Gehringer, Christian Regeniter, Axel Rentsch, Katharina Tschudin-Sutter, Sarah Bassetti, Stefano Egli, Adrian
Published in
BMC Infectious Diseases
Background Urinary tract infection (UTI) is diagnosed combining urinary symptoms with demonstration of urine culture growth above a given threshold. Our aim was to compare the diagnostic accuracy of Urine Flow Cytometry (UFC) with urine test strip in predicting bacterial growth and in identifying contaminated urine samples, and to derive an algorit...
Gupta, Monika Monga, Lovekesh Mehrotra, Dimple Chhabra, Sonia Singhal, Shivani Sen, Rajeev
Published in
Oman Medical Journal
Objectives Acute leukemias (AL) are a heterogeneous group of hematological malignancies with the presence of 20% or more blasts in the peripheral blood or bone marrow. Malignant cells display characteristic patterns of surface antigenic expression. Aberrant phenotypes are defined as patterns of antigen expression on neoplastic cells different from ...
Aghajari, Sima Mortazavi, Sayed Mohammad Javad Kalani, Mehdi Nematolahi, Samaneh Habibzadeh, Parham Farjadian, Shirin
Published in
Cell journal
Astronauts are exposed to a wide range of environmental stresses during spaceflights that reduce their immune responses and make them more susceptible to infections and malignancies. Exposure to a low dose of a certain stress induces an adaptive response, which leads to resistance to higher doses of the same or other types of stress. We designed th...
Zingaropoli, Maria Antonella Perri, Valentina Pasculli, Patrizia Cogliati Dezza, Francesco Nijhawan, Parni Savelloni, Giulia La Torre, Giuseppe D'Agostino, Claudia Mengoni, Fabio Lichtner, Miriam
...
Published in
Clinical Immunology (Orlando, Fla.)
• High percentages of NK CD57+ cells and CD56dimNK cells in COVID-19 subjects • Low percentages of CD56bright and NKT cells in COVID-19 subjects • Severe COVID-19 pneumonia and NKT cell reduction were independently associated
Liu, Lei Yu, Ying Peng, Qiuyue Porsborg, Simone R Nielsen, Frederik M Jørgensen, Annemette Grove, Anni Bath, Chris Hjortdal, Jesper Christiansen, Ole B
...
Published in
Journal of Ophthalmic & Vision Research
Purpose To reveal the phenotypic differences between human ocular surface stromal cells (hOSSCs) cultured from the corneal, limbal, and scleral compartments. Methods A comparative analysis of cultured hOSSCs derived from four unrelated donors was conducted by multichromatic flow cytometry for six distinct CD antigens, including the CD73, CD90, CD10...
Busuttil-Crellin, Xavier McCafferty, Conor Van Den Helm, Suelyn Yaw, Hui Ping Monagle, Paul Linden, Matthew Ignjatovic, Vera
Published in
Platelets
Flow cytometry is a valuable tool in determining the phenotype and function of platelets accurately. The emergence of platelet flow cytometry in recent years provides an attractive alternative to other platelet analytical techniques, with advantages such as requiring small volumes and being highly sensitive to minimal changes in receptor function a...
Sato, Hanae Singer, Robert H. Greally, John M.
Published in
Bio-protocol
Dynamic histone changes occur as a central part of chromatin regulation. Deposition of histone variants and post-translational modifications of histones are strongly associated with properties of chromatin status. Characterizing the kinetics of histone variants allows important insights into transcription regulation, chromatin maintenance and other...
Ramesh, Akshaya Schubert, Ryan D Greenfield, Ariele L Dandekar, Ravi Loudermilk, Rita Sabatino, Joseph J Koelzer, Matthew T Tran, Edwina B Koshal, Kanishka Kim, Kicheol
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Central nervous system B cells have several potential roles in multiple sclerosis (MS): secretors of proinflammatory cytokines and chemokines, presenters of autoantigens to T cells, producers of pathogenic antibodies, and reservoirs for viruses that trigger demyelination. To interrogate these roles, single-cell RNA sequencing (scRNA-Seq) was perfor...
Hu, Zicheng Tang, Alice Singh, Jaiveer Bhattacharya, Sanchita Butte, Atul J
Cytometry technologies are essential tools for immunology research, providing high-throughput measurements of the immune cells at the single-cell level. Existing approaches in interpreting and using cytometry measurements include manual or automated gating to identify cell subsets from the cytometry data, providing highly intuitive results but may ...
Hammerle, Constanze M Sandovici, Ionel Brierley, Gemma Smith, Nicola M Zimmer, Warren E Zvetkova, Ilona Prosser, Haydn M Sekita, Yoichi Lam, Brian YH Ma, Marcella
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The genetic mechanisms that determine the size of the adult pancreas are poorly understood. Imprinted genes, which are expressed in a parent-of-origin-specific manner, are known to have important roles in development, growth and metabolism. However, our knowledge regarding their roles in the control of pancreatic growth and function remains limited...