Ooigawa, Hidetoshi Nawashiro, Hiroshi Fukui, Shinji Otani, Naoki Osumi, Atsushi Toyooka, Terushige Shima, Katsuji
Published in
Acta Neuropathologica
We studied the fate of Nissl-stained dark neurons (N-DNs) following traumatic brain injury (TBI). N-DNs were investigated in the cerebral neocortex and the hippocampus using a rat lateral fluid percussion injury model. Nissl stain, acid fuchsin stain and immunohistochemistry with phosphorylated extracellular signal-regulated protein kinase (pERK) a...
Liu, Hui-Qing Li, Wen-Bin Li, Qing-Jun Zhang, Min Sun, Xiao-Cai Feng, Rong-Fang Xian, Xiao-Hui Li, Shu-Qin Qi, Jie Zhao, Hong-Gang
...
Published in
Neurochemical Research
The present study was undertaken to observe in vivo changes of expression and phosphorylation of ERK1/2 proteins during brain ischemic preconditioning and effects of inhibiting generation of nitric oxide (NO) on the changes to determine the role of ERKs in the involvement of NO participating in the acquired tolerance. Fifty-five Wistar rats were us...
Duan, W. Wong, W.S.F.
10.2174/138945006777435353 / Current Drug Targets / 7 / 6 / 691-698
Jacques-Silva, Maria C. Bernardi, Andressa Rodnight, Richard Lenz, Guido
Published in
Oncology
Objective: Extracellular nucleotides and nucleosides induce proliferation in a set of human glioma cell lines. In this study we investigate the signal transduction pathways involved in ATP and adenosine-mediated proliferation in U138-MG human glioma cells. Methods: Cell proliferation was accessed through [3H]thymidine incorporation, cell counting a...
Bae, Yoe-Sik Park, Eun-Young Lee, Ha-Young Kang, Hyun Kyu Suh, Pann-Ghill Kwak, Jong-Young Ryu, Sung Ho Lee, Taehoon
Published in
Biochemical and Biophysical Research Communications
The screening of small synthetic compound libraries is a useful means of identifying molecules that modulate various cellular responses. We screened more than 10,000 different small compounds and identified three synthetic compounds that stimulate arachidonic acid (AA) release in a combinational manner in neutrophil-like differentiated HL60 cells. ...
Starkey, Dustin E. Abdelaziez, Yasser Ahn, Chong H. Tu, Jian Anderson, LaShonda Wehmeyer, Kenneth R. Izzo, Nicholas J. Carr, Andrew N. Peters, Kevin G. Bao, James J.
...
Published in
Analytical Biochemistry
The application of microchip capillary electrophoresis (CE) to the assay of extracellular signal-regulated protein kinase (ERK) is presented. In this assay, ERK catalyzes the transfer of γ-phosphate from adenosine 5 ′-triphosphate to the threonine residue of a fluorescently labeled nonapeptide (APRTPGGRR), and the phosphorylated and nonphosphorylat...
Huang, Jianhe Tu, Zheng Lee, Frank S
Published in
Biochemical and Biophysical Research Communications
MAPK/ERK kinase kinase 2 (MEKK2) is a member of the mitogen-activated protein kinase kinase kinase (MAP3K) family of protein kinases. MAP3Ks are components of a three-tiered protein kinase pathway in which a MAP3K phosphorylates and activates a mitogen-activated protein kinase kinase (MAP2K), which in turn activates a mitogen-activated protein kina...
Wong, C.K. Zhang, J.P. Lam, C.W.K. Ho, C.Y. Hjelm, N.M.
Published in
International Archives of Allergy and Immunology
Background: It has been shown that the inhibition of eosinophilic apoptosis is an important mechanism for the development of blood and tissue eosinophilia in allergic diseases. Considerable attention has recently been focused on the role played by different intracellular kinase cascades in the control of apoptosis. In the present study, we investig...
kim, hyae kyeong nam, youn han, young lee, eun kyung choi, jung bok, song hae kwon, byoung-mog
Actinomycins, a family of bicyclic chromopeptide lactones with strong antineoplastic activity, were screened as inhibitors of Shc/Grb2 interaction in in vitro assay systems. To investigate the effects of actinomycin D on Shc/Grb2 interaction in cell-based experiments, we used SAA (normal hEGFR-overexpressed NIH3T3) cells and B104-1-1 (neu*-transfor...
Hyae Kyeong Kim Ji Youn Nam Mi Young Han Eun Kyung Lee Jung Do Choi Song Hae Bok Byoung-Mog Kwon
Actinomycins, a family of bicyclic chromopeptide lactones with strong antineoplastic activity, were screened as inhibitors of Shc/Grb2 interaction in in vitro assay systems. To investigate the effects of actinomycin D on Shc/Grb2 interaction in cell-based experiments, we used SAA (normal hEGFR-overexpressed NIH3T3) cells and B104-1-1 (neu*-transfor...
