An essential mechanism for severe acute respiratory syndrome coronavirus 1 (SARS-CoV-1) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection begins with the viral spike protein binding to the human receptor protein angiotensin-converting enzyme II (ACE2). Here, we describe a stepwise engineering approach to generate a set of a...
Published in Science China. Life sciences
Phloretin is an important skin-lightening and depigmenting agent from the peel of apples. Although de novo production of phloretin has been realized in microbes using the natural pathway from plants, the efficiency of phloretin production is still not enough for industrial application. Here, we established an artificial pathway in the yeast to prod...
Published in Biotechnology for Biofuels
BackgroundThe use of thermotolerant yeast strains can improve the efficiency of ethanol fermentation, allowing fermentation to occur at temperatures higher than 40 °C. This characteristic could benefit traditional bio-ethanol production and allow simultaneous saccharification and fermentation (SSF) of starch or lignocellulosic biomass.ResultsWe ide...
Published in mBio
Whereas the yeast Saccharomyces cerevisiae shows great preference for glucose as a carbon source, a deletion mutant in trehalose-6-phosphate synthase, tps1Δ, is highly sensitive to even a few millimolar glucose, which triggers apoptosis and cell death. Glucose addition to tps1Δ cells causes deregulation of glycolysis with hyperaccumulation of metab...
Published in Heliyon
Food science, Microbiology, Natural product chemistry, Cyanidin, Saccharomyces cerevisiae , Yap1, Skn7, Visible light
Published in Sheng wu gong cheng xue bao = Chinese journal of biotechnology
内切葡聚糖酶 (EG) 是纤维素酶的重要组分，在纤维素降解酶系中发辉重要作用。由于天然微生物来源的内切葡聚糖酶产量低，极大地制约了其生产和应用，所以对内切葡聚糖酶进行高效异源表达是解决这一问题的有效途径。为了获得高效内切葡聚糖酶酿酒酵母工程菌，本研究从纤维梭菌中克隆了内切葡聚糖酶 (EG) 基因，全长1 996 bp，编码440 个氨基酸，并与来源于酿酒酵母的PGK 启动子序列、来源于pPIC9K 质粒的α-信号肽序列以及来源于pSH65 质粒的CYC1 终止子序列通过重叠延伸PCR 法构建完整表达盒 (PαEGC)，通过整合rDNA 的方法构建内切葡聚糖酶酿酒酵母的表达载体，在酿酒酵母中进行内切葡聚糖酶的随机多拷贝表达。利用微滴数字PCR 鉴定内切葡聚糖酶拷贝数，并探索拷贝数与蛋白表达量之...
Published in Toxins
Microcystins (MCs) are hepatotoxins produced by some cyanobacteria. They are cyclic peptides that inhibit the serine/threonine protein phosphatases (PPs) PP1 and PP2A, especially PP2A. The inhibition of PP2A triggers a series of molecular events, which are responsible for most MC cytotoxic and genotoxic effects on animal cells. It is also known tha...
Published in Microbial Cell Factories
BackgroundIn Saccharomyces cerevisiae, alpha-glucosidase (maltase) is a key enzyme in maltose metabolism. In addition, the overexpression of the alpha-glucosidase-encoding gene MAL62 has been shown to increase the freezing tolerance of yeast in lean dough. However, its cryoprotection mechanism is still not clear.ResultsRNA sequencing (RNA-seq) reve...
Published in ACS synthetic biology
Genome engineering of microorganisms has become a standard in microbial biotechnologies. Several efficient tools are available for the genetic manipulation of model bacteria such as Escherichia coli and Bacillus subtilis, or the yeast Saccharomyces cerevisiae. Difficulties arise when transferring these tools to nonmodel organisms. Synthetic biology...
Published in Antioxidants
Reactive sulfane sulfur, including persulfide and polysulfide, is a type of regular cellular component, playing an antioxidant role. Its function may be organelle-dependent; however, the shortage of probes for detecting organellar reactive sulfane sulfur has hindered further investigation. Herein, we reported a red fluorescent protein (mCherry)-bas...
