Iida, T. Kuwata, T. Ui, M. Suzuki, H. Miura, T. Ibuki, K. Takahashi, H. Yamamoto, T. Imanishi, J. Hayami, M.
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Published in
Archives of Virology
A nef-deleted SHIV-NM-3rN (SHIV-NI) was previously shown to be nonpathogenic and to induce protective immunity. In the present study, a SHIV-NI expressing human interferon-γ (SHIV-IFN-γ) was constructed and the effect of co-expression of IFN-γ on virus replication and immunopotentiation was investigated in macaques that were vaccinated with both vi...
Roose, Jeroen P Diehn, Maximilian Tomlinson, Michael G Lin, Joseph Alizadeh, Ash A Botstein, David Brown, Patrick O Weiss, Arthur
Signal transduction pathways guided by cellular receptors commonly exhibit low-level constitutive signaling in a continuous, ligand-independent manner. The dynamic equilibrium of positive and negative regulators establishes such a tonic signal. Ligand-independent signaling by the precursors of mature antigen receptors regulates development of B and...
5T4 oncotrophoblast antigen is a transmembrane glycoprotein expressed by trophoblast and many carcinomas but not most normal adult tissues. Results from overexpression of human and mouse 5T4 cDNA in cell lines are consistent with it having an influence on adhesion, shape and motility. We show that murine embryonic stem cell lines are 5T4 negative b...
Ogura, Y Ogasawara, N Harry, EJ Moriya, S
The ParA and ParB protein families are well conserved in bacteria. However, their functions are still unclear. In Bacillus subtilis, Soj and Spo0J are members of these two protein families, respectively. A previous report revealed that replication initiated early and asynchronously in spo0J null mutant cells, as determined by flow cytometry. In thi...
Collingridge, D R Glaser, M Osman, S Barthel, H Hutchinson, O C Luthra, S K Brady, F Bouchier-Hayes, L Martin, S J Workman, P
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Published in
British journal of cancer
The availability of a noninvasive method to detect and quantify apoptosis in tumours will enable tumour response to several cancer therapies to be assessed. We have synthesised two radiotracers, annexin V and the N-succinimidyl-3-iodobenzoic acid (SIB) derivative of annexin V, labelled with radio-iodine ((124)I and (125)I) and provided proof of the...
La, Mylinh Cao, Thong V. Cerchiaro, Graziela Chilton, Kathya Hirabayashi, Jun Kasai, Ken-ichi Oliani, Sonia M. Chernajovsky, Yuti Perretti, Mauro
Published in
The American Journal of Pathology
Galectin-1 (Gal-1), the prototype of a family of β-galactoside-binding proteins, has been shown to attenuate experimental acute and chronic inflammation. In view of the fact that endothelial cells (ECs), but not human polymorphonuclear leukocytes (PMNs), expressed Gal-1 we tested here the hypothesis that the protein could modulate leukocyte-EC inte...
Ormerod, Michael G.
Published in
Human Genetics
Sørensen, Søren J. Sørensen, Anders H. Hansen, Lars H. Oregaard, Gunnar Veal, Duncan
Published in
Current Microbiology
A new cultivation-independent method for studying conjugal gene transfer between bacteria was evaluated. The method was based on direct detection and enumeration of donor and transconjugant bacterial cells by flow cytometry. Specific detection of transconjugants was obtained by using a conjugative plasmid tagged with a reporter gene (gfp) encoding ...
Abaza, Aouatef Soleilhac, Jean-Marc Westendorf, Joanne Piel, Matthieu Crevel, Isabelle Roux, Aurelien Pirollet, Fabienne
Published in
The Journal of biological chemistry
The human M phase phosphoprotein 1 (MPP1), previously identified through a screening of a subset of proteins specifically phosphorylated at the G2/M transition (Matsumoto-Taniura, N., Pirollet, F., Monroe, R., Gerace, L., and Westendorf, J. M. (1996) Mol. Biol. Cell 7, 1455-1469), is characterized as a plus-end-directed kinesin-related protein. Rec...
Aguedo, Mario Waché, Yves Mazoyer, Virginie Sequeira-Le Grand, Anabelle Belin, Jean-Marc
Published in
Journal of agricultural and food chemistry
The adhesion of methyl ricinoleate droplets to cells of the yeast Yarrowia lipolytica was investigated. A new cytometric method, relying on the double staining of fatty globules with Nile Red and of cells with Calcofluor, enabled us to quantify methyl ricinoleate droplet adhesion to cells precultured on a hydrophilic or on a hydrophobic carbon sour...
