Pojero, Fanny Flores-Montero, Juan Sanoja, Luzalba Pérez, José Juan Puig, Noemí Paiva, Bruno Bottcher, Sebastian van Dongen, Jacques J M Orfao, Alberto
Published in
Cytometry. Part B, Clinical cytometry
Multiparameter flow cytometry (MFC) identification and characterization of plasma cells (PCs) is a useful tool to support diagnosis, prognostication, and monitoring of PC diseases (PCD). Currently, the number of MFC markers suited for the identification of PC remains limited. Moreover, antibody therapies against PC-associated markers further compro...
Flores-Montero, Juan de Tute, Ruth Paiva, Bruno Perez, José Juan Böttcher, Sebastian Wind, Henk Sanoja, Luzalba Puig, Noemí Lecrevisse, Quentin Vidriales, María Belén
...
Published in
Cytometry. Part B, Clinical cytometry
In recent years, several studies on large series of multiple myeloma (MM) patients have demonstrated the clinical utility of flow cytometry monitoring of minimal residual disease (flow-MRD) in bone marrow (BM), for improved assessment of response to therapy and prognostication. However, disturbing levels of variability exist regarding the specific ...
Nováková, Michaela Glier, Hana Brdičková, Naděžda Vlková, Marcela Santos, Ana Helena Lima, Margarida Roussel, Mikael Flores-Montero, Juan Szczepanski, Tomasz Böttcher, Sebastian
...
Published in
Journal of immunological methods
A critical component of the EuroFlow standardization of leukemia/lymphoma immunophenotyping is instrument setup. Initially, the EuroFlow consortium developed a step-by-step standard operating protocol for instrument setup of ≥8-color flow cytometers that were available in 2006, when the EuroFlow activities started. Currently, there are 14 instrumen...
van Dongen, Jacques J. M. van der Burg, Mirjam Kalina, Tomas Perez-Andres, Martin Mejstrikova, Ester Vlkova, Marcela Lopez-Granados, Eduardo Wentink, Marjolein Kienzler, Anne-Kathrin Philippé, Jan
...
Published in
Frontiers in Immunology
Guidelines for screening for primary immunodeficiencies (PID) are well-defined and several consensus diagnostic strategies have been proposed. These consensus proposals have only partially been implemented due to lack of standardization in laboratory procedures, particularly in flow cytometry. The main objectives of the EuroFlow Consortium were to ...
van der Burg, Mirjam Kalina, Tomas Perez-Andres, Martin Vlkova, Marcela Lopez-Granados, Eduardo Blanco, Elena Bonroy, Carolien Sousa, Ana E. Kienzler, Anne-Kathrin Wentink, Marjolein
...
Published in
Frontiers in Immunology
In the rapidly evolving field of primary immunodeficiencies (PID), the EuroFlow consortium decided to develop a PID orientation and screening tube that facilitates fast, standardized, and validated immunophenotypic diagnosis of lymphoid PID, and allows full exchange of data between centers. Our aim was to develop a tool that would be universal for ...
Takamatsu, Hiroyuki Yoroidaka, Takeshi Fujisawa, Momoko Kobori, Kazuya Hanawa, Masako Yamashita, Takeshi Murata, Ryoichi Ueda, Mikio Nakao, Shinji Matsue, Kosei
...
Published in
International Journal of Hematology
We sought to determine the efficacy of a new, inexpensive, single-tube 8-color multiparameter flow cytometry (MFC) method (SRL-Flow), which is based on the EuroFlow next-generation flow (NGF) (tube 2 only), to assess minimal residual disease (MRD)-negative status. MRD-negative status is considered a treatment milestone in multiple myeloma (MM). We ...
Edwards, Emily S. J. Bosco, Julian J. Aui, Pei M. Stirling, Robert G. Cameron, Paul U. Chatelier, Josh Hore-Lacy, Fiona O'Hehir, Robyn E. van Zelm, Menno C.
Published in
Frontiers in Immunology
Background: Patients with predominantly antibody deficiency (PAD) suffer from severe and recurrent infections that require lifelong immunoglobulin replacement and prophylactic antibiotic treatment. Disease incidence is estimated to be 1:25,000 worldwide, and up to 68% of patients develop non-infectious complications (NIC) including autoimmunity, wh...
Kalina, Tomas Brdickova, Nadezda Glier, Hana Fernandez, Paula Bitter, Marieke Flores-Montero, Juan van Dongen, Jacques J M Orfao, Alberto
Published in
Journal of immunological methods
EuroFlow Quality Assessment was designed to provide a feedback on the quality of the standardization effort in executing the EuroFlow protocols for sample preparation and instrument setup. It was first beta-tested by the members of the EuroFlow consortium internally (2010-2013) and opened to the external participants from 2015 onwards. The goal of ...
Blanco, Elena Perez-Andres, Martin Sanoja-Flores, Luzalba Wentink, Marjolein Pelak, Ondrej Martín-Ayuso, Marta Grigore, Georgiana Torres-Canizales, Juan López-Granados, Eduardo Kalina, Tomas
...
Published in
Journal of immunological methods
The clinical value of assessing immunoglobulin (Ig)G and IgA subclasses in addition to the isotypes of soluble Igs in serum has been well established. >20years ago, the International Union of Immunological Societies and the World Health Organization performed collaborative studies in order to validate antibody (Ab) clones for the detection of IgG a...
Glier, Hana Heijnen, Ingmar Hauwel, Mathieu Dirks, Jan Quarroz, Stéphane Lehmann, Thomas Rovo, Alicia Arn, Kornelius Matthes, Thomas Hogan, Cassandra
...
Published in
Journal of immunological methods
The EuroFlow Consortium developed a fully standardized flow cytometric approach from instrument settings, through antibody panel, reagents and sample preparation protocols, to data acquisition and analysis. The Swiss Cytometry Society (SCS) promoted a study to evaluate the feasibility of using such standardized measurements of 8-color data across t...
