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A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry.

Published in Journal of visualized experiments : JoVE

Many biological cells/tissues sense the mechanical properties of their local environments via mechanoreceptors, proteins that can respond to forces like pressure or mechanical perturbations. Mechanoreceptors detect their stimuli and transmit signals via a great diversity of mechanisms. Some of the most common roles for mechanoreceptors are in neuro...

FZD5 (frizzled class receptor 5)

The Wnt signalling pathways are ways of signalling that transduce signals into cells through cell surface receptors; they are three different type of pathways and all of these are activated by binding a Wnt-protein ligand to a Frizzled family receptor. Frizzled is a family of G-protein coupled receptor proteins that works as receptor in the Wnt sig...

Leukemia Cell Cycle Chemical Profiling Identifies the G2-Phase Leukemia Specific Inhibitor Leusin-1.

Targeting the leukemia proliferation cycle has been a successful approach to developing antileukemic therapies. However, drug screening efforts to identify novel antileukemic agents have been hampered by the lack of a suitable high-throughput screening platform for suspension cells that does not rely on flow-cytometry analyses. We report the develo...

Scaled-Up Inertial Microfluidics: Retention System for Microcarrier-Based Suspension Cultures

© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Recently, particle concentration and filtration using inertial microfluidics have drawn attention as an alternative to membrane and centrifugal technologies for industrial applications, where the target particle size varies between 1 µm and 500 µm. Inevitably, the bigger particle size (>50 µm) manda...

t(6;8)(p21;q24) MYC/SUPT3H

Review on t(6;8)(p21;q24), with data on clinics, and the genes involved.

A novel fluorescent surfactant enhances the delivery of the Cas9 ribonucleoprotein and enables the identification of edi...

In this report, we designed and synthesized a novel fluorescent single tailed surfactant (termed FEDS), which can disrupt endosomes, complex lipofectamine, and can also identify cells that have been transfected. FEDS was able to increase the gene editing efficiency of lipofectamine/Cas9 ribonucleoprotein by 300% via a combination of fluorescent bas...

Robust prediction of clinical outcomes using cytometry data.

MotivationFlow cytometry and mass cytometry are widely used to diagnose diseases and to predict clinical outcomes. When associating clinical features with cytometry data, traditional analysis methods require cell gating as an intermediate step, leading to information loss and susceptibility to batch effects. Here, we wish to explore an alternative ...