Published in Zeitschrift für Naturforschung C
Chemical analysis of the culture filtrates of Fusarium culmorum CMI 14764 has demonstrated the presence of seven trichothecene mycotoxins. Major metabolites are 3-acetyldeoxynivalenol and 7α,8α-dihydroxycalonectrin. with 3,15-diacetyldeoxynivalenol, deoxynivalenol. calonectrin, isotrichodermin and 12,13-epoxytrichothec-9-ene (EPT) as minor products...
Published in Mycotoxin Research
The influence of inoculum size on aflatoxin B1 (AFB1), zearalenone (ZEN) and deoxynivalenol (DON) production was examined on irradiated corn kernels. Spore concentrations were determined in serial dilutions and adjusted to 10,102,103,105 and 106 spores/ml. Aflatoxin B1 production was dependent on the inoculum size. The high levels of aflatoxin B1 p...
Published in Mycopathologia
Thin layer chromatography (TLC) methods for identifying and quantifying deoxynivalenol (DON), fumonisin B1 (FB1) and zearalenone in grain samples were compared to immunoassay (ELISA) and high performance liquid chromatography (HPLC) methods to determine the reliability of the less expensive TLC. There was a very good agreement between levels of DON...
Published in Mycotoxin Research
The influence of water activity (aw) on deoxynivalenol accumulation in wheat at 25°C was studied. Gnotobiotic grains were conditioned at different aw levels, inoculated with a toxigenic Fusarium graminearum strain, and incubated for ten weeks. The highest accumulation of deoxynivalenol (1130 ug/kg) was detected at aw 0.980. At aw 0.945 and 0.925 th...
Published in Chromatographia
A gas chromatographic procedure for the quantitative estimation of T-2 toxin, which utilises a florisil SPE clean-up procedure, has been adapted for the quantitative analysis of both T-2 toxin and deoxynivalenol (DON), but using an high performance thin layer chromatographic quantification step. The method has been validated by spiking uncontaminat...
Published in Mycopathologia
Twenty samples of unpolished (rough) rice collected in Arkansas and Texas during the 1995 harvesting season from fields exhibiting Fusarium sheath rot disease or panicle blight were previously shown to include 8 samples positive for fumonisin B1(FB1) in the range 2.2–5.2 ppm, and moniliformin (MON), but no beauvericin (BEA), deoxynivalenol, its der...
Published in Mycotoxin Research
Effects of plant cropping measures on toxin production were investigated. The use of the plough reduced the DON level by 71%. Additionally, the choice of less sensitive varieties, as well as an adequate nitrogen fertilization showed a significant reduction of the DON content in grain. In non-ploughed systems, an ear application with fungicides redu...
Published in Mycotoxin Research
In the course of a Fusarium Monitoring for winter wheat in Bavaria five deoxynivalenol risk factors have been identified. The major Fusarium species wasF. graminearum, which usually has one disease cycle; under specific weather conditions obviously a bicyclic spread of the pathogen occurs.
Published in Mycotoxin Research
A total of 27Fusarium culmorum isolates from Germany and 41F. graminearum isolates from Kenya were investigated for aggressiveness and mycotoxin production on wheat ears. In addition, ergosterol content of the kernels from ears inoculated withF. graminearum was determined and theF. culmorum isolates were tested for mycotoxin productionin vitro. For...
Published in Mycotoxin Research
Development of deoxynivalenol (DON) in wheat with a low contamination withFusarium spp. was investigated under suboptimal storage conditions (17% and 20% grain moisture, 20°C). The influence of storage on the relative DNA content of potential DON producers was also determined. The DON contents were quantified using an ELISA. The Tox5 PCR was used f...
