We present here an improved RNA purification method using fast performance liquid chromatography (FPLC) size-exclusion chromatography in place of denaturing polyacrylamide gel electrophoresis (PAGE). The method allows preparation of milligram quantities of pure RNA in a single day. As RNA oligonucleotides behave differently from globular proteins i...
Published in Radiation Medicine
PurposeDuring CyberKnife treatment, the 6D correction method is used to correct patient positional errors, including rotational ones. We developed novel correction methods for translating rotational errors into 3D, with the aim of making their correction safer than with 6D correction and as accurate as possible.Materials and methodsThese novel corr...
Published in Biotechnology Letters
A rapid, inexpensive and reliable procedure for separation and purification of C-phycocyanin (C-PC) and allophycocyanin (APC) from Arthronema africanum based on a previously described rivanol-sulfate method for C-PC purification was developed. Exclusion of NaCl from the extraction buffer resulted in complete separation of APC and C-PC, two-fold red...
Efter att den så kallade zebralagen infördes år 2000 har olyckorna vid övergångställen ökat, både påkörning av fotgängare och upphinnandeolyckor. Detta skulle delvis kunna förklaras av att bilisterna alltför sent upptäcker fotgängare som står i begrepp att korsa gatan, vilket kan vara speciellt uttalat i mörkertrafik. För att minska riskerna vid öv...
gb-2007-8-1-r4.fm co m m ent review s repo rts depo sited research refereed research interactio ns info rm atio Open Access2007Chenet al.Volume 8, Issue 1, Article R4Method Clustering of genes into regulons using integrated modeling-COGRIM Guang Chen*†, Shane T Jensen‡ and Christian J Stoeckert Jr†§ Addresses: *Department of Bioengineering, Univers...
This report describes the generation of novel encapsidated RNA particles and their evaluation as in-tube internal controls in diagnostic real-time reverse-transcription PCR (rRT-PCR) assays for the detection of RNA viruses. A cassette containing sequences of 2 diagnostic primer sets for foot-and-mouth disease virus (FMDV) and a set for swine vesicu...
A novel approach for finding a list of features that are commonly perturbed in two or more experiments, quantifying the evidence of dependence between the experiments by a ratio.
This report describes the generation of novel encapsidated RNA particles and their evaluation as in-tube internal controls in diagnostic real-time reverse-transcription PCR (rRT-PCR) assays for the detection of RNA viruses. A cassette containing sequences of 2 diagnostic primer sets for foot-and-mouth disease virus (FMDV) and a set for swine vesicu...
3D BLAST, a novel protein structure database search tool, is a useful tool for analysing novel structures, capable of returning a list of aligned structures ordered according to E-values.
A novel approach for finding a list of features that are commonly perturbed in two or more experiments, quantifying the evidence of dependence between the experiments by a ratio.
