Rovini, Amandine Gurnev, Philip A. Beilina, Alexandra Queralt-Martín, María Rosencrans, William Cookson, Mark R. Bezrukov, Sergey M. Rostovtseva, Tatiana K.
Published in
Cellular and Molecular Life Sciences
An intrinsically disordered neuronal protein α-synuclein (αSyn) is known to cause mitochondrial dysfunction, contributing to loss of dopaminergic neurons in Parkinson’s disease. Through yet poorly defined mechanisms, αSyn crosses mitochondrial outer membrane and targets respiratory complexes leading to bioenergetics defects. Here, using neuronally ...
Azhar, Nazila Su, Nana Shabala, Lana Shabala, Sergey
Published in
Plant & cell physiology
This study has investigated mechanisms conferring beneficial effects of exogenous application of 24-epibrassinolides (EBL) on plant growth and performance under saline conditions. Barley seedlings treated with 0.25 mg l-1 EBL showed significant improvements in root hair length, shoot length, shoot fresh weight and relative water content when grown ...
Bryant, Sheenah Shrestha, Nisha Carnig, Paul Kosydar, Samuel Belzeski, Philip Hanna, Charles Fologea, Daniel
Published in
Purinergic Signalling
Lysenin, a pore-forming protein extracted from the coelomic fluid of the earthworm Eisenia foetida, manifests cytolytic activity by inserting large conductance pores in host membranes containing sphingomyelin. In the present study, we found that adenosine phosphates control the biological activity of lysenin channels inserted into planar lipid memb...
Shuvo, Sabbir R Ferens, Fraser G Court, Deborah A
Published in
Biochimica et biophysica acta
A novel feature of the voltage-dependent anion channel (VDAC, mitochondrial porin), is the barrel, comprising an odd number of β-strands and closed by parallel strands. Recent research has focused on the N-terminal segment, which in the available structures, resides in the lumen and is not part of the barrel. In this review, the structural data obt...
Rappaport, Shay M. Teijido, Oscar Hoogerheide, David P. Rostovtseva, Tatiana K. Berezhkovskii, Alexander M. Bezrukov, Sergey M.
Published in
European Biophysics Journal
Hysteresis in the conductance of voltage-sensitive ion channels is observed when the transmembrane voltage is periodically varied with time. Although this phenomenon has been used in studies of gating of the voltage-dependent anion channel, VDAC, from the outer mitochondrial membrane for nearly four decades, full hysteresis curves have never been r...
Freites, J. Alfredo Tobias, Douglas J.
Published in
The Journal of Membrane Biology
Voltage-sensing domains (VSDs) are integral membrane protein units that sense changes in membrane electric potential, and through the resulting conformational changes, regulate a specific function. VSDs confer voltage-sensitivity to a large superfamily of membrane proteins that includes voltage-gated Na+\documentclass[12pt]{minimal} \usepackage{ams...
Jayakannan, Maheswari Bose, Jayakumar Babourina, Olga Rengel, Zed Shabala, Sergey
Published in
Plant Growth Regulation
Soil salinity is one of the major environmental stresses affecting crop production worldwide, costing over $27Bln per year in lost opportunities to agricultural sector and making improved salinity tolerance of crops a critical step for sustainable food production. Salicylic acid (SA) is a signalling molecule known to participate in defence response...
Füll, Yvonne Seebohm, Guiscard Lerche, Holger Maljevic, Snezana
Published in
Pflügers Archiv - European Journal of Physiology
The voltage-gated potassium channels KV7.2 and KV7.3 (KCNQ2/3 genes) play an important role in regulating neuronal excitability. More than 50 KCNQ2/3 mutations have been identified to cause an inherited form of epilepsy in newborns. For two of those (E119G and S122L) found in the S1–S2 region of KV7.2, we previously showed a decreased channel avail...
Bukauskas, Feliksas F.
Published in
The Journal of Membrane Biology
We examined the permeability of connexin36 (Cx36) homotypic gap junction (GJ) channels, expressed in neurons and β-cells of the pancreas, to dyes differing in molecular mass and net charge. Experiments were performed in HeLa cells stably expressing Cx36 tagged with EGFP by combining a dual whole-cell voltage clamp and fluorescence imaging. To asses...
Karmažínová, Mária Baumgart, Joel P. Perez-Reyes, Edward Lacinová, Ľubica
Published in
Pflügers Archiv - European Journal of Physiology
Low-voltage-activated CaV3 Ca2+ channels have an activation threshold around −60 mV, which is lower than the activation threshold of other voltage-dependent calcium channels (VDCCs). The kinetics of their activation at membrane voltages just above the activation threshold is much slower than the activation kinetics of other VDCCs. It was demonstrat...
