Kalina, Tomas Bakardjieva, Marina Blom, Maartje Perez-Andres, Martin Barendregt, Barbara Kanderová, Veronika Bonroy, Carolien Philippé, Jan Blanco, Elena Pico-Knijnenburg, Ingrid
...
Published in
Frontiers in Immunology
The EuroFlow PID consortium developed a set of flow cytometry tests for evaluation of patients with suspicion of primary immunodeficiency (PID). In this technical report we evaluate the performance of the SCID-RTE tube that explores the presence of recent thymic emigrants (RTE) together with T-cell activation status and maturation stages and discus...
Kalina, Tomas
Published in
Cytometry. Part A : the journal of the International Society for Analytical Cytology
There is an agreement in the field that interlaboratory reproducibility of flow cytometry measurements as well as the whole studies might be improved by a consensual use of methodological approach. Typically, a consensus is made on a crucial markers needed in the immunostaining panel, sometimes on the particular fluorochrome conjugates and rarely o...
Nováková, Michaela Glier, Hana Brdičková, Naděžda Vlková, Marcela Santos, Ana Helena Lima, Margarida Roussel, Mikael Flores-Montero, Juan Szczepanski, Tomasz Böttcher, Sebastian
...
Published in
Journal of immunological methods
A critical component of the EuroFlow standardization of leukemia/lymphoma immunophenotyping is instrument setup. Initially, the EuroFlow consortium developed a step-by-step standard operating protocol for instrument setup of ≥8-color flow cytometers that were available in 2006, when the EuroFlow activities started. Currently, there are 14 instrumen...
Kalina, Tomas Brdickova, Nadezda Glier, Hana Fernandez, Paula Bitter, Marieke Flores-Montero, Juan van Dongen, Jacques J M Orfao, Alberto
Published in
Journal of immunological methods
EuroFlow Quality Assessment was designed to provide a feedback on the quality of the standardization effort in executing the EuroFlow protocols for sample preparation and instrument setup. It was first beta-tested by the members of the EuroFlow consortium internally (2010-2013) and opened to the external participants from 2015 onwards. The goal of ...
Blanco, Elena Perez-Andres, Martin Sanoja-Flores, Luzalba Wentink, Marjolein Pelak, Ondrej Martín-Ayuso, Marta Grigore, Georgiana Torres-Canizales, Juan López-Granados, Eduardo Kalina, Tomas
...
Published in
Journal of immunological methods
The clinical value of assessing immunoglobulin (Ig)G and IgA subclasses in addition to the isotypes of soluble Igs in serum has been well established. >20years ago, the International Union of Immunological Societies and the World Health Organization performed collaborative studies in order to validate antibody (Ab) clones for the detection of IgG a...
Glier, Hana Heijnen, Ingmar Hauwel, Mathieu Dirks, Jan Quarroz, Stéphane Lehmann, Thomas Rovo, Alicia Arn, Kornelius Matthes, Thomas Hogan, Cassandra
...
Published in
Journal of immunological methods
The EuroFlow Consortium developed a fully standardized flow cytometric approach from instrument settings, through antibody panel, reagents and sample preparation protocols, to data acquisition and analysis. The Swiss Cytometry Society (SCS) promoted a study to evaluate the feasibility of using such standardized measurements of 8-color data across t...
Edwards, Emily S. J. Bosco, Julian J. Aui, Pei M. Stirling, Robert G. Cameron, Paul U. Chatelier, Josh Hore-Lacy, Fiona O'Hehir, Robyn E. van Zelm, Menno C.
Published in
Frontiers in Immunology
Background: Patients with predominantly antibody deficiency (PAD) suffer from severe and recurrent infections that require lifelong immunoglobulin replacement and prophylactic antibiotic treatment. Disease incidence is estimated to be 1:25,000 worldwide, and up to 68% of patients develop non-infectious complications (NIC) including autoimmunity, wh...
van Dongen, Jacques J. M. van der Burg, Mirjam Kalina, Tomas Perez-Andres, Martin Mejstrikova, Ester Vlkova, Marcela Lopez-Granados, Eduardo Wentink, Marjolein Kienzler, Anne-Kathrin Philippé, Jan
...
Published in
Frontiers in Immunology
Guidelines for screening for primary immunodeficiencies (PID) are well-defined and several consensus diagnostic strategies have been proposed. These consensus proposals have only partially been implemented due to lack of standardization in laboratory procedures, particularly in flow cytometry. The main objectives of the EuroFlow Consortium were to ...
Takamatsu, Hiroyuki Yoroidaka, Takeshi Fujisawa, Momoko Kobori, Kazuya Hanawa, Masako Yamashita, Takeshi Murata, Ryoichi Ueda, Mikio Nakao, Shinji Matsue, Kosei
...
Published in
International Journal of Hematology
We sought to determine the efficacy of a new, inexpensive, single-tube 8-color multiparameter flow cytometry (MFC) method (SRL-Flow), which is based on the EuroFlow next-generation flow (NGF) (tube 2 only), to assess minimal residual disease (MRD)-negative status. MRD-negative status is considered a treatment milestone in multiple myeloma (MM). We ...
van der Burg, Mirjam Kalina, Tomas Perez-Andres, Martin Vlkova, Marcela Lopez-Granados, Eduardo Blanco, Elena Bonroy, Carolien Sousa, Ana E. Kienzler, Anne-Kathrin Wentink, Marjolein
...
Published in
Frontiers in Immunology
In the rapidly evolving field of primary immunodeficiencies (PID), the EuroFlow consortium decided to develop a PID orientation and screening tube that facilitates fast, standardized, and validated immunophenotypic diagnosis of lymphoid PID, and allows full exchange of data between centers. Our aim was to develop a tool that would be universal for ...
