3-HMPs) were prepared by calcination. The CaCO3-HMPs were characterized by FT-IR, XRD, SEM and TG. It was found that the prepared ...
Chen, Lu-Zhou Huang, Si-Ling Hou, Jin Guo, Xue-Ping Wang, Feng-Shan Sheng, Ju-Zheng
Published in
Biotechnology for Biofuels
d-Glucaric acid (GA) is a value-added chemical produced from biomass, and has potential applications as a versatile platform chemical, food additive, metal sequestering agent, and therapeutic agent. Marketed GA is currently produced chemically, but increasing demand is driving the search for eco-friendlier and more efficient production approaches. ...