Volland, Hervé Girlich, Delphine Laguide, Marine Gonzalez, Camille Paris, Virginie Laroche, Maxime Oueslati, Saoussen Dortet, Laurent Simon, Stéphanie Naas, Thierry
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Published in
Antimicrobial Agents and Chemotherapy
Here, we evaluated the immunochromatographic assay NG-Test Carba 5v2 (NG-Biotech), with improved IMP variant detection on 31 IMP producers, representing the different branches of the IMP phylogeny, including 32 OXA-48, 19 KPC, 12 VIM, 14 NDM, and 13 multiple carbapenemase producers (CPs), 13 CPs that were not targeted, and 13 carbapenemase-negative...
Song, Wonkeun Park, Min-Jeong Jeong, Seri Shin, Dong Hoon Kim, Jae-Seok Kim, Hyun Soo Kim, Han-Sung Lee, Nuri Hong, Jun Sung Jeong, Seok Hoon
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Published in
Annals of Laboratory Medicine
There is an urgent need for accurate and rapid diagnostic assays capable of identifying carbapenemase-producing Enterobacteriaceae (CPE). We assessed the performance of the RESIST-4 O.K.N.V. (OKNV) assay (Coris BioConcept, Gembloux, Belgium) for the identification of oxacillinase (OXA)-48-like-, Klebsiella pneumoniae carbapenemase (KPC)-, New Delhi...
Iovene, Maria Rosaria Pota, Vincenzo Galdiero, Massimiliano Corvino, Giusy Di Lella, Federica Maria Stelitano, Debora Passavanti, Maria Beatrice Pace, Maria Caterina Alfieri, Aniello Di Franco, Sveva
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Published in
World journal of clinical cases
Carbapenem-resistant Enterobacteriaceae has become a significant public health concern as hospital outbreaks are now being frequently reported and these organisms are becoming difficult to treat with the available antibiotics. An outbreak of VIM-producing Serratia marcescens occurred over a period of 11 wk (August, 1 to October, 18) in patients adm...
Baeza, L Lucena Pfennigwerth, N Greissl, C Göttig, S Saleh, A Stelzer, Y Gatermann, S G Hamprecht, A
Published in
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
The aim of this study was to evaluate the performance of five different carbapenemase tests and to develop an algorithm which will permit the detection of most common and rare carbapenemases in routine microbiology laboratories. The immunochromatographic tests CARBA-5 (NG), RESIST-4 O.K.N.V. (Coris), the colorimetric β-CARBA (BioRad), a newly devel...
Lahiri, Surobhi Venkataraman, Rasika Jagan, Akshaya Deshmukh, Gargi Patra, Sudipta Reddy, Vani Sangeetha, V Solanki, Rachana Gupta, Jyoti Patel, Karuna
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Published in
Journal of medical microbiology
Purpose. Rapid and accurate detection of carbapenem resistance is a critical requirement for the selection of appropriate therapy and initiation of infection control measures. Although several tests are available, their use is limited by one or more factors. Phenotypic tests are lengthy, have variable sensitivity and specificity and do not generall...
Li, Jin Hu, Weiwei Li, Min Deng, Shaoli Huang, Qing Lu, Weiping
Published in
Infection and Drug Resistance
Background Metallo-β-lactamase-producing Pseudomonas aeruginosa (MBL-PA) are important causative agents of nosocomial infections and are associated with significant mortality rates, especially in intensive care units. The timely detection and typing of these strains is essential for surveillance, outbreak prevention and antibiotic therapy optimizat...
Dong, Ning Sun, Qiaoling Huang, Yonglu Shu, Lingbin Ye, Lianwei Zhang, Rong Chen, Sheng
Published in
Antimicrobial agents and chemotherapy
We report the identification of a carbapenem-resistant, hypervirulent Klebsiella pneumoniae (hvKp) strain which produced the carbapenemase VIM-1. Genomic analysis showed that the strain belonged to sequence type ST23 and serotype K1, a major hvKp clone, and harbored three resistance-encoding plasmids. Among them, a bla VIM-1-bearing plasmid was fou...
Robador, Alberto Amend, Jan P Finkel, Steven E
Published in
Applied and environmental microbiology
Bacterial populations in long-term stationary-phase (LTSP) laboratory cultures can provide insights into physiological and genetic adaptations to low-energy conditions and population dynamics in natural environments. While overall population density remains stable, these communities are very dynamic and are characterized by the rapid emergence and ...
Bordin, Amanda Trembizki, Ella Windsor, Madeline Wee, Rachel Tan, Lit Yeen Buckley, Cameron Syrmis, Melanie Bergh, Haakon Cottrell, Kyra Zowawi, Hosam M.
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Published in
BMC Infectious Diseases
BackgroundCarbapenemase-producing organisms (CPOs) have emerged as antibiotic-resistant bacteria of global concern. Here we assessed the performance of the Carba (beta) assay, a multiplex real-time PCR assay developed by SpeeDx for the detection of key carbapenemase-encoding genes: KPC, NDM, OXA-48-like, IMP-4-like, and VIM.MethodsDNA extracts of 1...
MacDonald, James Wesley Chibabhai, Vindana
Published in
Access Microbiology
Purpose This study aimed to evaluate the performance of the RESIST-4 O.K.N.V immunochromatographic lateral flow assay for the detection of OXA-48, KPC, NDM and VIM carbapenemases in 100 clinical Enterobacteriaceae isolates using solid culture media. Methodology In total, 100 clinical Enterobacteriaceae isolates with characterized β -lactamase enzym...
