Sondergaard, Claus Svane Witt, Russell Mathews, Grant Najibi, Skender Le, Lisa Clift, Tracy Si, Ming-Sing
Published in
Cell and Tissue Research
Establishing vascularization is a critical obstacle to the generation of engineered heart tissue (EHT) of substantial thickness. Addition of endothelial cells to the formative stages of EHT has been demonstrated to result in prevascularization, or the formation of capillary-like structures. The detailed study of the effects of prevascularization on...
Crocini, Claudia Arimura, Takuro Reischmann, Silke Eder, Alexandra Braren, Ingke Hansen, Arne Eschenhagen, Thomas Kimura, Akinori Carrier, Lucie
Published in
Basic Research in Cardiology
Hypertrophic cardiomyopathy (HCM) is a myocardial disease associated with mutations in sarcomeric genes. Three mutations were found in ANKRD1, encoding ankyrin repeat domain 1 (ANKRD1), a transcriptional co-factor located in the sarcomere. In the present study, we investigated whether expression of HCM-associated ANKRD1 mutations affects contractio...
Friedrich, Felix W. Reischmann, Silke Schwalm, Aileen Unger, Andreas Ramanujam, Deepak Münch, Julia Müller, Oliver J. Hengstenberg, Christian Galve, Enrique Charron, Philippe
...
Published in
Basic Research in Cardiology
Based on evidence that FHL2 (four and a half LIM domains protein 2) negatively regulates cardiac hypertrophy we tested whether FHL2 altered expression or variants could be associated with hypertrophic cardiomyopathy (HCM). HCM is a myocardial disease characterized by left ventricular hypertrophy, diastolic dysfunction and increased interstitial fib...
Eder, Alexandra Hansen, Arne Uebeler, June Schulze, Thomas Neuber, Christiane Schaaf, Sebastian Yuan, Lei Christ, Torsten Vos, Marc A. Eschenhagen, Thomas
...
Published in
Basic Research in Cardiology
The assessment of proarrhythmic risks of drugs remains challenging. To evaluate the suitability of rat engineered heart tissue (EHT) for detecting proarrhythmic effects. We monitored drug effects on spontaneous contractile activity and, in selected cases, on action potentials (sharp microelectrode) and Ca2+ transients (Fura-2) and contraction under...
Eschenhagen, Thomas Reichenspurner, Herrmann Zimmermann, Wolfram Hubertus
Published in
Principles of Tissue Engineering
Tissue engineering of heart valves, small-diameter blood vessels, and myocardial patches is an exciting field with great clinical potential. The concerted research activity of several disciplines in the biomedical and engineering fields has yielded significant progress. It is still a major challenge to reproduce the exquisite natural design of any ...
Vollert, Ingra Eder, Alexandra Hansen, Arne Eschenhagen, Thomas
Published in
Current Stem Cell Reports
The human heart has very limited regenerative capacity, making the loss of heart muscle tissue during myocardial infarction essentially irreversible. Regenerative medicine aims at promoting the formation of new functional heart muscle in an injured heart, either by stimulating myocyte proliferation, formation of myocytes from preexisting stem cells...
Conradi, Lenard Schmidt, Stephanie Neofytou, Evgenios Deuse, Tobias Peters, Laura Eder, Alexandra Hua, Xiaoqin Hansen, Arne Robbins, Robert C Beygui, Ramin E
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Published in
Stem cells translational medicine
An initial insight into immunological consequences after transplantation of engineered heart tissue was gained through this study. Most important, this study was able to demonstrate cell survival despite rejection of matrix components. Generation of syngeneic human engineered heart tissue, possibly using human induced pluripotent stem cell technolo...
Eder, Alexandra Vollert, Ingra Hansen, Arne Eschenhagen, Thomas
Published in
Advanced drug delivery reviews
Drug development is time- and cost-intensive and, despite extensive efforts, still hampered by the limited value of current preclinical test systems to predict side effects, including proarrhythmic and cardiotoxic effects in clinical practice. Part of the problem may be related to species-dependent differences in cardiomyocyte biology. Therefore, t...
Denning, Chris Borgdorff, Viola Crutchley, James Firth, Karl S A George, Vinoj Kalra, Spandan Kondrashov, Alexander Hoang, Minh Duc Mosqueira, Diogo Patel, Asha
...
Published in
Biochimica et biophysica acta
Cardiomyocytes from human pluripotent stem cells (hPSCs-CMs) could revolutionise biomedicine. Global burden of heart failure will soon reach USD $90bn, while unexpected cardiotoxicity underlies 28% of drug withdrawals. Advances in hPSC isolation, Cas9/CRISPR genome engineering and hPSC-CM differentiation have improved patient care, progressed drugs...
Stillitano, Francesca Turnbull, Irene C. Karakikes, Ioannis Nonnenmacher, Mathieu Backeris, Peter Hulot, Jean-Sébastien Kranias, Evangelia G. Hajjar, Roger J. Costa, Kevin D.
Published in
European Heart Journal
In this study, we used three-dimensional human engineered cardiac tissue technology to directly show that phospholamban (PLN) R14del mutation impairs cardiac contractility and to demonstrate restoration of contractile properties with targeted genetic correction of this inheritable form of dilated cardiomyopathy.
