Published in Microchimica Acta
A non-enzymatic fluorometric assay is described for the determination of glucose. The method is based on the use of g-C3N4 quantum dots (QDs) that have good water solubility. The QDs were synthesized by a one-step solvothermal process using formamide as precursor. The QDs possess an average size of ~5 nm, a band gap of 3.0~3.5 eV, and strong blue f...
Published in Microchimica Acta
A luminescent metal organic framework (LMOF) of type UiO-66-NH2 was chosen for specific and sensitive detection of trace levels of hypochlorite. Hypochlorite causes the quenching of the blue fluorescence of nano-UiO-66-NH2 (with excitation/emission maxima at 325/430 nm), and this finding forms the basis for a fluorometric assay for hypochlorite. Th...
Published in Microchimica Acta
Pyrophosphate-modified carbon quantum dots (PP-CDs) are demonstrated to be a viable fluorescent nanoprobe for mercury(II) (Hg2+) detection. Hg2+ reacts with the pyrophosphate groups on the surface of PP-CDs to form a non-fluorescent complex. This results in quenching of the green fluorescence which has excitation/emission peaks at 400/513 nm. Stati...
Published in Microchimica Acta
A fluorometric aptamer based assay is described for rapid and sensitive detection of aflatoxin B1 (AFB1). It is making use of a fluorescein (FAM) labeled anti-AFB1 aptamer and complementary DNA-modified gold nanoparticles (GNPs). In the absence of AFB1, the FAM-labeled aptamers hybridize with complementary DNA strands that were covalently immobiliz...
Published in Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
Polythioether has good chemical stability and biocompatibility and is a kind of promising polymers for the application of optical materials, medical materials and energy conversion materials. However, the fluorescent probe based on polythioether is still rare. Herein, a series of polythioether based polymer fluorescent probes were synthesized by su...
Published in Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
Herein, a facile and quick strategy to detect thiourea was conducted based on the reversion of fluorescence quenching of nitrogen doped carbon dots (NCDs) by Hg2+. The NCDs with good water solubility and 17% of quantum yield was synthesized by one-step hydrothermal method, using ammonium citrate and dextrin as carbon source and nitrogen source, res...
Published in Journal of molecular biology
Rho-dependent transcription termination is a well-conserved process in bacteria. The Psu and YaeO proteins are the two established inhibitors of the ATP-dependent RNA helicase Rho protein of Escherichia coli. Here, we show a detailed sequence and phylogenetic analysis demonstrating that Vibrio cholerae YaeO (VcYaeO) is significantly distinct from i...
Published in Ecotoxicology and environmental safety
The mechanism of improving pyrene (PYR)-degrading ability of bacteria CP13 in Layer-by-layer (LBL) assembly chitosan/alginate (CHI/ALG) bio-microcapsules was investigated. Flow cytometry analysis showed that LBL microcapsules could effectively slow down the increasing rate of bacterial cell membrane permeability and the decreasing rate of the membr...
Published in International journal of biological macromolecules
Binding of anticancer drug altretamine with bovine serum albumin (BSA) and its inhibitory effect on fibrillation of the protein has been studied by using a combination of spectroscopic and calorimetric methods. Altretamine is observed to bind with BSA with a moderate binding affinity of the order of 105, which is weakly temperature dependent. Circu...
Published in Microchimica Acta
A fluorescent probe is described for detection of mercury(II) ion by using L-cysteine-modified gold nanoparticles (Cys-AuNP). These were fabricated by a tube-based redox reaction where Cys acts as both the reducing reagent and capping ligand. The Cys-AuNP display red fluorescence, with excitation/emission peaks at 373/625 nm. Owing to the high-affi...
