Murafuji, Hidenobu Sugawara, Hajime Goto, Megumi Oyama, Yoshiaki Sakai, Hiroki Imajo, Seiichi Tomoo, Toshiyuki Muto, Tsuyoshi
Published in
Bioorganic & medicinal chemistry
A series of 1,3,6-trisubstituted 1,4-diazepan-7-ones were prepared as kallikrein 7 (KLK7, stratum corneum chymotryptic enzyme) inhibitors. Previously reported compounds 1-3 were potent human KLK7 inhibitors; however, they did not exhibit inhibitory activity against mouse KLK7. Comparison of the human and mouse KLK7 structures reveals the cause of t...
Zhao, Lei Yamaguchi, Yasuto Ge, Xiaomei Robinson, William H. Morser, John Leung, Lawrence L. K.
Published in
Arthritis Research & Therapy
BackgroundChemerin is a chemoattractant involved in immunity that also functions as an adipokine. Chemerin is secreted as an inactive precursor (chem163S), and its activation requires proteolytic cleavages at its C-terminus, involving proteases in coagulation, fibrinolysis, and inflammation. Previously, we found chem158K was the dominant chemerin f...
Hsieh, Huei-Mei Ju, Yu-Ming
Published in
Applied Microbiology and Biotechnology
Termitomyces is a genus of edible mushrooms commonly consumed in Africa and Asia among the mushrooms collected from the wild. Termitomyces mushrooms grow as symbionts in the termite nests, where they produce various enzymes to help termites digest lignocellulosic substrates. Many species of Termitomyces are used by different ethnic groups with ethn...
Zhao, Hong-Yan Feng, Hong
Published in
BMC Biotechnology
BackgroundMesophilic alkaline serine proteases from various bacteria have been commercially applied in a range of industries owing to their high catalytic efficiency and wide substrate specificity. However, these proteases have an optimal catalytic temperature of approximately 50 °C, and their activity decreases significantly at low temperature. Th...
Osei-Adjei, George Huang, Xinxiang Zhang, Yiquan
Published in
World Journal of Microbiology and Biotechnology
Vibrio parahaemolyticus, a Gram-negative bacterium, inhabits marine and estuarine environments and it is a major pathogen responsible globally for most cases of seafood-associated gastroenteritis in humans and acute hepatopancreatic necrosis syndrome in shrimps. There has been a dramatic worldwide increase in V. parahaemolyticus infections over the...
Dvorak, Jan Horn, Martin
Published in
International journal for parasitology
Trematodes, also known as flukes, are phylogenetically ancient parasitic organisms. Due to their importance as human and veterinary parasites, their proteins have been investigated extensively as drug and vaccine targets. Among those, proteases, as crucial enzymes for parasite survival, are considered candidate molecules for anti-parasitic interven...
Anu, Prasannan V. Madanan, Madathiparambil G. Nair, Ananthakrishnan J. Nair, Gangaprasad A. Nair, Govinda Pillai M. Sudhakaran, Perumana R. Satheeshkumar, Padikara K.
Published in
Molecular Biotechnology
Oligopeptidases are enzymes involved in the degradation of short peptides (generally less than 30 amino acids in size) which help pathogens evade the host defence mechanisms. Leptospira is a zoonotic pathogen and causes leptospirosis in mammals. Proteome analysis of Leptospira revealed the presence of oligopeptidase A (OpdA) among other membrane pr...
Wilson, Karl A Tan-Wilson, Anna
Published in
Journal of plant physiology
Legume species differ in whether or not the 7S globulins stored in seeds undergo proteolytic processing during seed development, while preserving the bicupin structure and trimeric assembly necessary for accumulation and packing into protein storage vacuoles. Two such cleavage sites have been documented for the vicilins in pea cotyledons: one in th...
Bao, Jialing Pan, Guoqing Poncz, Mortimer Wei, Junhong Ran, Maoshuang Zhou, Zeyang
Published in
PeerJ
Serpins are a broadly distributed superfamily of protease inhibitors that are present in all kingdoms of life. The acronym, serpin, is derived from their function as potent serine proteases inhibitors. Early studies of serpins focused on their functions in haemostasis since modulating serine proteases activities are essential for coagulation. Addit...
Chen, Hao Ambadapadi, Sriram Dai, Erbin Liu, Liying Yaron, Jordan R Zhang, Liqiang Lucas, Alexandra
Published in
Methods in molecular biology (Clifton, N.J.)
Serpins have a wide range of functions in regulation of serine proteases in the thrombotic cascade and in immune responses, representing up to 2-10% of circulating proteins in the blood. Selected serpins also have cross-class inhibitory actions for cysteine proteases in inflammasome and apoptosis pathways. The arterial and venous systems transport ...
