Poli, Jérôme Gerhold, Christian-Benedikt Tosi, Alessandro Hustedt, Nicole Seeber, Andrew Sack, Ragna Herzog, Franz Pasero, Philippe Shimada, Kenji Hopfner, Karl-Peter
...
Published in
Genes & development
Little is known about how cells ensure DNA replication in the face of RNA polymerase II (RNAPII)-mediated transcription, especially under conditions of replicative stress. Here we present genetic and proteomic analyses from budding yeast that uncover links between the DNA replication checkpoint sensor Mec1-Ddc2 (ATR-ATRIP), the chromatin remodeling...
Serber, Daniel W Runge, John S Menon, Debashish U Magnuson, Terry
Published in
Biology of reproduction
The ability to faithfully transmit genetic information across generations via the germ cells is a critical aspect of mammalian reproduction. The process of germ cell development requires a number of large-scale modulations of chromatin within the nucleus. One such occasion arises during meiotic recombination, when hundreds of DNA double-strand brea...
Hinz, John M Czaja, Wioletta
Published in
DNA repair
Base Excision Repair (BER) is a conserved, intracellular DNA repair system that recognizes and removes chemically modified bases to insure genomic integrity and prevent mutagenesis. Aberrant BER has been tightly linked with a broad spectrum of human pathologies, such as several types of cancer, neurological degeneration, developmental abnormalities...
Alatwi, Hanan E Downs, Jessica A
Published in
EMBO reports
The mammalian INO80 remodelling complex facilitates homologous recombination (HR), but the mechanism by which it does this is unclear. Budding yeast INO80 can remove H2A.Z/H2B dimers from chromatin and replace them with H2A/H2B dimers. H2A.Z is actively incorporated at sites of damage in mammalian cells, raising the possibility that H2A.Z may need ...
Volkman, Loy E
Negatively-supercoiled-ds DNA molecules, including the genomes of baculoviruses, spontaneously wrap around cores of histones to form nucleosomes when present within eukaryotic nuclei. Hence, nucleosome management should be essential for baculovirus genome replication and temporal regulation of transcription, but this has not been documented. Nucleo...
Kapoor, Prabodh Shen, Xuetong
Published in
Trends in Cell Biology
The mystery of nuclear actin has puzzled biologists for decades largely due to the lack of defined experimental systems. However, the development of actin-containing chromatin-modifying complexes as a defined genetic and biochemical system in the past decade has provided an unprecedented opportunity to dissect the mechanism of actin in the nucleus....
Bartholomew, Blaine
Published in
Annual review of biochemistry
A large family of chromatin remodelers that noncovalently modify chromatin is crucial in cell development and differentiation. They are often the targets of cancer, neurological disorders, and other human diseases. These complexes alter nucleosome positioning, higher-order chromatin structure, and nuclear organization. They also assemble chromatin,...
Seeber, Andrew Dion, Vincent Gasser, Susan M
Published in
Genes & development
Double-strand break repair by recombination requires a homology search. In yeast, induced breaks move significantly more than undamaged loci. To examine whether DNA damage provokes an increase in chromatin mobility generally, we tracked undamaged loci under DNA-damaging conditions. We found that the yeast checkpoint factors Mec1, Rad9, and Rad53 ar...
Hong, E.-J.E. Peterson, C.L.
Published in
Encyclopedia of Biological Chemistry
A eukaryotic genome is organized into nucleosomes, the repeating unit of chromatin. Although nucleosomes provide safe storage of long strands of DNA, the positional stability of nucleosomes inhibits access of the underlying DNA for transcription, repair of DNA damage, and DNA replication. Cells utilize three strategies to overcome this inhibition: ...
Olufemi, L. Bartholomew, B.
Published in
Brenner’s Encyclopedia of Genetics
DNA is organized into nucleosomes consisting of 147 bps of DNA wrapped around a protein spool. This organization is repeated genome-wide forming arrays of nucleosomes that interact with each other to form chromatin fibers. This organization regulates access to the genetic information. Special enzymes called adenosine triphosphate (ATP) dependent ch...
