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Isolation of nucleic acids using silicon dioxide powder as a tool for environmental monitoring

Published in Environmental Monitoring and Assessment

Isolation and purification of nucleic acids are basic laboratory procedures used in molecular analysis supporting determination of organisms in environmental monitoring. However, many different methods of isolation are commonly used, often being designed for a particular type of DNA extraction. While researchers commonly decide on commercial isolat...

Ultrasensitive loop mediated isothermal amplification (US-LAMP) to detect malaria for elimination

Published in Malaria Journal

BackgroundMalaria elimination requires diagnostic methods able to detect parasite levels well below what is currently possible with microscopy and rapid diagnostic tests. This is particularly true in surveillance of malaria at the population level that includes so-called “asymptomatic” individuals.MethodsThe development of the first ultrasensitive ...

New method for environmental monitoring in armed conflict zones: a case study of Syria

Published in Environmental Monitoring and Assessment

Today, armed conflict affects some twenty countries, covering an area making up 11% of the surface area of the Earth. Any degradation of nature in these areas represents a harmful depletion of the world’s natural heritage. Despite this, environmental issues are neglected during these periods of conflict, considered secondary to the urgency of resto...

Toward an integration of musicological methods into fan video studies

Methods are emerging regarding the analysis of fan videos and vidding. In an expansion of existing analytical methods, I add musical analysis to the repertoire. Assessing music on a deeper, more conscious level takes into account the affective contributions of music in vids, as well as how elements of music contribute to the structuring and creatio...

Wet Dust Sampler—a Sampling Method for Road Dust Quantification and Analyses

Published in Water, Air, & Soil Pollution

In northern countries, the climate, and consequently the use of studded tyres and winter traction sanding, causes accumulation of road dust over winter and spring, resulting in high PM10 concentrations during springtime dusting events. To quantify the dust at the road surface, a method—the wet dust sampler (WDS)—was developed allowing repeatable sa...

A novel method to establish glucocorticoid resistant acute lymphoblastic leukemia cell lines

Published in Journal of Experimental & Clinical Cancer Research

BackgroundDrug-resistant cell lines, established from drug-sensitive cell lines by drug exposure in vitro, are the most useful cancer models in studies on the mechanism of chemoresistance. However, the success rate of the traditional approaches to construct such cell lines is low because a long time is required for the addition of drugs.MethodsA ce...