Delgado, Alejandro H. Fluxa, Rafael Perez-Andres, Martin Diks, Annieck M. van Gaans-van den Brink, Jacqueline A. M... Barkoff, Alex-Mikael Blanco, Elena Torres-Valle, Alba Berkowska, Magdalena A. Grigore, Georgiana
...
Published in
Frontiers in Immunology
Background Multiparameter flow cytometry (FC) immunophenotyping is a key tool for detailed identification and characterization of human blood leucocytes, including B-lymphocytes and plasma cells (PC). However, currently used conventional data analysis strategies require extensive expertise, are time consuming, and show limited reproducibility. Obje...
e., on of on of anne
Flowcytometric analysis allows for detailed identification and characterization of large numbers of cells in blood, bone marrow, and other body fluids and tissue samples and therefore contributes to the diagnostics of hematological malignancies. Novel data analysis tools allow for multidimensional analysis and comparison of patient samples with ref...
Broijl, Annemiek de Jong, Augustinus C M van Duin, Mark Sonneveld, Pieter Kühnau, Jesper van der Velden, Vincent H J
Published in
American journal of clinical pathology
To compare flow cytometric minimal residual disease (MRD) data obtained using the EuroFlow approach, including the CD38-multiepitope (ME) antibody or the VS38c antibody. We evaluated 29 bone marrow samples from patients with multiple myeloma (MM), of whom 15 had received daratumumab within the past 6 months. We evaluated MRD data and fluorescence i...
brouwer;, on of on of nienke
Acute megakaryoblastic leukemia (AMKL) is a rare and heterogeneous subtype of acute myeloid leukemia (AML). We evaluated the immunophenotypic profile of 72 AMKL and 114 non-AMKL AML patients using the EuroFlow AML panel. Univariate and multivariate/multidimensional analyses were performed to identify most relevant markers contributing to the diagno...
Bras, Anne E. Matarraz, Sergio Nierkens, Stefan Fernández, Paula Philippé, Jan Aanei, Carmen-Mariana de Mello, Fabiana Vieira Burgos, Leire van der Sluijs-Gelling, Alita J. Grigore, Georgiana Emilia
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Simple Summary Flow cytometry allows detailed characterization of large numbers of cells and plays an important role in the diagnosis of acute myeloid leukemia. To facilitate analysis of flowcytometric data, reference databases of normal bone marrow samples and samples from acute myeloid leukemia patients, together with new software tools, are requ...
Brouwer, Nienke Matarraz, Sergio Nierkens, Stefan Hofmans, Mattias Nováková, Michaela da Costa, Elaine Sobral Fernandez, Paula Bras, Anne E. de Mello, Fabiana Vieira Mejstrikova, Ester
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Simple Summary Acute megakaryoblastic leukemia (AMKL) is a rare and heterogeneous subtype of acute myeloid leukemia (AML). We show that such patients can be identified by flowcytometric immunophenotyping using the standardized EuroFlow panel. AMKL patients show a unique immunophenotypic profile, and among AMKL patients, various subgroups can be dis...
Sato, Kota Okazuka, Kiyoshi Ishida, Tadao Sakamoto, Jun Kaneko, Shigeto Nashimoto, Junichiro Uto, Yui Ogura, Mizuki Yoshiki, Yumiko Abe, Yu
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Published in
Annals of hematology
Minimal residual disease (MRD)-negative status in multiple myeloma (MM) is associated with favorable outcomes. Although EuroFlow next-generation flow (NGF) is a global standard for MRD detection, its operating cost is high. Therefore, it is desirable to develop a less expensive method with equivalent sensitivity to that of EuroFlow-NGF. In this stu...
Kastritis, Esftathios Kostopoulos, Ioannis V Theodorakakou, Foteini Fotiou, Despina Gavriatopoulou, Maria Migkou, Magdalini Tselegkidi, Maria Irini Roussou, Maria Papathoma, Alexandra Eleutherakis-Papaioakovou, Evangelos
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Published in
Amyloid : the international journal of experimental and clinical investigation : the official journal of the International Society of Amyloidosis
The treatment of AL amyloidosis aims to eradicate the plasma cell clone and eliminate toxic free light chain production. Only in a minority of patients the plasma cell clone is completely eradicated; residual light chain production may still exist while clonal relapse may occur. We used sensitive next-generation flow cytometry (NGF) to detect minim...
Linskens, Eleni Diks, Annieck M. Neirinck, Jana Perez-Andres, Martín De Maertelaere, Emilie Berkowska, Magdalena A. Kerre, Tessa Hofmans, Mattias Orfao, Alberto van Dongen, Jacques J. M.
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Published in
Frontiers in Immunology
Background Multiparameter flow cytometry (FC) is essential in the diagnostic work-up and classification of primary immunodeficiency (PIDs). The EuroFlow PID Orientation tube (PIDOT) allows identification of all main lymphocyte subpopulations in blood. To standardize data analysis, tools for Automated Gating and Identification (AG&I) of the informat...
Kriegsmann, Katharina Hundemer, Michael Hofmeister-Mielke, Nicole Reichert, Philipp Manta, Calin-Petru Awwad, Mohamed H.S. Sauer, Sandra Bertsch, Uta Besemer, Britta Fenk, Roland
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Published in
Cancers
In order to meet the challenges in data evaluation and comparability between studies in multiple myeloma (MM) minimal residual disease (MRD) assessment, the goal of the current study was to provide a step-by-step evaluation of next-generation sequencing (NGS) and multicolor flow cytometry (MFC) data. Bone marrow (BM) sample pairs from 125 MM patien...
