Published in 3 Biotech
Pseudozyma sp. are yeasts that are commercially important due to their production of glycolipid biosurfactants, squalene, itaconic acid, and exopolysaccharide. The search for other analgesia inducing drugs, such as opiates and non-steroidal anti-inflammatory drugs (NSAIDs), as alternatives is beneficial. In this study, the antinociceptive and anti-...
Published in 3 Biotech
Newcastle disease virus is a member of family Paramyxoviridae that infects chicken. Its genome comprises ~15.2 kb negative-sense RNA that encodes six major proteins. The virus encodes various proteins; among all, nucleocapsid (NP) and matrix (M) help in virus replication and its budding from the host cells, respectively. In this study, we investiga...
Published in AMB Express
Transglutaminase (TG) catalyzes the formation of cross-links between proteins. TG from Streptoverticillium mobaraense (SmTG) is used widely in food, cosmetic, biomaterial and medical industries. SmTG is occasionally supplied as a mixture with the activator peptide glutathione. Currently, glutathione is industrially produced using a budding yeast, S...
Published in Methods in molecular biology (Clifton, N.J.)
The precise organization of the genome inside the cell nucleus is vital to many cell functions including gene expression, cell division, and DNA repair. Here we describe a method to measure pairing of DNA loci during homologous recombination (HR) at a site-specific double-strand break (DSB) in Saccharomyces cerevisiae. This method utilizes a chromo...
Published in Methods in molecular biology (Clifton, N.J.)
Mitotic double-strand breaks (DSBs) are repaired by recombination with a homologous donor duplex. This process involves the exchange of single DNA strands between the broken molecule and the repair template, giving rise to regions of heteroduplex DNA (hetDNA). The creation of a defined DSB coupled with the use of a sequence-diverged repair template...
Published in Methods in molecular biology (Clifton, N.J.)
Upon telomerase inactivation telomeres are getting shorter at each round of DNA replication and progressively lose capping functions and hence protection against homologous recombination. In addition, telomerase-minus cells undergo a round of stochastic DNA damage before the bulk of telomeres become critically short because telomeres are difficult ...
Published in Methods in molecular biology (Clifton, N.J.)
The mitochondrial genome encodes only a handful of proteins, but methods to track their synthesis are highly limited. Saccharomyces cerevisiae is a model organism that offers possibilities to expand the classical systems to analyze mitochondrial translation. In this chapter, we present two approaches of monitoring mitochondrial protein synthesis. L...
Published in International journal of food microbiology
Currently, there is a strong interest in barrel ageing of finished, conventionally fermented beers, as a novel way to produce sour beers with a rich and complex flavour profile. The production process, however, remains largely a process of trial and error, often resulting in profit losses and inconsistency in quality. To improve product quality and...
Published in Colloids and surfaces. B, Biointerfaces
Using yeast as organic template and PDDA/PSS with opposite charge as polyelectrolyte, CaCO3 was deposited on yeast cells by Layer-by-Layer self-assembly method, and then porous calcium carbonate hybrid microspheres (CaCO3-HMPs) were prepared by calcination. The CaCO3-HMPs were characterized by FT-IR, XRD, SEM and TG. It was found that the prepared ...
Published in Biotechnology for Biofuels
d-Glucaric acid (GA) is a value-added chemical produced from biomass, and has potential applications as a versatile platform chemical, food additive, metal sequestering agent, and therapeutic agent. Marketed GA is currently produced chemically, but increasing demand is driving the search for eco-friendlier and more efficient production approaches. ...
