The effect of vitamin K3 (2-methyl-1,4-naphthoquinone) on Adriamycin (ADR) induced growth inhibition of drug sensitive and multidrug resistant P388 leukemia cells was evaluated. Exposure to ADR concentrations of 100-5000 ng simultaneously with 1-mu-M vitamin K3 elicited an enhanced inhibition of tumor cell survival. The effect of treatment with ADR...
To determine the clinical significance of the MDR1 gene and its transcript P-glycoprotein (PGP) expression in lung cancer, ninety-one surgical lung cancer samples and normal lung tissues were analyzed. They consisted of 85 NSCLC (84 untreated and one treated) and 6 SCLC (three untreated and three treated). MDR1 messenger RNA (mRNA) levels in all su...
Published in Parasitology Research
The role of membrane drug-transport mechanisms in resistance to anthelmintics was examined using a flow cytometry method. This method was adapted from assays developed for the study of similar mechanisms in tumor cells. Rhodamine 123, a P-glycoprotein transport probe, associated with the reversal agent verapamil gave a significantly higher level of...
Published in Zeitschrift für Naturforschung C
Rhesus monkey kidney MA104 cells are a polarized epithelium with some unusual characteristics, including a resistance to ouabain, although their Na+-K+-ATPase has normal affinity with this drug. This work suggests that MA104 cells have high expression of functionally P-glycoprotein in their membranes. This was established using four complementary m...
Published in International Orthopaedics
Multidrug resistance (MDR), mediated by P-glycoprotein (P-gp), is an in vitro phenomenon observed within tumour cells, suggesting cross-resistance to unrelated drugs, and expression of P-gp may therefore affect the prognosis and incidence of recurrence after treatment. The mutant p53 protein causes reduced tumour suppression. Co-expression of p53 a...
Published in Parasitology Research
Our previous work has shown that drug efflux pumps close to MDR1 P-glycoprotein (Pgp) can regulate anthelmintic efflux in nematodes in a way similar to that of the mutidrug resistance system (MDR) in vertebrate cancer cells. In the present study, the role of the glycosylation of Pgp was studied using a lectin specific for the α-mannosyl residues (L...
Published in Arthritis Res Ther
Published in Infection
Background:Patients with HIV infection may be a valuable target for assessing the impact of drug-resistant tuberculosis (TB).Patients and Methods:An observational, prospective study was conducted in 96 infectious disease hospital units in Italy during 1999–2000. A total of 140 HIV-infected patients with diagnosis of TB and with an isolate tested fo...
Published in Medicinal Chemistry Research
This paper summarizes the results from molecular modeling of various multidrug resistance (MDR) modulators and the MDR protein P-glycoprotein (P-gp). Highly predictive 3D-QSAR models are obtained that can be used for anti-MDR activity prediction. The pharmacophore patterns of various drugs are identified. Models of P-gp are developed and a hypothes...
Published in Critical Care
