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Effect of thrombin injection on cerebral vascular in rats with subarachnoid hemorrhage.

Published in The Journal of international medical research

SNAC-tag for sequence-specific chemical protein cleavage.

Site-specific protein cleavage is essential for many protein-production protocols and typically requires proteases. We report the development of a chemical protein-cleavage method that is achieved through the use of a sequence-specific nickel-assisted cleavage (SNAC)-tag. We demonstrate that the SNAC-tag can be inserted before both water-soluble an...

Thrombin stimulates gene expression and secretion of IL-11 via protease-activated receptor-1 and regulates extravillous ...

Published in Journal of reproductive immunology

Extravillous trophoblast (EVT) migration and invasion is the crucial step for normal placental development. IL-11 is a cytokine regulating cell migration and invasion in cells and is a critical factor for successful implantation of an embryo. Higher expression of thrombin receptor PAR-1 was reported in early pregnancy. The precise role of thrombin ...

Thrombin promotes pericyte coverage by Tie2 activation in a rat model of intracerebral hemorrhage.

Published in Brain research

Pericyte coverage on the endothelial tubes leads to the formation of a mature and stable microvessel system, which is critical for brain repair after intracerebral hemorrhage (ICH). We report herein that thrombin promotes pericyte coverage by activating Tie2 and the downstream signaling pathway PI3K/Akt in a rat model of ICH. ICH was induced by inj...

Probing light chain mutation effects on thrombin via molecular dynamics simulations and machine learning.

Published in Journal of biomolecular structure & dynamics

Thrombin is a key component for chemotherapeutic and antithrombotic therapy development. As the physiologic and pathologic roles of the light chain still remain vague, here, we continue previous efforts to understand the impacts of the disease-associated single deletion of LYS9 in the light chain. By combining supervised and unsupervised machine le...

Analysis of the role of thrombomodulin in all-trans retinoic acid treatment of coagulation disorders in cancer patients....

BACKGROUND:Clinical studies have shown that all-trans retinoic acid (RA), which is often used in treatment of cancer patients, improves hemostatic parameters and bleeding complications such as disseminated intravascular coagulation (DIC). However, the mechanisms underlying this improvement have yet to be elucidated. In vitro studies have reported t...

Thrombin generation in patients with severe thermal injury.

Published in Burns : journal of the International Society for Burn Injuries

Severe burns can induce a hypercoagulable state which is not depicted in conventional coagulation assays. The thrombin generation assay allows global assessment of coagulation and can identify hypercoagulability. We report changes in thrombin generation in patients after severe burn injury. We measured TGA, rotational thrombelastometry and conventi...