Martinez, Juan Pinheiro De Oliveira Cai, Guiqin Nachtschatt, Matthias Navone, Laura Zhang, Zhanying Robins, Karen Speight, Robert
Keratins are important structural proteins produced by mammals, birds and reptiles. Keratins usually act as a protective barrier or a mechanical support. Millions of tonnes of keratin wastes and low value co-products are generated every year in the poultry, meat processing, leather and wool industries. Keratinases are proteases able to breakdown ke...
Svarcbahs, Reinis Jäntti, Maria Kilpeläinen, Tommi Julku, Ulrika H Urvas, Lauri Kivioja, Saara Norrbacka, Susanna Myöhänen, Timo T
Published in
Pharmacological research
Prolyl oligopeptidase (PREP) is a serine protease that has been studied particularly in the context of neurodegenerative diseases for decades but its physiological function has remained unclear. We have previously found that PREP negatively regulates beclin1-mediated macroautophagy (autophagy), and that PREP inhibition by a small-molecule inhibitor...
Motta, Flávia Nader Azevedo, Clênia Dos Santos Neves, Beatriz Pereira Araújo, Carla Nunes de Grellier, Philippe Santana, Jaime Martins de Bastos, Izabela Marques Dourado
Published in
Biochimie
Oligopeptidases B (OPB) belong to the S9 prolyl oligopeptidase family and are expressed in prokaryotes, some eukaryotes and in some higher plants. OPB is not found in any of the mammalian genomes available to date. Evidences indicate that OPB participates in the infections caused by trypanosomatids Trypanosoma cruzi, Leishmania spp. and Trypanosoma...
Ahmad, Malik Shoaib Akbar, Zeeshan Choudhary, M Iqbal
Published in
Bioorganic chemistry
Proteinase K is a stable serine protease, crystallized and extensively used in the study of molecular interactions at the atomic level. During the current study, crystal structure of proteinase K with thiourea (TU) was solved at 1.45 Å (angstrom) resolution. Proteinase K showed its binding affinity with thiourea after soaking with 200 mM (millimola...
Rut, Wioletta Nielsen, Nis Valentin Czarna, Justyna Poreba, Marcin Kanse, Sandip M Drag, Marcin
Published in
Thrombosis research
The zymogen form of circulating Factor VII activating protease (FSAP) is activated by histones that are released as a consequence of tissue damage or excessive inflammation. This is likely to have consequences in a number of disease conditions such as stroke, atherosclerosis, liver fibrosis, thrombosis and cancer. To investigate the existence, as w...
Billinger, Erika Zuo, Shusheng Lundmark, Kristoffer Johansson, Gunnar
Published in
Analytical biochemistry
The interaction between pancreatic proteases and a serine protease inhibitor purified from potato tubers was investigated by chromatography-coupled light scattering measurements. The molar mass distribution in the chromatogram was compared to theoretical values calculated for the different possible combinations of complexes and free components by t...
Kuniyoshi, Alexandre Kazuo Kodama, Roberto Tadashi Cajado-Carvalho, Daniela Iwai, Leo Kei Kitano, Eduardo da Silva, Cristiane Castilho Fernandes Duzzi, Bruno Dias da Silva, Wilmar Portaro, Fernanda Calheta
Published in
Toxicon : official journal of the International Society on Toxinology
In Brazil, snakes from the Bothrops genus are responsible for thousands of accidents, and their venoms are mainly made up of proteolytic enzymes. Although the antibothropic serum produced by the Butantan Institute is remarkable in saving lives, studies show that some symptoms observed in cases of envenoming are not efficiently neutralized. Moreover...
Matkawala, Fatema Nighojkar, Sadhana Kumar, Anil Nighojkar, Anand
Published in
Heliyon
Alkaline proteases have several industrial applications. In the present study, newly isolated Neocosmospora sp. N1 was screened as hyper producer of serine protease . A multimeric protease of the fungus was purified to homogeneity till 96.78 fold purification with 22.51% recovery. The homogeneity of purified enzyme was checked by native PAGE and it...
Kumar, Bhumika Verma, Sonia Kashif, Mohammad Sharma, Ruby Atul, Dixit, Rajnikant Singh, Agam P Pande, Veena Saxena, Ajay K Abid, Mohammad
...
Published in
International journal of biological macromolecules
Metacaspases are clan CD cysteine peptidases found in plants, fungi and protozoa that possess a conserved Peptidase_C14 domain, homologous to the human caspases and a catalytic His/Cys dyad. Earlier reports have indicated the role of metacaspases in cell death; however, metacaspases of human malaria parasite remains poorly understood. In this study...
Menaldo, Danilo L Costa, Tássia R Ribeiro, Diego L Zambuzi, Fabiana A Antunes, Lusânia M G Castro, Fabíola A Frantz, Fabiani G Sampaio, Suely V
Published in
Toxicology in vitro : an international journal published in association with BIBRA
The aim of this study was to evaluate the immunomodulatory effects of two toxins from Bothrops snake venoms (the P-I metalloprotease Batroxase and the thrombin-like serine protease Moojase) on human peripheral blood mononuclear cells (PBMC), also investigating changes in the expression of genes related to epigenetic alterations and their immunother...