Bakshi, Rachit Prakash, Tulika Dash, Debasis Brahmachari, Vani
Published in
Biochemical and Biophysical Research Communications
Proteins belonging to SNF2 family of DNA dependent ATPases are important members of the chromatin remodeling complexes that are implicated in epigenetic control of gene expression. The yeast Ino80, the catalytic ATPase subunit of the INO80 complex, is the most recently described member of the SNF2 family. Outside the conserved ATPase domain, it has...
Ford, Jason Odeyale, Oluwafemi Eskandar, Antonious Kouba, Nafila Shen, Chang-Hui
Published in
Biochemical and Biophysical Research Communications
Transcriptional activation in yeast INO1 chromatin was studied using the indirect end-labeling technique. INO1 chromatin is organized into an ordered, overlapping nucleosomal array under repressing conditions. Nucleosome positions were only disrupted at the promoter region under inducing conditions in the presence of SWI/SNF and INO80. Mutants lack...
Kawashima, Satoshi Ogiwara, Hideaki Tada, Shusuke Harata, Masahiko Wintersberger, Ulrike Enomoto, Takemi Seki, Masayuki
Published in
Biochemical and Biophysical Research Communications
The budding yeast INO80 complex has a role in remodeling chromatin structure, and the SWR1 complex replaces a H2A/H2B dimer with a variant dimer, H2A.Z (Htz1)/H2B. It has been reported that these chromatin remodeling complexes contain Arp4 (actin-related protein) and actin in common and are recruited to HO endonuclease-induced DNA double-strand bre...
Gangaraju, Vamsi K Bartholomew, Blaine
Published in
Mutation research
The inter-relationship between DNA repair and ATP dependent chromatin remodeling has begun to become very apparent with recent discoveries. ATP dependent remodeling complexes mobilize nucleosomes along DNA, promote the exchange of histones, or completely displace nucleosomes from DNA. These remodeling complexes are often categorized based on the do...
Bao, Yunhe Shen, Xuetong
Published in
Mutation research
ATP-dependent chromatin remodeling complexes contain ATPases of the Swi/Snf superfamily and alter DNA accessibility of chromatin in an ATP-dependent manner. Recently characterized INO80 and SWR1 complexes belong to a subfamily of these chromatin remodelers and are characterized by a split ATPase domain in the core ATPase subunit and the presence of...
van Attikum, Haico Fritsch, Olivier Gasser, Susan M
Published in
The EMBO journal
INO80 and SWR1 are two closely related ATP-dependent chromatin remodeling complexes that share several subunits. Ino80 was reported to be recruited to the HO endonuclease-induced double-strand break (DSB) at the budding yeast mating-type locus, MAT. We find Swr1 similarly recruited in a manner dependent on the phosphorylation of H2A (gammaH2AX). Th...
Farrants, Ann-Kristin Ostlund
Published in
FEBS letters
Chromatin remodelling is a prerequisite for nuclear processes, and cells have several different ways of remodelling the chromatin structure. The ATP-dependent chromatin remodelling complexes are large multiprotein complexes that use ATP to change DNA-histone contacts. These complexes are classified into 4 sub-families depending on the central ATPas...
Ford, Jason Odeyale, Oluwafemi Shen, Chang-Hui
Published in
Biochemical and Biophysical Research Communications
Transcriptional activation of yeast INO1 requires SWI/SNF and INO80 for nucleosome disruption at the promoter. However, the cooperative interplay among remodelers and their recruitment dynamics in activation have thus far been vague. Here, we showed, using chromatin immunoprecipitation, that both SWI/SNF and INO80 are present at the promoter and ar...
Meagher, Richard B. Kandasamy, Muthugapatti K. McKinney, Elizabeth C. Roy, Eileen
Published in
International Review of Cell and Molecular Biology
The nuclear actin-related proteins (ARPs) share overall structure and low-level sequence homology with conventional actin. They are indispensable subunits of macromolecular machines that control chromatin remodeling and modification leading to dynamic changes in DNA structure, transcription, and DNA repair. Cellular, genetic, and biochemical studie...
Sinha, Manisha Peterson, Craig L
Published in
Epigenomics
The integrity of a eukaryotic genome is often challenged by DNA double-strand breaks (DSBs). Even a single, unrepaired DSB can be a lethal event, or such unrepaired damage can result in chromosomal instability and loss of genetic information. Furthermore, defects in the pathways that respond to and repair DSBs can lead to the onset of several human...
