Moustafa, Rehab
Hepatitis C virus is currently estimated to infect around 71 million people around the world. However, recent advances in drug development led to the generation of pangenotypic direct acting antivirals (DAA), which may make it possible to eliminate HCV by 2030 as planned by the World health organization (WHO). HCV is a small RNA enveloped virus of ...
Yusuff, Shamila Kurath, Gael Kim, Min Sun Tesfaye, Tarin M. Li, Jie McKenney, Douglas G. Vakharia, Vikram N.
Published in
Virology Journal
BackgroundViral hemorrhagic septicemia virus (VHSV), a fish rhabdovirus belonging to the Novirhabdovirus genus, causes severe disease and mortality in many marine and freshwater fish species worldwide. VHSV isolates are classified into four genotypes and each group is endemic to specific geographic regions in the north Atlantic and Pacific Oceans. ...
Kelly, Sean D. Opell, Brent D. Owens, Lindsey L.
Published in
The Science of Nature
Orb weavers produce webs that trap prey using a capture spiral formed of regularly spaced glue droplets supported by protein fibers. Each droplet consists of an outer aqueous layer and an adhesive, viscoelastic glycoprotein core. Organic and inorganic compounds in the aqueous layer make droplets hygroscopic and cause droplet features to change with...
Lin, Chi-Hung Peterson, Robyn A Gueniche, Audrey de Beaumais, Ségolène Adam Hourblin, Virginie Breton, Lionel Dalko, Maria Packer, Nicolle H
Published in
Microbiological research
Sweat is a secretory fluid that can be a source of unpleasant body odour due to interaction of resident bacteria with sweat components. Identification of glycoproteins in sweat suggests that protein-conjugated glycans may act as binding epitopes for bacteria, as found in other secretory fluids such as human milk, tears and saliva which help to prot...
Xu, Ganfei Wang, Jingjing Yin, Qiang Fang, Wei Xiao, Yazhong Fang, Zemin
Published in
Protein expression and purification
The fungal laccase Lcc9 from Coprinopsis cinerea is a promising candidate for biotechnological applications due to its distinct biochemical properties. In the present work, Lcc9 cDNA was cloned from C. cinerea using reverse transcription polymerase chain reaction and heterologously expressed in Pichia pastoris GS115. The recombinant laccase was fou...
Bando-Campos, Giroshi Juárez-López, Daniel Román-González, Sergio A. Castillo-Rodal, Antonia I. Olvera, Clarita López-Vidal, Yolanda Arreguín-Espinosa, Roberto Espitia, Clara Trujillo-Roldán, Mauricio A. Valdez-Cruz, Norma A.
...
Published in
Microbial Cell Factories
BackgroundPichia pastoris (syn. Komagataella phaffii) is one of the most highly utilized eukaryotic expression systems for the production of heterologous glycoproteins, being able to perform both N- and O-mannosylation. In this study, we present the expression in P. pastoris of an O-mannosylated recombinant version of the 38 kDa glycolipoprotein Ps...
Shiraishi, Takehiro Shinto, Eiji Mochizuki, Satsuki Tsuda, Hitoshi Kajiwara, Yoshiki Okamoto, Koichi Einama, Takahiro Hase, Kazuo Ueno, Hideki
Published in
Virchows Archiv
Mesothelin (MSLN) is a cell-surface glycoprotein present on mesothelial cells and in many cancers, where expression is generally associated with an unfavorable prognosis. The clinical significance and pathological characteristics of MSLN expression were evaluated by immunohistochemical staining of tissues from 530 stage ΙΙ/ΙΙΙ colorectal cancer (CR...
Zacchi, Lucía F Schulz, Benjamin L
Published in
Methods in molecular biology (Clifton, N.J.)
Glycosylation is a complex posttranslational modification that is critical for regulating the functions of diverse proteins. Analysis of protein glycosylation is made challenging by the high degree of heterogeneity in both glycan occupancy and structure. Here, we describe methods for data-independent acquisition (SWATH) mass spectrometry analysis o...
Chen, Fang Giang, Erick Gopal, Radhika Law, Mansun
Published in
Methods in molecular biology (Clifton, N.J.)
The hepatitis C virus (HCV) envelope glycoproteins, E1 and E2, are crucial for HCV assembly and entry, and are promising vaccine antigens. However, they are challenging to study because of technical difficulties in protein production and in quality control for protein folding and glycosylation. To study E1 and E2 in different experimental systems, ...
Barrios-Romero, María Del Mar Crevillén, Agustín G Puerta, Angel de Frutos, Mercedes Diez-Masa, José Carlos
Published in
Methods in molecular biology (Clifton, N.J.)
In the last few years, biopharmaceuticals-therapeutic drugs which are generally obtained by using molecular biology techniques-have become a major growing sector in pharmaceutical industry. A large part of these biopharmaceuticals are therapeutic glycoproteins. The production of these drugs and their purification process are implying the developmen...