Published in Solid state nuclear magnetic resonance
The successful synthesis and solid state NMR characterization of silica-based organic-inorganic hybrid materials is presented. For this, collagen-like peptides are immobilized on carboxylate functionalized mesoporous silica (COOH/SiOx) materials. A pre-activation of the silica material with TSTU (O-(N-Succinimidyl)-N,N,N',N'-tetramethyluronium tetr...
Published in Oecologia
Changes in the 12C/13C ratio (expressed as δ13C) of soil organic C (SOC) has been observed over long time scales and with depth in soil profiles. The changes are ascribed to the different reaction kinetics of 12C and 13C isotopes and the different isotopic composition of various SOC pool components. However, experimental verification of the subtle ...
Published in Journal of magnetic resonance (San Diego, Calif. : 1997)
2D NMR (1)H-X (X=(15)N or (13)C) HSQC spectra contain cross-peaks for all XHn moieties. Multiplicity-edited(1)H-(13)C HSQC pulse sequences generate opposite signs between peaks of CH(2) and CH/CH(3) at a cost of lower signal-to-noise due to the (13)C T(2) relaxation during an additional 1/(1)JCH period. Such CHn-editing experiments are useful in as...
Published in Environmental Earth Sciences
This study examined nitrogen (N) pollution characteristics based on water quality monitoring of the Ashi River and analyzed N sources by stable isotope tracer (SIT) techniques during a hydrological year in 2011. Twenty-two evenly distributed sites were sampled in the mainstream and tributaries of Ashi River during the temperate, wet, and dry season...
Published in Plant and Soil
AimsThe objectives of this study were to compare techniques for measuring biological nitrogen fixation (BNF) and to assess how fertiliser N input affects the balance between BNF and sustainable herbage production on perennial ryegrass (Lolium perenne L.)/white clover (Trifolium repens L.) grassland.MethodsBiological N fixation and herbage productio...
Published in Biology and Fertility of Soils
The effect of elevated carbon dioxide (CO2) concentration on symbiotic nitrogen fixation in soybean under open-air conditions has not been reported. Two soybean cultivars (Glycine max (L.) Merr. cv. Zhonghuang 13 and cv. Zhonghuang 35) were grown to maturity under ambient (415 ± 16 μmol mol−1) and elevated (550 ± 17 μmol mol−1) [CO2] at the free-ai...
Published in Journal of magnetic resonance (San Diego, Calif. : 1997)
3D H2BC is introduced for heteronuclear assignment on natural abundance samples even for biomolecules up to at least 10 kDa in low millimolar concentrations as an overnight experiment using the latest generation of cryogenically cooled probes. The short pulse sequence duration of H2BC is maintained in the 3D version due to multiple use of the const...
Published in Plant and Soil
Disentangling the autotrophic and heterotrophic components of soil CO2 efflux is critical to understanding the role of soil system in terrestrial carbon (C) cycling. In this study, we combined a stable C-isotope natural abundance approach with the trenched plot method to determine if root exclusion significantly affected the isotopic composition (δ...
3D H2BC is introduced for heteronuclear assignment on natural abundance samples even for biomolecules up to at least 10 kDa in low millimolar concentrations as an overnight experiment using the latest generation of cryogenically cooled probes. The short pulse sequence duration of H2BC is maintained in the 3D version due to multiple use of the const...
3D H2BC is introduced for heteronuclear assignment on natural abundance samples even for biomolecules up to at least 10 kDa in low millimolar concentrations as an overnight experiment using the latest generation of cryogenically cooled probes. The short pulse sequence duration of H2BC is maintained in the 3D version due to multiple use of the const...
