Vereecke, Nick Bokma, Jade Haesebrouck, Freddy Nauwynck, Hans Boyen, Filip Pardon, Bart Theuns, Sebastiaan
Published in
BMC Bioinformatics
BackgroundImplementation of Third-Generation Sequencing approaches for Whole Genome Sequencing (WGS) all-in-one diagnostics in human and veterinary medicine, requires the rapid and accurate generation of consensus genomes. Over the last years, Oxford Nanopore Technologies (ONT) released various new devices (e.g. the Flongle R9.4.1 flow cell) and bi...
Chang, Ming-Ling Yang, Zinger Yang, Sien-Sing
Adipose tissue is a highly dynamic endocrine tissue and constitutes a central node in the interorgan crosstalk network through adipokines, which cause pleiotropic effects, including the modulation of angiogenesis, metabolism, and inflammation. Specifically, digestive cancers grow anatomically near adipose tissue. During their interaction with cance...
Ghanta, Krishna S. Mello, Craig C.
CRISPR genome editing has revolutionized genetics in many organisms. In the nematode Caenorhabditis elegans one injection into each of the two gonad arms of an adult hermaphrodite exposes hundreds of meiotic germ cells to editing mixtures, permitting the recovery of multiple indels or small precision edits from each successfully injected animal. Un...
Ly, Socheata
Mutant mRNA and protein both contribute to the clinical manifestation of many repeat-associated neurodegenerative and neuromuscular disorders. The presence of nuclear RNA clusters is a feature shared amongst these diseases, such as C9ORF72/ALS and myotonic dystrophy 1/2 (DM1/2); however, this pathological hallmark has not been conclusively demonstr...
Parsi, Sepideh Qiao, Tao Ostrow, Lyle Wilfred Rust, Marco B. Xu, Zuoshang
Amyotrophic lateral sclerosis (ALS), also known as Lou Gehrig’s disease is the most common form of motor neuron disease. In familial ALS, Multiple mutations of, PFN1 gene a well-known actin-binding protein have been linked to ALS disease recently. Phosphorylation in many degenerative conditions plays an important role in disease mechanism but its p...
Zhang, Bi Ning Liu, Yu Yang, Qichen Leung, Pui Ying Wang, Chengdong Tham, Clement C. Chan, Sun On Pang, Chi Pui Chen, Li Jia Dekker, Job
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Previously, we identified RAD21(R450C) from a peripheral sclerocornea pedigree. Injection of this rad21 variant mRNA into Xenopus laevis embryos disrupted the organization of corneal stroma fibrils. To understand the mechanisms of RAD21-mediated corneal stroma defects, gene expression and chromosome conformation analysis were performed using cells ...
Choi, So Yoen Lee, Ju-Hyun Chung, Ah-Young Jo, Youhwa Shin, Joo-Ho Park, Hae-Chul Kim, Hyun Lopez-Gonzalez, Rodrigo Ryu, Jae Ryun Sun, Woong
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Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease caused by progressive loss of motor neurons (MNs) and subsequent muscle weakness. These pathological features are associated with numerous cellular changes, including alteration in mitochondrial morphology and function. However, the molecular mechanisms associating mitochondri...
Hu, Xin Xing, Yishen Ren, Ling Wang, Yahui Li, Qian Yang, Qiyuan Du, Min Xu, Lingyang Willems, Luc Li, Junya
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miR-23a, a member of the miR-23a/24-2/27a cluster, has been demonstrated to play pivotal roles in many cellular activities. However, the mechanisms of how bta-miR-23a controls the myogenic differentiation (MD) of PDGFRalpha(-) bovine progenitor cells (bPCs) remain poorly understood. In the present work, bta-miR-23a expression was increased during t...
Liu, Wallace H. Zheng, Jie Feldman, Jessica L. Klein, Mark A. Kuznetsov, Vyacheslav I. Peterson, Craig L. Griffin, Patrick R. Denu, John M.
The protein deacetylase SIRT6 maintains cellular homeostasis through multiple pathways that include the deacetylation of histone H3 and repression of transcription. Prior work suggests that SIRT6 is associated with chromatin and can substantially reduce global levels of H3 acetylation, but how SIRT6 is able to accomplish this feat is unknown. Here,...
Vangala, Pranitha Murphy, Rachel Quinodoz, Sofia A. Gellatly, Kyle J. McDonel, Patrick E. Guttman, Mitchell Garber, Manuel
Eukaryotic gene expression regulation involves thousands of distal regulatory elements. Understanding the quantitative contribution of individual enhancers to gene expression is critical for assessing the role of disease-associated genetic risk variants. Yet, we lack the ability to accurately link genes with their distal regulatory elements. To add...
