Our initial objectives in this project were three-fold. I. Express functional antibodies in the chloroplast of Chlamydomonas reinhardtii II. Express functional antibodies in the nucleocytoplasmic compartment of C. reinhardtii and III. Define optimal parameters for the large scale culture of antibody producing strains of C. reinhardtii.
FR
Les a-glucanes phosphorylases sont des enzymes catalysant de façon réversible la dégradation des a-l,4 glucanes en glucose-l-phosphate en présence d'orthophosphate. On distingue chez les végétaux supérieurs 2 isoformes de phosphorylase: Pho1 à localisation plastidiale (possédant une insertion caractéristique dans sa séquence peptidique) et Pho2, cy...
Published in BBA - Bioenergetics
Chlamydomonas reinhardtii cultures, deprived of inorganic sulfur, undergo dramatic changes during adaptation to the nutrient stress [Biotechnol. Bioeng. 78 (2002) 731]. When the capacity for Photosystem II (PSII) O 2 evolution decreases below that of respiration, the culture becomes anaerobic [Plant Physiol. 122 (2000) 127]. We demonstrate that (a)...
Published in Archives of Biochemistry and Biophysics
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) catalyzes the rate-limiting step of CO 2 fixation in photosynthesis, but O 2 competes with CO 2 for substrate ribulose 1,5-bisphosphate, leading to the loss of fixed carbon. Interest in genetically engineering improvements in carboxylation catalytic efficiency and CO 2/O 2 specificity has fo...
Published in Russian Journal of Plant Physiology
A pCTVHyg plasmid was constructed in a unicellular green alga Chlamydomonas reinhardtii Dang. by using the hygromycin phosphotransferase gene (hpt) as a selectable marker and the Escherichia coli transposon Tn5 under the early SV40 viral gene promoter. CW-15 mutant cells devoid of cell walls were transformed by electroporation in an electric field ...
Published in Journal of Biological Physics
PELDOR (Pulsed Electron eLectron DOuble Resonance) was applied to determinethe distance of between YZand QA-inYD-less mutant of Chlamydomonas reinhardtiiin Tris-treatedand Zn-substituted preparation of photosystem II. The value of distance wasfound to be 34.5 ± 1 Â. A ‘2+1’ electron spin echo method has beenapplied to measure the orientation of the...
