Menke, Wilhelm Radunz, Alfons Koenig, Friederike
Published in
Zeitschrift für Naturforschung C
Fragments of the thylakoid membrane, obtained by ultrasonication of stroma-freed chloroplasts and subsequent fractioning centrifugation, aggregate in buffer solutions of suitable ionic strength in the pH-range of 6.0-8.5. This aggregation leads to the formation of vesicles and membranes, which may reach a size of up to 0.5 mm. A suspension of the f...
Yckowski, N Brody, S.S.
Published in
Zeitschrift für Naturforschung C
The surface properties of mixed monomolecular films of retinal and phospholipids were studied at a nitrogen/water interface. The subphase was glycine buffer pH 10.5 with an ionic strength of 0.1. Monomolecular films of retinal in the presence of amino acids were also measured. The area per molecule, at a surface pressure of 10 dyn/cm, A10 , in the ...
Karan, Jeffrey Brody, Seymour Steven
Published in
Zeitschrift für Naturforschung C
The surface pressure was measured as a function of area/molecule for chlorophyll a (Chl) and cytochrome c (Cyt) at a heptane-water interface. At a surface pressure of 6 dyn/cm the area per molecule of Chl(A6) = 113 ± 6 Å2, for reduced Cyt c (red. Cyt) the A6 = 5000 ± 300 Å2 and for oxidized Cyt c (ox. Cyt) A6= 4100 ± 250 Å2. Cyt appears to denature...
Puppala, N. Brody, S. S.
Published in
Zeitschrift für Naturforschung C
The surface properties of mixed monomolecular films of retinal and phospholipids (p. lipids) are measured as a function of mole fraction at a nitrogen-water interface. An acid pH of 6.0 is maintained in the aqueous phase. Before irradiation the surface potential ∆V for 9-cis retinal, 11-cis retinal, phosphatidyl serine (PS) and phosphatidyl ethanol...
Weltzien, Hans Ulrich
Published in
Zeitschrift für Naturforschung C
The effects of lysolecithin and of 2 synthetic ether-desoxy lysolecithin analogs, containing alkyl residues of 16 or 12 carbon atoms, on the agglutination kinetics of calf and rabbit thymocytes by concanavalin A (Con A) were investigated. Unlike the natural lysolecithin, these synthetic analogs are resistant to metabolism by membrane associated enz...
Van Den Driessche, T. Dujardin, E. Magnusson, A. Sironval, Cyrille
peer reviewed / Acetabularia mediterranea algae, grown in 3 different light dark regimes, were frozen in liquid nitrogen at c.t.0 and c.t.6 and a record made of 77°K fluorescence emission spectra of their chloroplasts. Algae grown under LD cycles exhibited a clear circadian rhythm of oxygen production. The low temperature fluorescence emission spec...
Powell, JT Järlfors, U Brew, K
Fat globule membranes have been isolated from bovine colostrum and bovine milk by the dispersion of the fat in sucrose solutions at 4 degrees C and fractionation by centrifugation through discontinuous sucrose gradients. The morphology and enzymic characteristics of the separated fractions were examined. Fractions comprising a large proportion of t...
Bondy, Stephen C Harrington, Marilyn E
The high affinity binding of several amino acids to various membrane and protein preparations has been measured. Binding of radioactive amino acids suspected of being neurotransmitters and also of leucine and tyrosine to brain, liver and heart muscle membranes was saturable, reversible and stereospecific. Similar characteristics were found using ch...
Karan, Hiroko Ito Hirsch, Rhoda Elison Brody, Seymour Steven
Published in
Zeitschrift für Naturforschung C
Stability and regenerability of the absorption spectrum of CTAB solubilized rhodopsin at an air-water interface is studied. The spectral properties of rhodopsin films in the dark are stable more than 150 minutes. When rhodopsin is bleached (40 - 50%) and maintained in highly com pressed films recovery of the spectrum is observed. The recovery is 5...
Rich, Marvin Brody, Seymour Steven
Published in
Zeitschrift für Naturforschung C
Asymmetric and symmetric bilayer membrane were formed from monolayers of egg lecithin and various retinal isomers. Capacitance of the asymmetric all-trans retinal/lecithin bilayer is 1.2 ± 0.1 µF/cm2. Asymmetrically formed membranes of 13-cis retinal/lecithin and 9-cis retinal lecithin were found to be 0.83 ± 0.06 and 0.56 ± 0.05 µF/cm2, respective...
