Hanning, Nikita De Man, Joris G. De Winter, Benedicte Y.
Published in
Bio-protocol
Myeloperoxidase (MPO) is an enzyme contained in lysosomal azurophilic granules of neutrophils. MPO activity has been shown to correlate with the number of neutrophils in histological sections of the gastrointestinal tract and is therefore accepted as a biomarker of neutrophil invasion in the gut. This protocol describes an easy, cost-effective kine...
Viola, Maria Francesca; Chavero-Pieres, Marta; Modave, Elodie; 121776; Delfini, Marcello; 131650; Stakenborg, Nathalie; 85528; Estevez, Maria Cuende; Fabre, Naomi; Appeltans, Iris; Martens, Tobie; 99095; Vandereyken, Katy; 95374;
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Correct development and maturation of the enteric nervous system (ENS) is critical for survival1. At birth, the ENS is immature and requires considerable refinement to exert its functions in adulthood2. Here we demonstrate that resident macrophages of the muscularis externa (MMϕ) refine the ENS early in life by pruning synapses and phagocytosing en...
Shalon, Dari Culver, Rebecca Neal Grembi, Jessica A Folz, Jacob Treit, Peter V Shi, Handuo Rosenberger, Florian A Dethlefsen, Les Meng, Xiandong Yaffe, Eitan
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The spatiotemporal structure of the human microbiome1,2, proteome3 and metabolome4,5 reflects and determines regional intestinal physiology and may have implications for disease6. Yet, little is known about the distribution of microorganisms, their environment and their biochemical activity in the gut because of reliance on stool samples and limite...
Vötterl, Julia Christiane Klinsoda, Jutamat Koger, Simone Hennig-Pauka, Isabel Verhovsek, Doris Metzler-Zebeli, Barbara U
Published in
Animal bioscience
Dietary phytase increases bioavailability of phytate-bound phosphorus (P) in pig nutrition affecting dietary calcium (Ca) to P ratio, intestinal uptake, and systemic utilization of both minerals, which may contribute to improper bone mineralization. We used phytase to assess long-term effects of two dietary available P (aP) levels using a one-phase...
Wang, Yu-Chen Cao, Yang Pan, Calvin Zhou, Zhiqiang Yang, Lili Lusis, Aldons J
The small intestine plays a key role in immunity and mediates inflammatory responses to high fat diets. We have used single-cell RNA-sequencing (scRNA-seq) and statistical modeling to examine gaps in our understanding of the dynamic properties of intestinal cells and underlying cellular mechanisms. Our scRNA-seq and flow cytometry studies of differ...
Ramsubeik, Shayne Uzal, Francisco Stoute, Simone Jerry Michmali, Carmen
Four turkeys from a commercial flock with acutely elevated mortality levels were submitted for postmortem examination and diagnostic workup. No clinical signs had been observed before death. On gross examination, hemorrhage and necrosis were present throughout the intestinal tracts, and the spleens were markedly enlarged and speckled. Microscopical...
Beldowska, Aleksandra Barszcz, Marcin Dunislawska, Aleksandra
Published in
Journal of Animal Science and Biotechnology
The relationship between the intestines and their microbiota, the liver, and the neuronal system is called the gut-liver-brain axis. This relationship has been studied and observed for a relatively short time but is considered in the development of research focused on, e.g., liver diseases and intestinal dysbiosis. The role of the gut microbiota in...
Zeng, Yi Yang, Fan Hu, Xiaoyan Zhu, Fei Chen, Weixia Lin, Wei
Published in
European radiology
Transmural intestinal necrosis (TIN) is related to high mortality in patients with acute mesenteric ischemia (AMI). Radiological predictive factors of TIN in AMI remains controversial. This study aimed to identify the CT-based predictive factors of TIN in AMI. EMBASE and PUBMED were searched for publications predicting TIN using radiological featur...
Wang, Qianqian Wang, Jiafang Tokhtaeva, Elmira Li, Zhen Martín, Martín Ling, Xuefeng Dunn, James
The intestinal muscle layers execute various gut wall movements to achieve controlled propulsion and mixing of intestinal content. Engineering intestinal muscle layers with complex contractile function is critical for developing bioartificial intestinal tissue to treat patients with short bowel syndrome. Here, the first demonstration of a living in...
Childs, Charlie J Holloway, Emily M Sweet, Caden W Tsai, Yu-Hwai Wu, Angeline Vallie, Abigail Eiken, Madeline K Capeling, Meghan M Zwick, Rachel K Palikuqi, Brisa
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Epithelial organoids derived from intestinal tissue, called enteroids, recapitulate many aspects of the organ in vitro and can be used for biological discovery, personalized medicine, and drug development. Here, we interrogated the cell signaling environment within the developing human intestine to identify niche cues that may be important for epit...
