Journal of periodontal & implant science
We demonstrated that CD103(+) DCs can promote antigen-specific Tregs from naive T cells, when used as APCs for an epitope peptide from P. gingivalis HSP60. RA was an effective reagent that induces mature DCs with the typical phenotypic expression of CD103 that demonstrated the functional capability to promote antigen-specific Tregs.
National Science Foundation (NSF) through the MRSEC Program (DMR-1121053); MRSEC Program of the NSF (DMR-1121053); NIH Innovator Award; US National Institutes of Health (R01 DE018468); Human Frontiers of Science Program
Eight brain-derived proteins were evaluated regarding their potential for further development as a blood-based biomarker for malignant gliomas. Plasma levels for glial fibrillary acidic protein, neurogranin, brain-derived neurotrophic factor, intracellular adhesion molecule 5, metallothionein-3, beta-synuclein, S100 and neuron specific enolase were...
Analytica chimica acta
A C18 pipette-tip electrospray ionization mass spectrometry technique was developed for rapid analysis of raw solution samples. In this technique, a C18 pipette tip was employed for rapid purification and enrichment of analytes in raw sample solutions. The adsorbed analytes were eluted by solvents supplied by a syringe and a syringe pump, and a hig...
Journal of Polymers and the Environment
Proteins can be made into polymer materials through plasticization. Here, wheat gluten is plasticized with its own tryptic hydrolysate. Wheat gluten hydrolysate largely consists of small molecular weight peptides as measured by size exclusion chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Elastic modulus decreases 3.8...
UNLABELLED:In macromolecular crystallography, the agreement between observed and predicted structure factors (Rcryst and Rfree ) is seldom better than 20%. This is much larger than the estimate of experimental error (Rmerge ). The difference between Rcryst and Rmerge is the R-factor gap. There is no such gap in small-molecule crystallography, for w...
BMC research notes
We advocate the use of the Gong and Rose transition midpoint method for computing solvent accessible surface area due to its computational ease, physical basis, and performance in terms of relationships with thermodynamic parameters.
Annals of clinical biochemistry
Multiple myeloma (MM) is a plasma cell dyscrasia characterized by the malignant proliferation of a plasma cell clone that produces a monoclonal immunoglobulin. Diagnosis and management of patients with monoclonal gammopathies depend on accurate identification and characterization of monoclonal proteins. We present a 67-year-old male patient with an...
Cross-linking combined with MS involves enzymatic digestion of cross-linked proteins and identifying cross-linked peptides. Assignment of cross-linked peptide masses requires a search of all possible binary combinations of peptides from the cross-linked proteins' sequences, which becomes impractical with increasing complexity of the protein system ...