Hu, Zhengzheng Li, Yuchen Du, Heng Ren, Junxiao Zheng, Xianrui Wei, Kejian Liu, Jianfeng
Published in
BMC Genomics
BackgroundPorcine epidemic diarrhea virus (PEDV) is a causative agent of serious viral enteric disease in suckling pigs. Such diseases cause considerable economic losses in the global swine industry. Enhancing our knowledge of PEDV-induced transcriptomic responses in host cells is imperative to understanding the molecular mechanisms involved in the...
Wang, Haiyue Jiang, Yuming Li, Bailiang Cui, Yi Li, Dengwang Li, Ruijiang
Published in
Cancers
Hepatocellular carcinoma (HCC) is a heterogeneous disease with diverse characteristics and outcomes. Here, we aim to develop a histological classification for HCC by integrating computational imaging features of the tumor and its microenvironment. We first trained a multitask deep-learning neural network for automated single-cell segmentation and c...
Ayuso, Miriam Van Cruchten, Steven Van Ginneken, Chris
Published in
International journal of molecular sciences
The feed industry continuously seeks new molecules with antioxidant capacity since oxidative stress plays a key role in intestinal health. To improve screening of new antioxidants, this study aims to set up an assay to assess oxidative stress in the porcine small intestinal epithelial cell line IPEC-J2 using plate-reader-based analysis of fluoresce...
Qiao, Jiayun Sun, Zeyang Liang, Dongmei Li, Haihua
Published in
Journal of Animal Science and Biotechnology
BackgroundEnterotoxigenic Escherichia coli (ETEC) K88 commonly colonize in the small intestine and keep releasing enterotoxins to impair the intestinal barrier function and trigger inflammatory reaction. Although Lactobacillus salivarius (L. salivarius) has been reported to enhance intestinal health, it remains to be seen whether there is a functio...
van der Hee, Bart Madsen, Ole Vervoort, Jacques Smidt, Hauke Wells, Jerry M
Published in
Frontiers in cell and developmental biology
The emergence of intestinal organoids, as a stem cell-based self-renewable model system, has led to many studies on intestinal development and cell-cell signaling. However, potential issues regarding the phenotypic stability and reproducibility of the methodology during culture still needs to be addressed for different organoids. Here we investigat...
Dong, Na Li, Xinran Xue, Chenyu Zhang, Lei Wang, Chensi Xu, Xinyao Shan, Anshan
Published in
Journal of cellular physiology
Early weaning usually causes intestinal disorders, enteritis, and diarrhea in young animals and human infants. Astragalus polysaccharides (APS) possesses anti-inflammatory activity. To study the anti-inflammatory mechanisms of APS and its potential effects on intestinal health, we performed an RNA sequencing (RNA-seq) study in lipopolysaccharide (L...
Grześkowiak, Łukasz Pieper, Robert Kröger, Susan Martínez-Vallespín, Beatriz Hauser, Anja E. Niesner, Raluca Vahjen, Wilfried Zentek, Jürgen
Published in
Microorganisms
Clostridioides difficile toxins are one of the main causative agents for the clinical symptoms observed during C. difficile infection in piglets. Porcine milk has been shown to strengthen the epithelial barrier function in the piglet’s intestine and may have the potential to neutralise clostridial toxins. We hypothesised that porcine colostrum exer...
Madsen, Ole Vervoort, Jacques Smidt, Hauke Wells, Jerry M.
The emergence of intestinal organoids, as a stem cell-based self-renewable model system, has led to many studies on intestinal development and cell-cell signaling. However, potential issues regarding the phenotypic stability and reproducibility of the methodology during culture still needs to be addressed for different organoids. Here we investigat...
Ji, Xu Zheng, Weijiang Yao, Wen
Published in
Toxins
To explore the protective role of hydrogen gas (H2) on oxidative damage and apoptosis in intestinal porcine epithelial cells (IPEC-J2) induced by deoxynivalenol (DON), cells were assigned to four treatment groups, including control, 5 μM DON, H2-saturated medium, and 5 μM DON + H2-saturated medium treatments. After 12 h of different treatments, the...
Wang, Xichun Zhang, Yafei Zhao, Jie Cao, Li Zhu, Lei Huang, Yingying Chen, Xiaofang Rahman, Sajid Ur Feng, Shibin Li, Yu
...
Published in
Toxins
The aim of this study was to investigate the effects of deoxynivalenol (DON) exposure on the inflammatory injury nuclear factor kappa-B (NF-κB) pathway in intestinal epithelial cells (IPEC-J2 cells) of pig. The different concentrations of DON (0, 125, 250, 500, 1000, 2000 ng/mL) were added to the culture solution for treatment. The NF-κB pathway in...